ImageXpress Ultra Confocal High-Content Analysis System

See clearly now with true point-scanning confocal imaging for high-content screening

Unravel the complexities of biological systems with amazing confocal images. The ImageXpress® Ultra Confocal High Content Screening System is a true point-scanning confocal imaging system for live cell or fixed samples on slides or in multi-well plates. The system's integral MetaXpress Software puts you in charge of four solid-state lasers, five beamsplitters, three filter cube sets, X & Y resolution, four objectives, Z-focus, pinhole diameter, and more. When your brilliant science requires brilliant, true laser-scanning confocal imaging, our ImageXpress Ultra System offers the ultimate solution.

  • True laser-scanning confocal system ensures a diverse set of samples can be imaged with excellent signal-to-noise ratio.
  • Software configurable detection pinhole provides two orders of magnitude of Z-resolution flexibility (from <1 µm to > 100 µm) over fixed-pinhole confocal systems.
  • Simultaneous or sequential scanning with up to 4 solid state lasers facilitates simultaneous acquisition for optimal speed performance (up to 4 times faster than single laser). Unique sequential scanning is achieved in combination with high-resolution z-offset to correct for objective lens-induced chromatic aberrations.
  • Fully-automated 100 nm resolution voice coil-driven stages provide positioning accuracy to capture intricate sub-cellular features as well as monitor long structures, such as neurons, across high-resolution tiled or stitched images.
High-Content Screening Neurons
Images of neurons were acquired and tiled from 6 sites. Each site is a long, continuous scan
capturing a larger area with long neuronal features. Note that the neurites perfectly
align across mutiple sites for easy quatification with our software algorithms.
  • Software configurable field-of-view simplifies imaging of large objects like model organisms (C. elegans and zebrafish) and long structures similar to neuronal processes.
Long Continuous Scanning from High Content Confocal Microscopy System
Long scan of cells stained with a cytosolic marker (green) and nucle (red).  
With a 20X objective scan lengths of up to 25.6 mm are possible.
  • Industry-leading autofocus option provides fast, reliable focusing with a dedicated 690 nm focus laser and ultra-fast sensors that will not bleach your sample while decreasing read times up to 5-fold.
  • Custom design for screening enables capturing 25,000 wells per day in a 384-well microplate assay with a two color high quality assay (e.g., Transfluor® beta-arrestin translocation assay). Lower resolution assays can be performed in excess of 100,000 wells / day.
  • 4-position automated objective changer eliminates manual objective changes and allows automated switching of magnifications during a single experiment.
  • Universal plate holder facilitates image acquisition using any clear bottom plate from 6-well to 1536-well, in standard height, low profile, or low volume.
  • Walk-away, 24/7 automation is achieved with the Thermo CRS CataLyst Express robotic plate handling system, enabling users to schedule multiple runs of one to three batches of 15 plates.


  • Determine the mechanisms of action for the research of cancer, neurological disorders, viral infection, and other diseases with compound or RNAi screening 
  • Quantitate multiplexed cellular responses, such as apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, and cell signaling
  • Detect one to four fluorescent markers along with the ability to resolve their colocalization
Transfluor Assay Taken with Confocal High Content Screening System
Unstimulated Transfluor-expressing cells wtih
cytosolic distribution of beta arrestin GFP.
Transfluor-expressing Cells with Confocal High Content Screening System
Stimulated Transfluor-expressing cells with
beta-arrestin GFP that has redistributed to vesicles.

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for cell health, cell cycle, micronuclei, neurite outgrowth, angiogenesis, translocation, multi-wavelength cell scoring and granularity

Stem Cell Research

  • Gain insight into the complex molecular and genetic signaling events that underlie cell proliferation and differentiation
  • Accurately measure nuronal process across multiple fields of view using unique stitching algorithm with precise stage movements
  • Analyze 3D samples by capturing an image stack in the Z-plane within each field of view and collapse it into a best-focus image or view the sample in 3D

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth, translocation, cell scoring and multi wavelength cell scoring.

Thick samples

  • Image neurons or stem cells grown on 3D matrices that can make focusing on the cells of interest a challenge  
  • Capture difficult-to-image thick tissue sections (at least 20 µm thick) that produce high-background fluorescence when stained
  • Analyze 3D samples by collecting a stack of crisp images in the Z-plane in as small as 100 nm increments
Neuronal Imaging Assays Analyses - High Content Analysis
Neuronal processes can be measured and
quantified with a Neurite Outgrowth Application
Module in MetaXpress Software.
Laser Scanning Confocal System - Thick Tissue Sections
Mouse kidney section stained for
nuclei (blue), actin filaments (green) and
plasma membrane (red)

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth and angiogenesis application modules.

Click here to learn more about MetaXpress Application Modules that enable some common HCS assays.  

Molecular Devices brings the image acquisition and analysis for ImageXpress Systems under one common platform to streamline the HCS workflow, offering a truly end-to-end solution for high-content screening.

MetaXpress® High-Content Image Acquisition & Analysis Software

  • MetaXpress Software Application Modules: turnkey solutions for your 100 most common analysis routines
  • MetaXpress Software Custom Modules: flexible toolbox for advanced assay development
  • MetaXpress Software Journals: powerful macros to analyze unique applications

AcuityXpress High-Content Informatics Software further analyzes, visualizes, and mines data.

MetaXpress® PowerCore High-Content Distributed Image Analysis Software dramatically accelerates analysis speed 25 times faster than acquisition in a high-throughput environment.

MDCStore Data Management Solution provides a secure access, management, and depolyment of a high-content analysis environment.

For product configurations and pricing on ImageXpress Ultra System, please please request a quote or contact us