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High-content confocal imaging solution with water objective options

The ImageXpress® Micro Confocal system is a high-content solution that can switch between widefield and confocal imaging of fixed and live cells. It can capture high quality images of whole organisms, thick tissues, 2D and 3D models, and cellular or intracellular events. The spinning disc confocal and sCMOS camera enable imaging of fast and rare events like cardiac cell beating and stem cell differentiation. With MetaXpress software and flexible options like water immersion objectives to choose from, the system enables many confocal imaging applications from 3D assay development to screening.

  • Acquire higher quality images

    Acquire higher quality images

    Capture excellent contrast, high resolution images with our proprietary AgileOptix™ spinning disc confocal technology, wide field of view, and bright light source.

  • Customize image acquisition and analysis

    Customize image acquisition and analysis

    Take ultimate control over acquisition and analysis parameters, which enables many applications from 3D structure analysis to target imaging of specific objects within an organism or cell populations. Run more applications with standard options like water immersion objectives, environmental control, or any of our high-performance customizations.

  • Analyze more data in less time

    Analyze more data in less time

    MetaXpress® PowerCore™ software accelerates analysis speed in a high-throughput environment. The software distributes image processing jobs to a multi-CPU environment.

ImageXpress Micro Confocal Virtual Tour

ImageXpress Micro Confocal Virtual Tour

Features

  • Wide dynamic range

    Quantify low and high intensity signals in a single image with >3 log dynamic range intensity detection.

  • Exclusive AgileOptix spinning disc confocal

    The technology provides increased sensitivity with custom optics, high-powered, solid state light engines, and sCMOS technology. Swappable disc geometries provide flexibility between speed and resolution.

  • Wide field of view

    Wide field of view enables whole-well confocal imaging and eliminates missed targets.

  • Optional on-board robotic fluidics

    For assays that involve compound addition, well washing, and media exchange, optional on-board robotic fluidics are available.

  • Accurate 3D measurements

    MetaXpress 3D analysis module is optimized for confocal imaging, enabling 3D measurements of volume and distance.

  • Multiple imaging modes

    The system offers phase contrast and brightfield label-free imaging, fluorescence, widefield, colorimetric, and confocal imaging, as well as, water immersion imaging as a standard option.

*NEW* Optimized imaging capabilities using standard options and high-performance customizations

Get optimized imaging capabilities† including water immersion objectives, high intensity lasers, and deep tissue penetrating, confocal disk module for live cell imaging, hypoxia studies, thick cell samples, and multiplexed assays.

AWES Illustration
Learn more

 

 

 

 

Gain deeper insights into cellular 3D structures with water immersion objectives

This video presentation details how high-performance water immersion objectives on the ImageXpress Micro Confocal system enhance the resolution, sensitivity and throughput for a variety of complex 3D cell-based assays.

*As a supporter of, and participant in, ISSCR’s 2020 Virtual, we share with you our Innovation Showcase presentation. This presentation does not represent an endorsement from or support of the ISSCR.

Gain deeper insights

 

 

 

 

Cellular Image Gallery

Micro Confocal High-Content Imaging System
ImageXpress High-content imaging solution
ImageXpress Micro Confocal Imaging System
ImageXpress Micro Imaging System
Micro Confocal Imaging System
 
†Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

 

 

 

 

Introducing the MetaXpress 3D Analysis Toolkit

MetaXpress high-content image aquisition and analysis software

 

  • Meet high throughput requirements with a scalable, streamlined workflow
  • Adapt your analysis tools to tackle your toughest problems, including 3D analysis
  • Schedule automatic data transfer between third-party hardware sources and secure database
  • Set up hundreds of routinely used HCS assays using MetaXpress software modules
Learn More View Modules
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Applications of ImageXpress Micro Confocal High-Content Imaging System

  • 3D Cell Imaging and Analysis

    3D Cell Imaging and Analysis

    Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. Now you can generate more predictive data by incorporating technologies like the ImageXpress system with the integrated 3D Analysis Module in MetaXpress® software. This single interface will enable you to meet 3D acquisition and analysis challenges without compromise to throughput or data quality, giving you confidence in your discoveries.

    Learn More 

    Cancer Research

    Cancer Research

    Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.

    Learn More  

  • Cell Counting

    Cell Counting

    Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division rely on the assessment of the number or density of cells in a well.

    Learn More 

    Cell Migration Assays

    Cell migration assays

    The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell timelapse imaging.

    Learn More 

  • Cells in Extra-Cellular Matrices

    3d-hydrogels

    One common way of culturing cells in three dimensional space is through the use of extracellular matrix-based hydrogels, such as Matrigel. Cells are grown in an extracellular matrix (ECM) to mimic an in vivo environment. Differences between Matrigel and 2D cell cultures can be readily seen by their different cell morphologies, cell polarity, and/or gene expression. Hydrogels can also enable studies on cell migration and 3D structure formation, such as endothelial cell tube formation in angiogenesis studies.

    COVID-19 and Infectious Disease Research

    COVID-19 and Infectious Disease Research

    Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.

    Learn More 

  • Live Cell Imaging

    Live Cell Imaging

    Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.

    Learn More  

    Neurite Outgrowth / Neurite Tracing

    Neurite Outgrowth

    Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.

    Learn More  

  • Organ-on-a-Chip

    Organ on a Chip Assays

    Emulating organ physiology by co-culturing cells in a supportive 3D matrix and using microfluidic channels to perfuse nutrients or compounds over the resulting cellular structures, is rapidly gaining popularity as a biologically relevant screening model for new drugs or toxicity. The ImageXpress system provides a turnkey solution for both 2D or 3D applications and can accommodate many different types of slide and plate formats.

    Spheroids

    Spheroids

    Spheroids are multi-cellular 3D spheres which mimic in vivo cell responses and interactions. They have the ability to be highly reproducible and to be scaled for high-content screening. Compared with adherent cells grown in 2D monolayers, 3D growth conditions are believed to more closely reflect the natural environment of cancer cells. Acquiring measurements from these larger structures involve acquiring images from different depths (z-planes) within the body of the spheroid and analyzing them in 3D or collapsing the images into a single 2D stack before analysis.

  • Stem Cell Research

    Stem Cell Research

    Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress Nano system paired with CellReporterXpress software for analysis has utility in all parts of the stem cells researcher’s workflow from tracking differentiation to quality control to measuring functionality of specific cell types.

    Learn more 

    Toxicity Screening

    Toxicity Screening

    Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.

    Read Application Note 

Specifications & Options of ImageXpress Micro Confocal High-Content Imaging System

Resources of ImageXpress Micro Confocal High-Content Imaging System

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Presentations
Videos & Webinars
Water Immersion Objectives & High-Content Imaging

Gain deeper insights into cellular 3D structures with water immersion objectives

AI based approach to phenotypic characterization

An AI-based approach to high-content phenotypic characterization of human iPSC-derived neuronal cells

Implementing 3D Neural Spheroids

Implementing 3D Neural Spheroids in Drug Discovery

Accelerate your Screening with Automated Imaging

Accelerate your screening with high-content and automated imaging

Microplate based Detection

Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening

ImageXpress Micro Confocal Virtual Tour

ImageXpress Micro Confocal Virtual Tour

Magnetic 3D

Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow

Labtube Meets SLAS

LabTube Meets Molecular Devices & MIMETAS, Susan Murphy & Sebastiaan Trietsch

Plate annotation and curve fitting in MetaXpress

Plate annotation and curve fitting in MetaXpress

Plate acquisition on ImageXpress Micro Confocal using MetaXpress

Quickstart Guide: Plate acquisition on the ImageXpress Micro Confocal using MetaXpress

Review images on ImageXpress Micro Confocal using MetaXpress

Quickstart Guide: Review images on the ImageXpress Micro Confocal using MetaXpress

Image analysis on ImageXpress Micro Confocal using MetaXpress

Basic workflow from image acquisition to analysis on the ImageXpress Micro Confocal using MetaXpress

Developing High Throughput Organ on a Chip Tissue

Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging

iPSC Derived Cardiomyocytes and Neuronal Spheroids

Toxicity studies of iPSC-derived cardiomyocytes and neuronal spheroids

3D in Vitro Models

Optimizing high-content screening tools for physiologically-relevant 3D in vitro models

Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform

Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform

3D Imaging of Cancer Spheroids

3D Imaging of Cancer Spheroids

High-Content Screening for Identifying miRNAs

High-Content Screening for Identifying miRNAs

Identification of Selective Inhibitors of the STAT3 Signaling Pathway

Identification of Selective Inhibitors of the STAT3 Signaling Pathway

Oliver Kepp and Jayne Hesley - Detect Cell Death Signatures with High Content Imaging

Oliver Kepp and Jayne Hesley - Hallmarks of Cancer - Detect and Quantify Cell Death Signatures with High Content Imaging

Multidimensional ImageXpress Micro Confocal System

Multidimensional High-Throughput Imaging with the New ImageXpress Micro Confocal System

Pushing the Boundaries of High Content Screening

Pushing the Boundaries of High Content Screening

Preparing Assays for Genome-wide RNAi Screens

Preparing Assays for Genome-wide RNAi Screens Using High Content Microscopy

Multiplexed High Content Hepatoxicity Assays

Multiplexed High Content Hepatoxicity Assays Using iPSC-Derived Hepatocytes

High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing in Virto Tissue Models

High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 Tissue Models

Simple and Flexible High Content Imaging of Complex Biological Events

Simple and Flexible High Content Imaging Enabling Quantitation of Complex Biological Events

Contemporary Automation and High Content Imaging Tools

Contemporary Automation and High Content Imaging Tools for Screening Stem Cell-Derived Cardiomyocytes

3D Image Analysis from Subcellular Structures to Spheroids

Implement High-Throughput 3D Image Analysis for Samples from Subcellular Structures to Spheroids

Live Cell Imaging to Investigate Cell Division Timing

Live Cell Imaging to Investigate the Regulation of Cell Division Timing

High-throughput RNA Screening to Identify Host Factors

Using High-throughput RNA Screening to Identify Host Factors That Impact Viral Infection

Application of HCA Tools for Antibody Drug Discovery

Application of HCA Tools for Antibody Drug Discovery

3D Spheroid Assays Using High-Content Imaging

Setting up 3D Spheroid Assays Using High-Content Imaging

Physiologically-relevant tissue models using a high-throughput Organ-on-a-Chip platform

Physiologically-Relevant Tissue Models Using a High-Throughput Organ-on-a-Chip Platform

Pluripotent Stem Cell Applications in Drug Discovery

Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery

StemoniX microBrain 3D Assay

StemoniX microBrain 3D Assay Ready Plates for HTS

Related Products & Services of ImageXpress Micro Confocal High-Content Imaging System

Expand your research using flexible imaging solutions

Molecular Devices provides flexible options for the ImageXpress Micro Confocal High-Content Imaging System to meet your research needs and to easily capture images from different sample formats, including hanging drops and in round or flat bottom plates, for monitoring cell health kinetics under environmental control, and more. With over 30+ years of imaging expertise, we can help you select the right options to ensure the best images for your assay.

*NEW*
Water Immersion Objectives

WATER IMMERSION
OBJECTIVES

20X, 40X, and 60X water immersion objectives improve the geometric accuracy during acquisition and reduce light refraction for brighter intensity at lower exposure times.

urnkey, high-throughput long term kinetics

ENVIRONMENTAL CONTROL

Environmental control maintains temperature and humidity levels while minimizing evaporation for multi-day, live cell, time-lapse imaging.

Phrase Contrast

TRANSMITTED LIGHT TOWER

Our transmitted light tower enables acquisition of high contrast images for unstained cells which can be easily viewed or separated from background.

 
Robotic Automation

ON-BOARD ROBOTIC
FLUIDICS

Integrated fluidics automates assay workflows which require compound addition, well washing, and media exchange.

 
*NEW*
Water Immersion Objectives

Water Immersion Objectives

Water immersion objectives can offer signal increases of up to 4 times which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

Water immersion objective

Streamline set up to get started more quickly

Water immersion objectives are field upgradeable and can be implemented with minimal change to current workflows.

Z-Resolution and Spherical Aberrations

Gain up to 4 times more signal at depth

Improve z-resolution and decrease optical aberrations for more accurate reconstruction of 3D samples.

Gain sharper, crisper images

Gain sharper, crisper images

Better light collection results in images with brighter intensity at lower exposure times, contributing to better quality of data.

Run your Samples with Confidence

Run your samples with confidence

MetaXpress software includes integrated, user-intuitive sensors and alerts to indicate the system health of the water controller system to give you peace of mind when running your samples.

High-throughput 3D screening

Acquire 3D samples at high throughput

The ImageXpress Micro Confocal system with water immersion objectives automatically replenishes the water during acquisition, so the entire microplate sample can be acquired at high throughput.

Simplify viewing and quantification of 3D structures

Simplify viewing and quantification of 3D structures

Analyze spheroids, microtissues, cells in a 3D matrix, and small organisms that are acquired as a stack of z-planes. Utilize the MetaXpress 3D Analysis Module to evaluate volume, XYZ location, distance to neighboring objects, diameter, depth, various intensity measurements, texture, or number of objects.


Shorten image acquisition time

Shorten image acquisition time

QuickID targeted acquisition images at low magnification to find objects or interest or rare events, then, automatically acquires high-magnification images with the required wavelengths, z-stack, or time points. This analysis process shortens acquisition time and reduces imaging storage requirements by acquiring selected targets at high-magnification.

Gain more insights using
high-performance customizations

Download Flyer
Image Imaging Widefield Confocal Comparison

 

 

Implement a solution that works for you

Molecular Devices can successfully tailor the ImageXpress Micro Confocal High-Content Imaging System to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support.

Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms

*NEW*
High Intensity Lasers

HIGH-INTENSITY
LASERS

Expand experiment capabilities with 5- or 8-channel high-intensity lasers.

*NEW*
Confocal Disk Module

DEEP TISSUE PENETRATING,
CONFOCAL DISK MODULE

The deep tissue penetrating, confocal disk module reduces crosstalk to improve out-of-focus light suppression and penetrate deeper into tissue.

Robotic Automation

SCALE UP ROBOTIC
AUTOMATION

Increase throughput, eliminate human errors, maintain sterility, and achieve consistent sample handling. Modular automation design—components can be added in modules and are upgradeable.

Automatic Pipettor

REAL-TIME DOSE
RESPONSE

Automatic pipettor enables compound addition while simultaneously live streaming at >100 frames per second.

urnkey, high-throughput long term kinetics

TURNKEY, HIGH-THROUGHPUT
LONG TERM KINETICS

Schedule and image multiple plates over long periods of time while keeping consistent temperature, O2 (Hypoxia), CO2, and humidity conditions. Expand live cell walk-away capacity to 200+ plates.

** Add media exchange for a turnkey high throughput organoid screening solution.

*NEW*
High Intensity Lasers

High-Intensity Lasers

High output laser excitation can reduce exposure times by up to 75%.† The 5-channel laser light source has an output of 400—1,000mw/channel. The 8-channel laser light source (including nIR) with 1,000mw/channel is ideal for customers with increased multiplexing requirements.

  • Obtain sharper images with higher signal-to-noise
  • Generate up to a 2X† boost in scan speed attributed to significantly reduced exposure times
  • Run FRET experiments using lasers for CFP and YFP
Standard
Standard Intensity Image
Laser
High-Intensity Lasers Image

Images taken at the same exposure.

*NEW*
Confocal Disk Module

Deep tissue penetrating, confocal disk module

An upgrade to the deep tissue penetrating, confocal disk module, combined with a laser light source, can improve resolution when imaging thick tissue samples†.

  • Improve suppression of out-of-focus light
  • Reduce haze (pinhole crosstalk)
  • Penetrate deeper into thick tissue samples for sharper images
Standard spinning disk
Standard Spinning Disc
Deep tissue penetrating, confocal disk module
Deep Tissue Penetrating with Confocal Disk

Images taken at the same exposure.

†Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

Featured Applications

3D Cell Imaging and Analysis

3D Cell Imaging and Analysis

Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue…

Read More
Cancer Research

Cancer Research

Cancer researchers need tools that enable them to more easily study the complex and often poorly…

Read More
Cell Counting

Cell Counting

Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug…

Read More
Cell Migration Assays

Cell Migration Assays

The movement or migration of cells is often measured in vitro to elucidate the mechanisms of…

Read More
Cells in Extra-Cellular Matrices

Cells in Extra-Cellular Matrices

One common way of culturing cells in three dimensional space is through the use of extracellular…

Read More
COVID-19 and Infectious Disease Research

COVID-19 and Infectious Disease Research

Here we've addressed common applications in infectious disease research including cell line…

Read More
Live Cell Imaging

Live Cell Imaging

Live cell imaging is the study of cellular structure and function in living cells via microscopy.…

Read More
Neurite Outgrowth / Neurite Tracing

Neurite Outgrowth / Neurite Tracing

Neurons create connections via extensions of their cellular body called processes. This biological…

Read More
Organ-on-a-Chip

Organ-on-a-Chip

Emulating organ physiology by co-culturing cells in a supportive 3D matrix and using microfluidic…

Read More
Spheroids

Spheroids

Spheroids are multi-cellular 3D spheres which mimic in vivo cell responses and interactions. They…

Read More
Stem Cell Research

Stem Cell Research

Pluripotent stem cells can be used for studies in developmental biology or differentiated as a…

Read More
Toxicity Screening

Toxicity Screening

Screening for off-target or toxic effects is very important during the development of new drugs and…

Read More

Customer Breakthrough

ImageXpress Micro Confocal 3D Disease Models
ImageXpress Micro Confocal 3D Disease Models

SUCCESS STORY

Bioneer use the ImageXpress Micro Confocal for high-throughput imaging of 3D disease models

Continue Reading

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Request Quote

Maximize flexibility in application workflow and experimental design

Contact our specialist today to discuss how to implement a solution that works for you

Request Quote

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Request Quote

Maximize flexibility in application workflow and experimental design

Contact our specialist today to discuss how to implement a solution that works for you

Request Quote
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