Stem Cell Research
Stem cells provide researchers with new opportunities to study targets and pathways that are more relevant to disease processes. They offer a more realistic model to identify and confirm new drug targets and generate pharmacology and toxicology data earlier, with stronger translation to the clinical setting. Additionally, the application of stem cells in drug development creates a new path to personalized medicine, while at the same time reducing, or even potentially replacing, animal testing.
Induced pluripotent stem cell-derived (iPSC-derived) cells enable researchers to study primary cells without the limitations traditionally encountered in obtaining such cells.
Benefits of stem cell research
The biopharma industry is continuing to adapt more cell-based assays for primary and secondary screening because of higher biological relevance and increased value of information. As part of this evolution, there is a desire to move from immortalized, stable cell lines to primary cells and stem cells. Stem cells offer the advantages of providing better clinical relevance of information compared to cell lines, being available in larger quantities, and having higher assay reproducibility than primary cells. Therefore, there is a great interest in automated stem cell assays to use as screening tools in early drug development, and to evaluate potential toxic effects of new compounds.
The workflows for assays involving stem cells are similar to other cell-based assays, but there are critical differences. Differentiation is often involved in stem cell assays because of the need to start with undifferentiated cells, or because the assay is studying the differentiation process. Monitoring the type and number of cells is often important when more than one lineage is present, or when both mature and undifferentiated cells are present. Accordingly, the data analysis and visualization requirements become more complex as multiple readouts from multiple cell types are required.
Stem cell imaging – High-content screening techniques and tools
Complex assays and 3D iPSC-derived cell models better represent tissue biology and cell interactions, making them more relevant for many toxicity and drug screening assays. As a result, the development of higher throughput quantitative assays using 3D cultures is an active area of investigation. Complicated 3D image acquisition and analysis workflows make it difficult to scale these assays for screening.
The ImageXpress® Micro High-Content Imaging Systems and MetaXpress® 3D Analysis Module allow us to implement a simpler workflow to quantify and visualize 3D structures.
Hepatotoxicity assay using 3D spheroid liver micro tissues derived from iCell Hepatocytes.
(A) iCell Hepatocytes were grown in 2D format for seven days prior to using them to prepare 3D cultures. Following spheroid formation, the liver micro tissues were treated with compounds for 72 hours, then stained for two hours and imaged. Z-planes were acquired using the ImageXpress Micro Confocal system.
(B) The acquired z-plane images were used to generate sets of 2D and 3D image segmentation, which were analyzed to quantify key phenotypic features of the 3D cultures.
Stem cell science insights and breakthroughs presented at #ISSCR2021
This year's ISSCR Annual Meeting brought leaders together from across the globe to discuss new technologies, share research insights, and explore the newest breakthroughs in stem cell science and regenerative medicine. With its impressive turnout, attendees learned how to get significant data from complex biological systems to reduce lab costs and push the boundaries of their research.
If you didn't get a chance to attend our Innovation Showcase and poster presentations during the show—we've got you covered. We've gathered all of our abstracts and poster sessions in a handy, one-stop-shop just for you.
Stem cell applications and the tools to transform your data into unique biological insights
The stem cell workflow demands a variety of complex assay types that monitor all phases from initial growth and expansion to differentiation, to quality control, and finally predictive assays for therapeutic research and drug discovery. Our suite of hardware and software solutions enable automated analysis of stem cells for many of the steps in the workflow. We are continuing to develop new techniques to help further condense and automate the workflow for stem cell assays.
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3D Cell Models
3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics. Utilization of 3D cellular assays adds value to research and screening campaigns, spanning the translational gap between 2D cell cultures and whole-animal models. By reproducing important parameters of the in vivo environment, 3D models can provide unique insight into the behavior of stem cells and developing tissues in vitro.
3D Cell Models
3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics. Utilization of 3D cellular assays adds value to research and screening campaigns, spanning the translational gap between 2D cell cultures and whole-animal models. By reproducing important parameters of the in vivo environment, 3D models can provide unique insight into the behavior of stem cells and developing tissues in vitro.
Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.
- Poster: Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
- Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
- Assaying cardiotoxicity with ImageXpress high-content screening systems
- High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
- Multiplexed automated imaging assays for compound testing using iPSC-derived cell models
- Paper: In vitro cardiotoxicity assessment of environmental chemicals using an organotypic human iPSC-derived model
Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.
- Poster: Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
- Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
- Assaying cardiotoxicity with ImageXpress high-content screening systems
- High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
- Multiplexed automated imaging assays for compound testing using iPSC-derived cell models
- Paper: In vitro cardiotoxicity assessment of environmental chemicals using an organotypic human iPSC-derived model
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Hematopoietic
Stem cells differentiated into hematopoietic progenitors (blood-forming stem cells) are important for both cell therapy and drug development. They can be used to study various blood disorders, such as leukemia or immune system disorders.
During the development of lymphoid and myeloid lineages, hematopoietic stem cells (HSCs) differentiate into committed hematopoietic progenitors. Monitoring the expansion and differentiation of HSCs into lineage-committed hematopoietic progenitors is important for research of hematopoiesis and developing therapeutic processes with HSCs.
Hematopoietic
Stem cells differentiated into hematopoietic progenitors (blood-forming stem cells) are important for both cell therapy and drug development. They can be used to study various blood disorders, such as leukemia or immune system disorders.
During the development of lymphoid and myeloid lineages, hematopoietic stem cells (HSCs) differentiate into committed hematopoietic progenitors. Monitoring the expansion and differentiation of HSCs into lineage-committed hematopoietic progenitors is important for research of hematopoiesis and developing therapeutic processes with HSCs.
Hepatocytes
Stem cells differentiated into hepatocytes, or liver cells, can be used to predict liver toxicity by drug candidates. Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus, highly predictive assays for safety and efficacy testing are crucial for improving drug development. Here, we demonstrate the development of multi-parametric hepatotoxicity assays utilizing the ImageXpress Micro system. Each well or cell yields multiple compound-induced cellular responses, which are then analyzed using custom modules from MetaXpress® High-Content Image Acquisition & Analysis Software.
Hepatocytes
Stem cells differentiated into hepatocytes, or liver cells, can be used to predict liver toxicity by drug candidates. Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus, highly predictive assays for safety and efficacy testing are crucial for improving drug development. Here, we demonstrate the development of multi-parametric hepatotoxicity assays utilizing the ImageXpress Micro system. Each well or cell yields multiple compound-induced cellular responses, which are then analyzed using custom modules from MetaXpress® High-Content Image Acquisition & Analysis Software.
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Human iPSC-derived 3D neural spheroids (Stemonix microBrain 3D)
To speed up the development of more effective and safer drugs, there is an increasing need for more complex, biologically relevant, and predictive cell-based assays for drug discovery and toxicology screening. Human iPSC-derived neural 3D co-cultures (StemoniX® microBrain® 3D platform) have been developed as a high-throughput screening platform that more closely resembles the constitution of native human cortical brain tissue. 3D neural spheroids contain a neural network enriched in synapses, creating a highly functional neuronal circuitry that displays spontaneous, synchronized, readily detectable calcium oscillations.
- Phenotypic characterization of neuroactive compound effects
- Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
- Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
- Webinar: Implementing 3D neural spheroids in drug discovery
- Poster: Multiplexed automated assays for neurotoxicity evaluation using iPSC cell-derived neural 3D cell models
Human iPSC-derived 3D neural spheroids (Stemonix microBrain 3D)
To speed up the development of more effective and safer drugs, there is an increasing need for more complex, biologically relevant, and predictive cell-based assays for drug discovery and toxicology screening. Human iPSC-derived neural 3D co-cultures (StemoniX® microBrain® 3D platform) have been developed as a high-throughput screening platform that more closely resembles the constitution of native human cortical brain tissue. 3D neural spheroids contain a neural network enriched in synapses, creating a highly functional neuronal circuitry that displays spontaneous, synchronized, readily detectable calcium oscillations.
- Phenotypic characterization of neuroactive compound effects
- Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
- Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
- Webinar: Implementing 3D neural spheroids in drug discovery
- Poster: Multiplexed automated assays for neurotoxicity evaluation using iPSC cell-derived neural 3D cell models
Imaging cytometry enables complex cell-based assays
The ability to combine a general cell health assay with a measurement of a specific effect increases confidence in the quality of toxicity results. The SpectraMax® MiniMax™ 300 Imaging Cytometer is an intuitive platform that supplements microplate reader assays with imaging cytometry to provide more biologically relevant data to non-imaging specialists. Here, we present results of viability and toxicity assays using iPSC-derived cells performed on the imaging cytometer.
Imaging cytometry enables complex cell-based assays
The ability to combine a general cell health assay with a measurement of a specific effect increases confidence in the quality of toxicity results. The SpectraMax® MiniMax™ 300 Imaging Cytometer is an intuitive platform that supplements microplate reader assays with imaging cytometry to provide more biologically relevant data to non-imaging specialists. Here, we present results of viability and toxicity assays using iPSC-derived cells performed on the imaging cytometer.
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Live cell time-lapse imaging
Live cell time-lapse imaging provides the ability to monitor responses in living cells over a specific period of time and offers researchers key advantages for assay development. For routine cell-based screening, time-course results can determine the correct time to read endpoint assays. Here, we demonstrate how high-content time-lapse imaging can be used to characterize cell health kinetics and to monitor cell proliferation or death of iPSC-derived neurons.
Live cell time-lapse imaging
Live cell time-lapse imaging provides the ability to monitor responses in living cells over a specific period of time and offers researchers key advantages for assay development. For routine cell-based screening, time-course results can determine the correct time to read endpoint assays. Here, we demonstrate how high-content time-lapse imaging can be used to characterize cell health kinetics and to monitor cell proliferation or death of iPSC-derived neurons.
Neuronal stem cell expansion and differentiation
There is great interest in using neuronal cells as screening tools in early drug development. However, assays that monitor the expansion and differentiation of neuronal stem cells are complex, and manual analysis can be laborious and inaccurate. Here, we demonstrate a streamlined high-content imaging and analysis workflow to automatically evaluate stem cell expansion and quantitate the extent of differentiation.
- Monitoring neuronal stem cell expansion and differentiation using high-content imaging solutions
- Poster: Novel high content stem cell-based assays for screening
- Poster: High content analysis of neural stem cell expansion and differentiation
Neuronal stem cell expansion and differentiation
There is great interest in using neuronal cells as screening tools in early drug development. However, assays that monitor the expansion and differentiation of neuronal stem cells are complex, and manual analysis can be laborious and inaccurate. Here, we demonstrate a streamlined high-content imaging and analysis workflow to automatically evaluate stem cell expansion and quantitate the extent of differentiation.
- Monitoring neuronal stem cell expansion and differentiation using high-content imaging solutions
- Poster: Novel high content stem cell-based assays for screening
- Poster: High content analysis of neural stem cell expansion and differentiation
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Neurons, neurite outgrowth
Stem cells differentiated into neurons are often used in drug discovery to study central nervous system (CNS) diseases such as Alzheimer's, schizophrenia, and anxiety. These neurons can also be used in toxicological assays to help predict drug safety and efficacy.
- Identify compound-specific effects on neurite outgrowth using ImageXpress Pico system
- Assessment of neurotoxicity and neuronal development using iPSC-based neurite outgrowth assay
- High-content assay for morphological characterization of neuronal development in 3d matrix using human ipsc-derived neuronal cultures
Neurons, neurite outgrowth
Stem cells differentiated into neurons are often used in drug discovery to study central nervous system (CNS) diseases such as Alzheimer's, schizophrenia, and anxiety. These neurons can also be used in toxicological assays to help predict drug safety and efficacy.
- Identify compound-specific effects on neurite outgrowth using ImageXpress Pico system
- Assessment of neurotoxicity and neuronal development using iPSC-based neurite outgrowth assay
- High-content assay for morphological characterization of neuronal development in 3d matrix using human ipsc-derived neuronal cultures
Neurotoxicity, neuronal toxicity
High-content imaging using iPSCs of human origin can be applied to examine neurotrophic, neuroprotective, or neurotoxic effects of pharmaceutical drug candidates or environmental contaminants. This note demonstrates neuronal toxicity screening via automated endpoint and live-cell assays using iPSCs and Molecular Devices instruments and software.
- High-content screening of neuronal toxicity using iPSC-derived human neurons
- Poster – Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
- Webinar: Human iPS cell-derived neurons: a new physiologically relevant model for drug discovery applications for high-content screening of neurotoxicity
- Webinar: An AI-based approach to high-content phenotypic characterization of human iPSC-derived neuronal cells
Neurotoxicity, neuronal toxicity
High-content imaging using iPSCs of human origin can be applied to examine neurotrophic, neuroprotective, or neurotoxic effects of pharmaceutical drug candidates or environmental contaminants. This note demonstrates neuronal toxicity screening via automated endpoint and live-cell assays using iPSCs and Molecular Devices instruments and software.
- High-content screening of neuronal toxicity using iPSC-derived human neurons
- Poster – Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
- Webinar: Human iPS cell-derived neurons: a new physiologically relevant model for drug discovery applications for high-content screening of neurotoxicity
- Webinar: An AI-based approach to high-content phenotypic characterization of human iPSC-derived neuronal cells
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Organoids
Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures.
Organoids
Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures.
Phenotypic screening
eBook: Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Learn how to use both imaging and calcium oscillation analysis to develop profiles of compounds in iPSC-derived cardiomyocytes such as hERG blockers, ß-adrenergic agonists, and environmental toxins. iPSC-derived neuronal cultures were evaluated with neuromodulators as well as environmental toxins.
Phenotypic screening
eBook: Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Learn how to use both imaging and calcium oscillation analysis to develop profiles of compounds in iPSC-derived cardiomyocytes such as hERG blockers, ß-adrenergic agonists, and environmental toxins. iPSC-derived neuronal cultures were evaluated with neuromodulators as well as environmental toxins.
Resources of Stem Cell Research
Blog
Stem Cell Science and Regenerative Medicine – Technology and Methods Presented at ISSCR 2022
Stem Cell Science and Regenerative Medicine – Technology and Methods Presented at ISSCR 2022
It was another great year at ISSCR 2022! Leaders from across the globe came together to discuss new technologies, share insights, and explore the newest breakthroughs in stem cell…
Application Note
Neurite outgrowth analysis in 3D reconstructed human brain micro-tissues
Neurite outgrowth analysis in 3D reconstructed human brain micro-tissues
Organotypic 3D cultures resemble native 3D tissue architecture and are believed more representative of real tissues than their 2D (monolayer) counterparts, thus providing higher in vivo…
Publications
Benefits of Real-Time Live-Cell Imaging
Benefits of Real-Time Live-Cell Imaging
Three-dimensional (3D) cell-based assays replace a host of tissue, cell, and animal test models but have a reputation for lacking time resolution. For example, traditional, endpoint cell-bas…
Scientific Poster
Intestinal organoids for automated screening assays. High content imaging and analysis of organoid morphology
Intestinal organoids for automated screening assays. High content imaging and analysis of organoid morphology
Here we describe a workflow for automation of organoid culture. The automated method utilizes an integrated work-cell, consisting of several instruments providing automated cell culture, mon…
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Application Note
Label-free cell segmentation with IN Carta SINAP application module
Label-free cell segmentation with IN Carta SINAP application module
Label-free cell analysis provides a better alternative to using fluorescent dyes as it enables scientists to image live cells under near-native conditions – examining biological processes…
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Blog
Engineering Next-gen Organoids with Automated Lab Workflows at #SLAS2022
Engineering Next-gen Organoids with Automated Lab Workflows at #SLAS2022
SLAS2022, the Society for Lab Automation and Screening conference offered another exciting year for learning about innovative laboratory technologies. Whether you attended in-person…
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Application Note
Improving the robustness of Cell Painting with a near-infrared label and advanced image and data analytics
Improving the robustness of Cell Painting with a near-infrared label and advanced image and data analytics
Image-based phenotypic profiling approaches such as the widely used Cell Painting assay use high-content imaging along with multiparametric readouts to study biological, genetic, and…
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Blog
Stem cell science insights and breakthroughs presented at #ISSCR2021
Stem cell science insights and breakthroughs presented at #ISSCR2021
If you didn't get a chance to visit us at our poster sessions during ISSCR 2021, don’t fret. We've gathered all our sessions right here for you. The ISSCR Annual Meeting brought…
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Scientific Poster
Organoids for disease modeling and in vitro drug screening
Organoids for disease modeling and in vitro drug screening
3D cell models representing various tissues were successfully used for studying complex biological effects, tissue architecture, and functionality. However, the complexity of 3D models remai…
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Scientific Poster
Monitoring organoid development and characterization of calcium oscillation activities in iPSC-derived 3D cerebral organoids
Monitoring organoid development and characterization of calcium oscillation activities in iPSC-derived 3D cerebral organoids
Cerebral organoids are a rapidly developing technology that has great potential for understanding brain development and neuronal diseases. They can also be used for testing effects of compou…
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Scientific Poster
High-throughput assessment of compound-induced pro-arrhythmic effects in human IPSC-derived cardiomyocytes
High-throughput assessment of compound-induced pro-arrhythmic effects in human IPSC-derived cardiomyocytes
Development of biologically relevant and predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery. The focus of this study was to es…
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Scientific Poster
Deep learning-based image analysis for label-free live monitoring of iPSC 3D organoid cultures
Deep learning-based image analysis for label-free live monitoring of iPSC 3D organoid cultures
Complex 3D biological models such as organoids and patient derived spheroids are gaining popularity in many biomedical research areas because they more closely recapitulate the in vivo tissu…
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Publications
Project Profile: Organoid Innovation Center
Project Profile: Organoid Innovation Center
Lab Manager speaks to Dan O’Connor, vice president, drug discovery, Molecular Devices, about the company’s Organoid Innovation Center in San Jose, CA. The center is 180 sq. ft., within a lar…
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Blog
Tips for running a successful live cell imaging experiment
Tips for running a successful live cell imaging experiment
There have been significant advancements in microscopy and camera technology, as well as advancements in technologies for labeling molecules of interest over the past decade. These…
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Publications
Shaping the Future of Organoid Research
Shaping the Future of Organoid Research
Molecular Devices, a provider of innovative life science technology, recently unveiled a brand new, first-of-its-kind Organoid Innovation Center. Situated at the company’s global headquarter…
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Publications
Disease Modeling with 3D Cell-Based Assays Using a Novel Flowchip System and High-Content Imaging
Disease Modeling with 3D Cell-Based Assays Using a Novel Flowchip System and High-Content Imaging
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translational research. We describe here a novel automat…
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Publications
How AI is advancing neurodegenerative disease research
How AI is advancing neurodegenerative disease research
In this guest editorial, explore how artificial intelligence is becoming a powerful tool for drug discovery As part of the SelectScience Advances in Drug Discovery Special Feature, guest edi…
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Blog
Overcome the challenges of high-content cell analysis through AI/machine learning
Overcome the challenges of high-content cell analysis through AI/machine learning
Artificial intelligence (AI) is finding its way into many aspects of modern life, from autonomous vehicles to voice-powered personal assistants, and even the creation of art. But it…
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Blog
How Cell Painting is making its mark on drug discovery
How Cell Painting is making its mark on drug discovery
Have you ever heard the old adage, “A picture is worth a thousand words?” When it comes to Cell Painting, this saying is especially true. Cell Painting is a high-content,…
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Publications
Advanced Cell Screening and Imaging Reveals How the Cardiac System Responds in Drug Development
Advanced Cell Screening and Imaging Reveals How the Cardiac System Responds in Drug Development
In drug discovery, time and monetary investments increase significantly the further candidates advance through the preclinical phase of testing, analysis and evaluation. By excluding non-via…
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Scientific Poster
AI-based analysis of complex biological phenotypes
AI-based analysis of complex biological phenotypes
Cell-based phenotypic assays have become an increasingly attractive alternative to traditional in vitro and in vivo testing in pharmaceutical drug development and toxicological safety assess…
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Publications
The workflow game-changer: combined technologies for efficiency
The workflow game-changer: combined technologies for efficiency
Microfluidic flowchip technology combined with three-dimensional (3D) imaging is a powerful duo poised to change the future of drug discovery and development. The integration of combined tec…
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Publications
Neuroscience: bridging the gap between cell-based and human research
Neuroscience: bridging the gap between cell-based and human research
Neurological disorders impact up to a billion people around the world and appear to be on the rise [1]. In fact, those affecting the central and peripheral nervous systems – including stroke…
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Application Note
Phenotypic characterization of neuroactive compound effects
Phenotypic characterization of neuroactive compound effects
The FLIPR Penta system is powered by a high-speed EMCCD camera and the new ScreenWorks Peak Pro 2 software. The system allows measurement and analysis of complex patterns of calcium…
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Scientific Poster
Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
Cell-based phenotypic assays have become an increasingly attractive alternative to traditional in vitro and in vivo testing in pharmaceutical drug development and toxicological safety assess…
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Scientific Poster
Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
To speed up the development of more effective and safer drugs, there is an increasing need for more complex, biologically relevant and predictive cell-based assays for drug discovery and tox…
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Publications
LabTube Meets Molecular Devices & MIMETAS
LabTube Meets Molecular Devices & MIMETAS
Molecular Devices partners with MIMETAS to scale up organoid screening—helping researchers go from 2D to 3D phenotypic relevant organoid screening. At SLAS 2020, they featured the ImageXpres…
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Publications
The importance of the cellular microenvironment in the development of more efficient drugs against complex diseases
The importance of the cellular microenvironment in the development of more efficient drugs against complex diseases
The biotechnological start-up is focused on in vitro preclinical research and development, with a specialization in automated cellular imaging: high-content analysis (HCA) and high-content s…
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Application Note
Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Development of biologically relevant, predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery.
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Publications
Microenvironment and 3D image analysis key in the development of more efficient drugs against complex diseases
Microenvironment and 3D image analysis key in the development of more efficient drugs against complex diseases
The biotechnological start-up is focused on in vitro preclinical research and development with a specialization in cellular imaging: high-content analysis (HCA) and high-content screening (H…
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Application Note
Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
There is increasing interest in using more complex, biologically-relevant, and predictive cell-based platforms for assay development and compound screening.
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Publications
High-content screening of complex physiologically-relevant cell models
High-content screening of complex physiologically-relevant cell models
Recent years have seen an increasing demand for drug discovery and development processes to use more predictive, higher complexity, physiologically-relevant three-dimensional (3D) cell model…
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Application Note
Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
Develop functional and morphological readouts for testing effects of different compounds in multi-parametric assays using iPSC-derived cardiomyocytes
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Application Note
Identify compound-specific effects on neurite outgrowth using ImageXpress Pico system
Identify compound-specific effects on neurite outgrowth using ImageXpress Pico system
Evaluate compound-specific effects on neurite outgrowth using automated imaging
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Application Note
Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
Shorten growth timelines when screening compound effects on calcium transients in beating cardiomyocytes by using the iCell® Cardiomyocytes2 Kit (Cellular Dynamics International). iCell…
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Application Note
High-Content Assay for Morphological Characterization of Neuronal Development in a 3D Matrix using Human iPSC-Derived Neuronal Cultures
High-Content Assay for Morphological Characterization of Neuronal Development in a 3D Matrix using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cellbased assays for compound screening is a primary challenge in drug discovery. The development and integration of three…
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Application Note
Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
Growing concerns about the increased prevalence of untested chemicals in the environment has created a pressing need to develop reliable and efficient screening tools to identify chemicals…
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Scientific Poster
High Throughput Multiplexed Assay for Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity
High Throughput Multiplexed Assay for Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity
PDF reveals the ability of IsoCyte laser scanning system used with FCS Express software to qualify expression of lineage specific and stem cell markers of hematopoietic cells.
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Scientific Poster
Multiplexed Hepatotoxicity Assays using iCell® Hepatocytes
Multiplexed Hepatotoxicity Assays using iCell® Hepatocytes
Predictive in vitro assays suitable for safety testing are extremely important for reducing the incidence of late-stage drug attrition. We have paired human iPSCderived hepatocytes with the…
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Scientific Poster
Novel High Content Stem Cell-based Assays for Screening
Novel High Content Stem Cell-based Assays for Screening
The biopharma industry is continuing to adapt more cell-based assays for primary and secondary screening because of higher biological relevance and increased value of information.
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Scientific Poster
High Content Analysis of Neural Stem Cell Expansion and Differentiation
High Content Analysis of Neural Stem Cell Expansion and Differentiation
This application note describes the accurate image on the wells of this novel culture system with ImageXpress Micro High-Content Imaging Systems to visualize & quantify cytotoxic exposur…
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Scientific Poster
Novel Functional Phenotypes In Induced Pluripotent Stem Cell-derived Neurons From Patients With LRRK2 G2019S Mutation
Novel Functional Phenotypes In Induced Pluripotent Stem Cell-derived Neurons From Patients With LRRK2 G2019S Mutation
Virtually all experimental models aimed at mimicking Parkinson’s disease (PD) are limited in their ability to faithfully replicate disease phenotypes. A great hope relies on new patient-spec…
eBook
Stem Cell Imaging
Stem Cell Imaging
Scientists’ expanding knowledge and understanding of stem cells is transforming the way we research human biology and medicine. Stem cells’ ability to differentiate into a variety of human c…
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Application Note
Toxicity assays using induced pluripotent stem cell-derived cells
Toxicity assays using induced pluripotent stem cell-derived cells
Drug-induced organ toxicity is an important cause of pharmaceutical candidates failing to make it to market. Thus highly predictive assays for safety and efficacy testing are crucial for…
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Application Note
High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
Establishment of physiologically-relevant in vitro models is crucial to further understanding of the mechanisms of neurological diseases as well as targeted drug development. While iPSC-…
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Application Note
Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
Cardiotoxicity remains a primary cause for drug attrition during clinical trials and post-marketing. While it is believed that a significant percentage of cardiovascular diseases are due to…
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Application Note
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease,…
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Application Note
Selecting clones of mouse embryonic stem cells
Selecting clones of mouse embryonic stem cells
The targeted propagation of undifferentiated cells is of particular interest in mouse embryonic stem cell (mESC) culture and cell line development. The technology in ClonePix Systems lends…
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Application Note
High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
Eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process with the FLIPR Tetra System, together with ScreenWorks Peak Pro Software…
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Application Note
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
The assessment of cardiotoxicity is important in the early stages of drug discovery to enable elimination of potentially toxic compounds from further development. This is critical to reduce…
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Application Note
Assaying cardiotoxicity with ImageXpress high-content screening systems
Assaying cardiotoxicity with ImageXpress high-content screening systems
The discovery and development of novel drugs is a difficult and expensive process due to lack of tools and techniques to accurately reproduce the human physiological response in the…
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Application Note
High-content screening of neuronal toxicity using iPSC-derived human neurons
High-content screening of neuronal toxicity using iPSC-derived human neurons
The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. Neurotoxicity can cause temporary or…
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Application Note
Intelligent time-lapse imaging to enhance live cell assay development
Intelligent time-lapse imaging to enhance live cell assay development
The ability to monitor responses in living cells over a specific period of time offers cell biologists several key advantages for assay development. For routine cellbased screening, time-…
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Application Note
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus highly predictive assays for safety and efficacy testing are crucial for improving drug…
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Scientific Poster
In Vitro Cardiotoxicity and Neurotoxicity Assessment of Environmental Chemicals Using Organotypic Human Induced Pluripotent Stem Cell-Derived Models
In Vitro Cardiotoxicity and Neurotoxicity Assessment of Environmental Chemicals Using Organotypic Human Induced Pluripotent Stem Cell-Derived Models
An increased prevalence of neurological and cardiovascular disorders, possibly attributed to environmental toxicant exposures, has generated an interest in developing reliable and efficient…
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Scientific Poster
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is one of the main challenges in drug discovery. There is an increasing interest i…
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Scientific Poster
Complex Cell Based Assays with a Novel Imaging Cytometry System and Object Recognition Software
Complex Cell Based Assays with a Novel Imaging Cytometry System and Object Recognition Software
Learn how to run complex cell-based assays while maintaining high-quality multi-parametric data output using a novel imaging cytometry system and object recognition software.
Videos & Webinars
Enhance high-content 3D biology imaging with automated sample preparation
Enhancing 3D Disease Models: Automated, High-Throughput, Phenotypic Screening with Organ-on-a-Chip
Emerging Organoid Models: Translating Basic Research to Drug Development and Regenerative Medicine
ISSCR 2021 Innovation Showcase: Automated Culture and High-Content Imaging of 3D Lung and Cardiac
Organoid Innovation Center Walkthrough
Implementing 3D Neural Spheroids in Drug Discovery
Multi-parameter in vitro assessment of compound effects on cardiomyocyte physiology using induced pluripotent stem cells
Human iPS Cell-derived Neurons: A New Physiologically Relevant Model for Drug Discovery Applications for High-Content Screening of Neurotoxicity
Live Cell Imaging of Co-Cultured Stem Cells
Time-Lapse Cell Migration Assay of Stem Cells
Mouse Genetically-Modified Stem Cells via Transmitted Light and Fluorescence Imaging
High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
Live Cell Imaging to Investigate the Regulation of Cell Division Timing
Cell-Based Assays Using a Novel Plate Reader Platform with Imaging Cytometry
Related Products & Services of Stem Cell Research
Advanced Cloud-Based Analytics with StratoMineR
Increase efficiency and throughput with cloud-based advanced analytics
IN Carta Image Analysis Software
Provides robust, quantitative results from complex biological images and datasets
