Stem Cell Research
Stem cells models offer scientists the benefit of a more biologically relevant system. The FLIPR Tetra High-Throughput Cellular Screening System and imaging systems such as ImageXpress Micro Confocal and ImageXpress Micro 4 enable data acquisition and analysis in high-throughput and high-content environments.
Stem cells provide researchers with new opportunities to study targets and pathways that are more relevant to disease processes. They offer a more realistic model to identify and confirm new drug targets and generate pharmacology and toxicology data earlier, with stronger translation to the clinical setting. Additionally, the application of stem cells in drug development creates a new path to personalized medicine, while at the same time reducing, or even potentially replacing, animal testing. Induced pluripotent stem cell-derived (iPSC-derived) cells enable researchers to study primary cells without the limitations traditionally encountered in obtaining such cells.
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Application Note
Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
Shorten growth timelines when screening compound effects on calcium transients in beating cardiomyocytes by using the iCell® Cardiomyocytes2 Kit (Cellular Dynamics International).…
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Application Note
High-Content Assay for Morphological Characterization of Neuronal Development in a 3D Matrix using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cellbased assays for compound screening is a primary challenge in drug discovery. The development and integration of…
Tools used in Stem Cell Research
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Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.
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Hepatocytes
Stem cells differentiated into hepatocytes, or liver cells, can be used to predict liver toxicity by drug candidates.
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Hematopoietic
Stem cells differentiated into hematopoietic progenitors (blood-forming stem cells) are important for both cell therapy and drug development. They can be used to study various blood disorders, such as leukemia or immune system disorders.
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Neurons
Stem cells differentiated into neurons are often used in drug discovery to study central nervous system (CNS) diseases such as Alzheimer's, schizophrenia, and anxiety. These neurons can also be used in toxicological assays to help predict drug safety and efficacy.
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Embryonic Stem Cells
Embryonic stem cells (ES) are derived from embryos and are considered pluripotent, meaning able to differentiate into any cell type. There are currently two main cell types, mouse ES cells and human ES cells.
Resources of Stem Cell Research
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Application Note
Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
Shorten growth timelines when screening compound effects on calcium transients in beating cardiomyocytes by using the iCell® Cardiomyocytes2 Kit (Cellular Dynamics International). iCell…
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Application Note
High-Content Assay for Morphological Characterization of Neuronal Development in a 3D Matrix using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cellbased assays for compound screening is a primary challenge in drug discovery. The development and integration of three…
Scientific Poster
High Throughput Multiplexed Assay for Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity
PDF reveals the ability of IsoCyte laser scanning system used with FCS Express software to qualify expression of lineage specific and stem cell markers of hematopoietic cells.
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Scientific Poster
Novel Functional Phenotypes In Induced Pluripotent Stem Cell-derived Neurons From Patients With LRRK2 G2019S Mutation
Virtually all experimental models aimed at mimicking Parkinson’s disease (PD) are limited in their ability to faithfully replicate disease phenotypes. A great hope relies on new patient-spec…
eBook
Stem Cell Imaging
Scientists’ expanding knowledge and understanding of stem cells is transforming the way we research human biology and medicine. Stem cells’ ability to differentiate into a variety of human c…
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Application Note
Toxicity assays using induced pluripotent stem cell-derived cells
Drug-induced organ toxicity is an important cause of pharmaceutical candidates failing to make it to market. Thus highly predictive assays for safety and efficacy testing are crucial for…
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Application Note
High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
Establishment of physiologically-relevant in vitro models is crucial to further understanding of the mechanisms of neurological diseases as well as targeted drug development. While iPSC-…
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Application Note
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease,…
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Application Note
Selecting clones of mouse embryonic stem cells
The targeted propagation of undifferentiated cells is of particular interest in mouse embryonic stem cell (mESC) culture and cell line development. The technology in ClonePix Systems lends…
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Application Note
High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
Eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process with the FLIPR Tetra System, together with ScreenWorks Peak Pro Software…
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Application Note
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
The assessment of cardiotoxicity is important in the early stages of drug discovery to enable elimination of potentially toxic compounds from further development. This is critical to reduce…
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Application Note
High-content screening of neuronal toxicity using iPSC-derived human neurons
The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. Neurotoxicity can cause temporary or…
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Application Note
Multiplexed high content hepatotoxicity assays using iPSC-derived hepatocytes
Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus highly predictive assays for safety and efficacy testing are crucial for improving drug…
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Scientific Poster
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is one of the main challenges in drug discovery. There is an increasing interest i…
Videos and Demos
Multi-parameter in vitro assessment of compound effects on cardiomyocyte physiology using induced pluripotent stem cells
Human iPS Cell-derived Neurons: A New Physiologically Relevant Model for Drug Discovery Applications for High-Content Screening of Neurotoxicity
Live Cell Imaging of Co-Cultured Stem Cells
Time-Lapse Cell Migration Assay of Stem Cells
Mouse Genetically-Modified Stem Cells via Transmitted Light and Fluorescence Imaging
High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
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