Measure live cells migrating over time with cell migration assays using automated imaging
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Acquisition of discontinuous timelapse images
MetaXpress software allows the user to easily append acquisitions done at varying intervals into one timelapse experiment for ease of visualization and analysis.
Analyze cell area using transmitted light or fluorescence image
In this assay, cell proliferation, migration, and spreading all contributed to the cells’ ability to close the wound. Automated timelapse imaging using transmitted light or live cell-compatible fluorescence provided the means to measure the progress of the “healing”. The images from every time point may be objectively analyzed with high-content image analysis software so that the data can be plotted as a timecourse or evaluated individually at specific time points.
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Cell Migration Analysis with Oris Pro Assay
This assay uses a non-toxic biocompatible gel to form a cell-free zone in the center of the well. After cells are seeded, the biocompatible gel dissolves, allowing cells to migrate into the detection zone in the center of the well. For images acquired with the MiniMax cytometer, a region of interest corresponding to the detection zone is specified, and the level of cell migration (area covered by cells) is analyzed.
Evaluate Cell Migration with Fluoroblok
The SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. Image analysis provides accurate data compared to raw fluorescence, which is subject to cellular staining levels and background fluorescence. Using the image acquisition and analysis features of SoftMax Pro Software in combination with the 96-well FluoroBlok format, researchers can rapidly assess a wealth of cellular migration data to advance their studies in cancer and many other areas of biology.
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Make movies from timelapse images
With a few clicks in MetaXpress movies can be rendered from raw images, overlaid wavelengths, or analysis masks.
Measure Cell Migration
Cell migration, the relocation of cells, is relevant to wound healing, immunology, embryonic development, and irregular cellular events such as cancer metastasis. Cell migration assays are used to measure cell motility in a controlled environment, and both “scratch” assays and assays utilizing microplates with pre-formed “wounds” are frequently utilized for this purpose. By conducting the assay in a 96 or 384 well microplate, several compounds that may affect the cell’s ability to migrate, such as chemotherapeutic compounds or drugs to speed up wound healing, may be tested in one controlled experiment with adequate replicates.
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Objective Quantification of Cell Migration
Cell migration is a key process in normal development, immune function and wound healing. Abnormal cell migration is a feature of conditions such as cardiovascular disease and cancer. The CloneSelect Imager system reveals the in vitro effects of therapeutic candidates and cell culture matrices on cell migration as well as the study of cell-to-cell interactions. Migration application software in CloneSelect Imager enables measurement of cell migration based on objective cell detection.
Sort values to view your data of interest
The table analysis format can be used to view results for measurements of interest without having to look within individual wells. The table analysis format also allows users to sort values by columns.
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Visualize trends for measurements of interest
The scatter plot analysis format allows users to identify trends for measurements of interest between selected wells. In this example, we see that cells treated with Cytochalasin D did not migrate into the cell-free zone as completely as the untreated control.
Whole plate overview
A montage of thumbnails of low magnification images (4X) of an OrisPro Cell Migration Assay in 96 well format illustrates the benefit of a consistent cell-free zone for measuring cell migration.
Resources for Cell Migration Assays
Application Note
To achieve over 5 fold of human IgG protein detection signal enhancement with 1 round of CloneDetect K8495 assay optimization
To achieve over 5 fold of human IgG protein detection signal enhancement with 1 round of CloneDetect K8495 assay optimization
Protein G (Pro G) is a large cell surface binding protein that has a repeating 55-residue domain that binds with high affinity to the FC region of IgG. Together with Protein A (Pro A),…
Application Note
High-sensitive and affordable single-cell RNA sequencing
High-sensitive and affordable single-cell RNA sequencing
Single-cell RNA sequencing (scRNA-seq) is a state of the art technology for the unbiased characterization of gene expression in complex cell and tissue samples. Despite having…
Application Note
Detect endotoxin with the Kinetic-QCL Kinetic Chromogenic LAL Assay
Detect endotoxin with the Kinetic-QCL Kinetic Chromogenic LAL Assay
Monitoring for contaminants is a critical step during the production process in the pharmaceutical and medical device industries. A frequent contaminant, endotoxin, can cause fever,…
Application Note
3D Immune Cell Migration
3D Immune Cell Migration
Cell migration is an essential process for many biological phenomena such as early embryonic development and immune cell response. During inflammation, the recruitment of T cells through…
Publications
Project Profile: Organoid Innovation Center
Project Profile: Organoid Innovation Center
Lab Manager speaks to Dan O’Connor, vice president, drug discovery, Molecular Devices, about the company’s Organoid Innovation Center in San Jose, CA. The center is 180 sq. ft., within a lar…
Blog
Tips for running a successful live cell imaging experiment
Tips for running a successful live cell imaging experiment
There have been significant advancements in microscopy and camera technology, as well as advancements in technologies for labeling molecules of interest over the past decade. These…
Publications
The workflow game-changer: combined technologies for efficiency
The workflow game-changer: combined technologies for efficiency
Microfluidic flowchip technology combined with three-dimensional (3D) imaging is a powerful duo poised to change the future of drug discovery and development. The integration of combined tec…
Application Note
Measure cell migration using a simple scratch assay with timelapse live-cell imaging
Measure cell migration using a simple scratch assay with timelapse live-cell imaging
The movement or migration of cells has long been studied to elucidate the physiological mechanisms of angiogenesis, embryogenesis, cancer metastasis, immune responses, and wound healing.
Scientific Poster
Measure cell migration using automated cell imaging and analysis
Measure cell migration using automated cell imaging and analysis
Effectively image and analyze cell migration using a scratch assay or a dissolvable biocompatible gel
Application Note
Measure cell migration using discontinuous time-lapse imaging of live cells
Measure cell migration using discontinuous time-lapse imaging of live cells
Cell migration is an essential process required for many biological events including embryonic development, wound healing, cancer metastasis, and immunological responses.
Application Note
Evaluate cell migration with FluoroBlok inserts on the SpectraMax MiniMax cytometer
Evaluate cell migration with FluoroBlok inserts on the SpectraMax MiniMax cytometer
Cell migration, broadly defined as the movement of cells from one location to another, is important in diverse processes including embryonic development and wound healing. It is also a key…
Scientific Poster
High Content Analysis of an Automatable 3‐Dimensional Cell Invasion Assay
High Content Analysis of an Automatable 3‐Dimensional Cell Invasion Assay
Download this document for analysis of the Automatable 3 ‐Dimensional Cell Invasion Assay using ImageXpress Micro Widefield HCS System and MetaXpress and AcuityXpress Software.
Application Note
Objective quantification of cell migration on the CloneSelect Imager System
Objective quantification of cell migration on the CloneSelect Imager System
Cell migration is a key process in normal development, immune function and wound healing. Abnormal cell migration is a feature of conditions such as cardiovascular disease and cancer. The…
Application Note
Custom Module Editor For MetaXpress High Content Image Acquisition and Analysis Software
Custom Module Editor For MetaXpress High Content Image Acquisition and Analysis Software
The Custom Module Editor in version 5 of MetaXpress® Software is a flexible, interactive environment where users can create and test a template for image analysis. Galleries of processing…
Application Note
Cell migration analysis with Oris Pro assay on the SpectraMax MiniMax cytometer
Cell migration analysis with Oris Pro assay on the SpectraMax MiniMax cytometer
Cell migration, the movement of cells from one location to another, is a critical component of both normal and abnormal biological processes. The importance of cell migration in diverse…
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Make movies from timelapse images

Acquire a discontinuous time-lapse video on ImageXpress Micro