ImageXpress Pico Automated Cell Imaging System
Cellular imaging and analysis system that enables you to go from sample to results in minutes
Digital microscopy with automated brightfield, fluorescence, and Digital Confocal imaging
The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so you can start running experiments quickly. With features such as Digital Confocal* 2D on-the-fly deconvolution, Live Preview, and multi-wavelength cell scoring, the ImageXpress Pico offers you the ability to advance your discoveries in a small, affordable imager.
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Get started quickly
With the icon-driven, user-friendly CellReporterXpress® Image Acquisition and Analysis Software, your entire lab can streamline their digital microscopy. Start capturing and analyzing images with minimal training.
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Do more than cell counting
Expand your assays with over 25 preconfigured templates optimized for many cell-based experiments including apoptosis, mitochondrial evaluation, 3D cell models, live cell/timelapse, multiwavelength cell scoring, and neurite tracing.
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Automate imaging affordably
Alleviate the hassle of going to the core lab to run your samples. The system’s lab-friendly price allows researchers to afford the convenience of automated imaging and analysis on their lab bench. With options like Digital Confocal, environmental control and z-stack acquisition, the system can be ordered to fit your research.
ImageXpress Pico Automated Cell Imaging System – Feature Update
Features
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Multiple imaging modes
The ImageXpress Pico system offers objectives ranging from 4x to 63x and can operate in colorimetric, brightfield, fluorescence, or Digital Confocal 2D on-the-fly deconvolution imaging modes.
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Preconfigured analysis protocols
Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis. With features like the click-to-find tool, analysis parameters can be optimized by simply clicking on a few cells that match specific criteria.
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Plate-to-individual cell view
Data can be visualized at multiple levels from plate overview to individual cells. A wide variety of data visualization tools from the plate and cellular level empower users to learn as much as possible from their images and assays.
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Z-stack acquisition
Generate sharper images for more accurate segmentation using z-stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection.
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Quickly and easily identify regions of interest
Live Preview simplifies identification of regions of interest, letting users pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort.
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Environmental control
Multi-day, time lapse, and live cell assays can be run using the onboard environmental system with options for humidity, CO2, and O2 control. Optimized to prevent z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions.
Increase resolution with on-the-fly deconvolution
Significantly increase resolution and assay quality with Digital Confocal 2D on-the-fly deconvolution. The Digital Confocal Option restores light to its original point of origin, allowing you to decrease exposure time and improve statistical significance of your observations. Digital Confocal is seamlessly integrated into the ImageXpress Pico’s fluorescent image acquisition workflow allowing you to capture images with higher signal-to-noise data for more precise segmentation and analysis.
*ImageXpress Pico Digital Confocal uses AutoQuant 2D Real Time Deconvolution
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eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
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Brochure
ImageXpress Pico Automated Cell Imaging System
ImageXpress Pico Automated Cell Imaging System
The ImageXpress® Pico system does more than imaging—it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays.
Applications of ImageXpress Pico Automated Cell Imaging System
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Apoptosis Analysis
Apoptosis is a process of programmed cell death important to a number of biological processes including embryonic development and normal tissue maintenance. Disruption in the regulation of apoptosis has been implicated in various diseases including cancer. Biochemical events lead to characteristic changes in cell morphology such as cell shrinkage, nuclear fragmentation, chromatin condensation, and mRNA decay, and ultimately cell death. Apoptosis can be initiated via the intrinsic or extrinsic pathways, and both pathways induce cell death by activating caspase enzymes
Apoptosis Analysis
Apoptosis is a process of programmed cell death important to a number of biological processes including embryonic development and normal tissue maintenance. Disruption in the regulation of apoptosis has been implicated in various diseases including cancer. Biochemical events lead to characteristic changes in cell morphology such as cell shrinkage, nuclear fragmentation, chromatin condensation, and mRNA decay, and ultimately cell death. Apoptosis can be initiated via the intrinsic or extrinsic pathways, and both pathways induce cell death by activating caspase enzymes
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division have a need to assess the number or density of cells in a well. Automated imaging can greatly speed up the cell counting process while reducing manual labor and human errors. Cells can be counted using a variety of methods, such as label-free cell counting under transmitted light or detection of nuclear dye with fluorescent imaging.
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division have a need to assess the number or density of cells in a well. Automated imaging can greatly speed up the cell counting process while reducing manual labor and human errors. Cells can be counted using a variety of methods, such as label-free cell counting under transmitted light or detection of nuclear dye with fluorescent imaging.
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Digital Confocal
The ability of cells to adapt to their environment is mediated by highly controlled signaling pathways. In many cases, the initiation of these pathways by extracellular signals, such as hormones or cytokines, leads to the translocation of proteins from the cytoplasm to the nucleus. Once in the nucleus, these proteins can regulate the expression of target genes. Assays requiring precise identification of sub-cellular structures or molecules within different cellular compartments can benefit from the ability of image deconvolution to improve identification of the specific fluorescence signal against potentially high background caused by out-of-focus light. The ImageXpress Pico system with Digital Confocal 2D on-the-fly deconvolution is capable of measuring the translocation of proteins from the cytoplasm to the nucleus, like NF-κB translocation upon treatment with TNF-α. The enhancement in image resolution and maintenance of quantitative information from the image deconvolution leads to greater statistical significance in the analysis of nuclear translocation.
Digital Confocal
The ability of cells to adapt to their environment is mediated by highly controlled signaling pathways. In many cases, the initiation of these pathways by extracellular signals, such as hormones or cytokines, leads to the translocation of proteins from the cytoplasm to the nucleus. Once in the nucleus, these proteins can regulate the expression of target genes. Assays requiring precise identification of sub-cellular structures or molecules within different cellular compartments can benefit from the ability of image deconvolution to improve identification of the specific fluorescence signal against potentially high background caused by out-of-focus light. The ImageXpress Pico system with Digital Confocal 2D on-the-fly deconvolution is capable of measuring the translocation of proteins from the cytoplasm to the nucleus, like NF-κB translocation upon treatment with TNF-α. The enhancement in image resolution and maintenance of quantitative information from the image deconvolution leads to greater statistical significance in the analysis of nuclear translocation.
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
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Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
Resources of ImageXpress Pico Automated Cell Imaging System
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eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
-
Brochure
ImageXpress Pico Automated Cell Imaging System
ImageXpress Pico Automated Cell Imaging System
The ImageXpress® Pico system does more than imaging—it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays.
Scientific Poster
Cytotoxic analysis using iPSC-derived stem cell models
Cytotoxic analysis using iPSC-derived stem cell models
Automate complex cell-based assays with multi-parametric readouts while maintaining high data quality and precision.
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Scientific Poster
Phenotypic cell-based assays with a new automated imaging system and cell analysis software
Phenotypic cell-based assays with a new automated imaging system and cell analysis software
There is a great need to automate complex cell-based assays with multi-parametric read-outs while maintaining high data quality and precision. A compact, automated imaging system was us…
Application Note
Assess transduction efficiency of CFP-tagged nuclear protein with automated cellular imaging
Assess transduction efficiency of CFP-tagged nuclear protein with automated cellular imaging
Since the discovery of green fluorescent protein in the 1960s, a wide variety of fluorescent proteins (FP) and variants have been developed to span the entire visible light spectrum.…
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Application Note
Improve nuclear translocation assay results using image deconvolution
Improve nuclear translocation assay results using image deconvolution
Widefield microscopy is a powerful tool that has been utilized to investigate cellular and molecular processes involved in cell signaling.
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Application Note
Monitor multiple stages of apoptosis with live cell kinetic imaging
Monitor multiple stages of apoptosis with live cell kinetic imaging
The study of apoptosis is a critical aspect of drug discovery and development. Additionally, studying the relationship between apoptosis and other factors, such as oxidative stress, is…
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Application Note
Monitor cell proliferation and cell cycle in real time
Monitor cell proliferation and cell cycle in real time
There is an increased need to expand the variety and complexity of cell-based assays for biological research and drug discovery. Live-cell assays allow monitoring of cell responses in…
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Application Note
Evaluate anti-inflammatory compounds using a multiparametric THP-1 cell-based assay
Evaluate anti-inflammatory compounds using a multiparametric THP-1 cell-based assay
Macrophages originate from blood monocytes that leave the circulation and penetrate various tissues where they differentiate into macrophages.
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Application Note
Count cells with or without fluorescent labels using automated imaging
Count cells with or without fluorescent labels using automated imaging
The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation.
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Application Note
Measure cell migration using discontinuous time-lapse imaging of live cells
Measure cell migration using discontinuous time-lapse imaging of live cells
Cell migration is an essential process required for many biological events including embryonic development, wound healing, cancer metastasis, and immunological responses.
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Application Note
Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
Develop functional and morphological readouts for testing effects of different compounds in multi-parametric assays using iPSC-derived cardiomyocytes
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Application Note
Identify compound-specific effects on neurite outgrowth using ImageXpress Pico system
Identify compound-specific effects on neurite outgrowth using ImageXpress Pico system
Evaluate compound-specific effects on neurite outgrowth using automated imaging
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Application Note
Detection of autophagy using automated imaging
Detection of autophagy using automated imaging
Detect and quantify compound effects on the process of autophagy on the ImageXpress Pico system. The PC12 human neuroblastoma cell line was used as a model for assay development.
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Application Note
Multi-parametric assessment of cell phenotypes using ImageXpress Pico Automated Cell Imaging System
Multi-parametric assessment of cell phenotypes using ImageXpress Pico Automated Cell Imaging System
Automated cellular imaging is an efficient method for analyzing compound effects on cell phenotypes including morphology, viability, and marker expression.
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Application Note
Evaluation of mitochondrial integrity and mitochondria membrane potential using automated cell imaging and analysis
Evaluation of mitochondrial integrity and mitochondria membrane potential using automated cell imaging and analysis
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP)1,2.
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Application Note
Cytotoxicity assessment using automated cell imaging and live/dead assays
Cytotoxicity assessment using automated cell imaging and live/dead assays
Live/dead assays are utilized in a wide variety of research applications including investigation of cytotoxic effects of various compounds, treatments, or changes in gene expression.
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Application Note
Analysis of apoptosis using automated cell imaging
Analysis of apoptosis using automated cell imaging
Apoptosis is a process of programmed cell death that occurs in multicellular organisms.1,2 Biochemical events lead to characteristic changes in cell morphology and cell death.
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eBook
Cellular Imaging Insights
Cellular Imaging Insights
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
Flyer
Digital Confocal 2D Real-Time Deconvolution
Digital Confocal 2D Real-Time Deconvolution
Designed for the ImageXpress® Pico Automated Cell Imaging System, and utilizing CellReporterXpress® Image Acquisition and Analysis Software, the Digital Confocal* Option provides 2D on-…
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Flyer
Assay kits for the ImageXpress Pico system
Assay kits for the ImageXpress Pico system
These assay kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and apoptosis events using mammalian cells. The kits employ a no-w…
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Flyer
ImageXpress Pico Automated Cell Imaging System
ImageXpress Pico Automated Cell Imaging System
Replace tedious manual microscope manipulations with the fully automated ImageXpress® Pico Automated Cell Imaging System. Simply place samples into the system and follow the icon-driven…
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Brochure
Imaging Solutions to Answer Your Biological Questions
Imaging Solutions to Answer Your Biological Questions
Learn about our imaging family and which solution is best suited to answer your biological questions.
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Scientific Poster
Multiplexed automated imaging assays for compound testing using iPSC-derived cells
Multiplexed automated imaging assays for compound testing using iPSC-derived cells
There is an increased need for expanding variety and complexity of cell-based biologic research and drug discovery.
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Scientific Poster
Live-cell assays measuring complex compound effects in real time
Live-cell assays measuring complex compound effects in real time
Monitor cell responses in real time to acquire important qunatitative insights
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Scientific Poster
A novel high-throughput solution for screening drugs with a light-crosslinked VersaGel 3D tumor culture model and automated high-content imaging
A novel high-throughput solution for screening drugs with a light-crosslinked VersaGel 3D tumor culture model and automated high-content imaging
Screen models that mimic in vivo tumor microenvironment using novel 3D cell culture platform, VersaGel and Symphony
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Scientific Poster
Measure cell migration using automated cell imaging and analysis
Measure cell migration using automated cell imaging and analysis
Effectively image and analyze cell migration using a scratch assay or a dissolvable biocompatible gel
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Scientific Poster
Multiplexed automated imaging assays for compound testing using iPSC-derived cell models
Multiplexed automated imaging assays for compound testing using iPSC-derived cell models
Develop functional and morphological readouts for testing effects of different compounds in a multi-parametric assay format
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Scientific Poster
Cell migration analysis using automated cell imaging
Cell migration analysis using automated cell imaging
Effectively image and analyze cell migration using a dissolvable biocompatible gel.
Videos & Webinars
Accelerate your screening with high-content and automated imaging
Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening
ImageXpress Pico Automated Imaging System Virtual Tour
Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow
Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging
Automated Imaging and You – Quantitative microscopy for every lab, Powerful data for all
How to easily image slides and regions of interest with Live Preview on the ImageXpress Pico
Standard Widefield Imaging
ImageXpress Pico Automated Cell Imaging System – Feature Update
A Product Manager's Tour of the ImageXpress Pico
Configuring environmental control settings on the ImageXpress Pico
How to perform a z-stack image acquisition using CellReporterXpress
Cellular images to results in minutes with automated imaging
Setting up acquisition and analysis on the ImageXpress Pico
Transmitted light cell scoring on the ImageXpress Pico
Reviewing completed experiments on the ImageXpress Pico
ImageXpress Pico Automated Cell Imaging System-Interactive Demo
ImageXpress Pico Automated Cell Imaging System
Related Products & Services of ImageXpress Pico Automated Cell Imaging System
Featured Applications
Apoptosis Analysis
Apoptosis is a process of programmed cell death important to a number of biological processes…
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug…
Digital Confocal
The ability of cells to adapt to their environment is mediated by highly controlled signaling…
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy.…
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological…
Customer Breakthrough
SUCCESS STORY