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Digital microscopy with automated brightfield, fluorescence, and Digital Confocal imaging

The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so you can start running experiments quickly. With features such as Digital Confocal* 2D on-the-fly deconvolution, Live Preview, and multi-wavelength cell scoring, the ImageXpress Pico offers you the ability to advance your discoveries in a small, affordable imager.

  • Get started quickly

    Get started quickly

    With the icon-driven, user-friendly CellReporterXpress® Image Acquisition and Analysis Software, your entire lab can streamline their digital microscopy. Start capturing and analyzing images with minimal training.

  • Do more than cell counting

    Do more than cell counting

    Expand your assays with over 25 preconfigured templates optimized for many cell-based experiments including apoptosis, mitochondrial evaluation, 3D cell models, live cell/timelapse, multiwavelength cell scoring, and neurite tracing.

  • Automate imaging affordably

    Automate imaging affordably

    Alleviate the hassle of going to the core lab to run your samples. The system’s lab-friendly price allows researchers to afford the convenience of automated imaging and analysis on their lab bench. With options like Digital Confocal, environmental control and z-stack acquisition, the system can be ordered to fit your research.

ImageXpress Pico Automated Cell Imaging System – Feature Update


  • Multiple imaging modes

    Multiple imaging modes

    The ImageXpress Pico system offers objectives ranging from 4x to 63x and can operate in colorimetric, brightfield, fluorescence, or Digital Confocal 2D on-the-fly deconvolution imaging modes.

  • Preconfigured analysis protocols

    Preconfigured analysis protocols

    Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis. With features like the click-to-find tool, analysis parameters can be optimized by simply clicking on a few cells that match specific criteria.

  • Plate-to-individual cell view

    Plate-to-individual cell view

    Data can be visualized at multiple levels from plate overview to individual cells. A wide variety of data visualization tools from the plate and cellular level empower users to learn as much as possible from their images and assays.

  • Z-stack acquisition

    Z-stack acquisition

    Generate sharper images for more accurate segmentation using z-stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection.

  • Quickly and easily identify regions of interest

    Quickly and easily identify regions of interest

    Live Preview simplifies identification of regions of interest, letting users pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort.

  • Environmental control

    Environmental control

    Multi-day, time lapse, and live cell assays can be run using the onboard environmental system with options for humidity, CO2, and O2 control. Optimized to prevent z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions.

Experience the powerful combination of the ImageXpress Pico and CellReporterXpress






Increase resolution with on-the-fly deconvolution


Significantly increase resolution and assay quality with Digital Confocal 2D on-the-fly deconvolution. The Digital Confocal Option restores light to its original point of origin, allowing you to decrease exposure time and improve statistical significance of your observations. Digital Confocal is seamlessly integrated into the ImageXpress Pico’s fluorescent image acquisition workflow allowing you to capture images with higher signal-to-noise data for more precise segmentation and analysis.

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*ImageXpress Pico Digital Confocal uses AutoQuant 2D Real Time Deconvolution

water immersion objectives

Applications of ImageXpress Pico Automated Cell Imaging System

  • Apoptosis Analysis

    Apoptosis Analysis

    Apoptosis is a process of programmed cell death important to a number of biological processes including embryonic development and normal tissue maintenance. Disruption in the regulation of apoptosis has been implicated in various diseases including cancer. Biochemical events lead to characteristic changes in cell morphology such as cell shrinkage, nuclear fragmentation, chromatin condensation, and mRNA decay, and ultimately cell death. Apoptosis can be initiated via the intrinsic or extrinsic pathways, and both pathways induce cell death by activating caspase enzymes

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    Cancer Research

    Cancer Research

    Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.

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  • Cardiomyocytes


    Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.

    Cell Counting

    Cell Proliferation

    Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division have a need to assess the number or density of cells in a well. Automated imaging can greatly speed up the cell counting process while reducing manual labor and human errors. Cells can be counted using a variety of methods, such as label-free cell counting under transmitted light or detection of nuclear dye with fluorescent imaging.

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  • COVID-19 and Infectious Disease Research

    COVID-19 and Infectious Disease Research

    Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.

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    Digital Confocal

    Digital Confocal

    The ability of cells to adapt to their environment is mediated by highly controlled signaling pathways. In many cases, the initiation of these pathways by extracellular signals, such as hormones or cytokines, leads to the translocation of proteins from the cytoplasm to the nucleus. Once in the nucleus, these proteins can regulate the expression of target genes. Assays requiring precise identification of sub-cellular structures or molecules within different cellular compartments can benefit from the ability of image deconvolution to improve identification of the specific fluorescence signal against potentially high background caused by out-of-focus light. The ImageXpress Pico system with Digital Confocal 2D on-the-fly deconvolution is capable of measuring the translocation of proteins from the cytoplasm to the nucleus, like NF-κB translocation upon treatment with TNF-α. The enhancement in image resolution and maintenance of quantitative information from the image deconvolution leads to greater statistical significance in the analysis of nuclear translocation.

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  • Live Cell Imaging

    Live Cell Imaging

    Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.

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    Neurite Outgrowth / Neurite Tracing

    Neurite Outgrowth

    Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.

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  • Stem Cell Research

    Stem Cell Research

    Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress system has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types.

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Specifications of ImageXpress Pico Automated Cell Imaging System

Resources of ImageXpress Pico Automated Cell Imaging System

Videos & Webinars
ImageXpress Pico Environmental Control Installation

How to install the ImageXpress Pico Environmental Control System

Accelerate your Screening with Automated Imaging

Accelerate your screening with high-content and automated imaging

Microplate based Detection

Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening

ImageXpress Pico Automated Imaging

ImageXpress Pico Automated Imaging System Virtual Tour

Magnetic 3D

Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow

Developing High Throughput Organ on a Chip Tissue

Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging

Automated imaging
Automated imaging

Automated Imaging and You – Quantitative microscopy for every lab, Powerful data for all

Preview on Imagexpress Pico

How to easily image slides and regions of interest with Live Preview on the ImageXpress Pico

Standard Widefield Imaging

ImageXpress Pico Automated Cell Imaging System – Feature Update

Product Manager Interview

A Product Manager's Tour of the ImageXpress Pico

Environmental control settings in ImageXpress Pico

Configuring environmental control settings on the ImageXpress Pico

z-stack image acquisition using CellReporterXpress

How to perform a z-stack image acquisition using CellReporterXpress

ImageXpress Pico System

Cellular images to results in minutes with automated imaging

Setting up acquisition and analysis

Setting up acquisition and analysis on the ImageXpress Pico

Transmitted light cell scoring

Transmitted light cell scoring on the ImageXpress Pico

Reviewing Experiments on ImageXpress Pico

Reviewing completed experiments on the ImageXpress Pico

ImageXpress Pico Automated Cell Imaging System-Interactive Demo

ImageXpress Pico Automated Cell Imaging System

ImageXpress Pico Automated Cell Imaging System

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