ImageXpress Micro 4 High-Content Imaging System
Configurable, high-throughput widefield imaging solution for an incredible
range of assays
Widefield imaging for basic research through to high-content screening
The ImageXpress® Micro 4 High-Content Imaging System is a high-throughput, widefield imager that can acquire images of whole organisms and cellular or intracellular events. It features proprietary fast frame rate technology, allowing for the capture of fast biological processes such as calcium oscillations in cardiomyocytes. It is highly configurable and adaptable to changing research needs. Available options include confocal, brightfield, phase contrast, liquid handing, and environmental control.
Features
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Multiple imaging modes
The system offers fluorescence, widefield, colorimetric, phase contrast, and brightfield label-free imaging.
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Field upgradeable to confocal
The system can be upgraded to include the proprietary AgileOptix™ technology for accurate 3D volumetric analyses.
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Wide dynamic range
Low and high intensity signal quantification in a single image with >3 log dynamic range intensity detection.
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High speed image acquisition
Using 4x magnification with a 1536-well plate, 225K wells per day can be acquired. Greater than a million wells per week can be imaged.
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Optional environmental control
Temperature, humidity, and CO2 control for multi-day live-cell imaging or fast kinetic studies are available.
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Large magnification range
Air and oil immersion objectives ranging from 1x to 100x are available for installation into the 4-position objective changer.
MetaXpress high-content image aquisition and analysis software
- Meet high throughput requirements with a scalable, streamlined workflow
- Adapt your analysis tools to tackle your toughest problems, including 3D analysis
- Schedule automatic data transfer between third-party hardware sources and secure database
- Set up hundreds of routinely used HCS assays using MetaXpress software modules
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eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
-
Brochure
MetaXpress High-Content Image Acquisition and Analysis Software
MetaXpress High-Content Image Acquisition and Analysis Software
Capture high-resolution images, recognize and segment objects, calculate parameters, and then translate your information into meaningful data. At Molecular Devices, we’re inspired by your cr…
Applications of ImageXpress Micro 4 High-Content Imaging System
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3D Cell Models
The development and integration of three-dimensional (3D) assay models are becoming popular to drive translational biology. Our ImageXpress® Micro Confocal High-Content Imaging System allows superior visualization of thick samples by minimizing background fluorescence and increasing sharpness, resulting in accurate image segmentation. Increase the biological relevance of your screening assays with a seamless integration between acquisition and analysis of cells in a 3D space to yield volume, intensity, and distance measurements.
3D Cell Models
The development and integration of three-dimensional (3D) assay models are becoming popular to drive translational biology. Our ImageXpress® Micro Confocal High-Content Imaging System allows superior visualization of thick samples by minimizing background fluorescence and increasing sharpness, resulting in accurate image segmentation. Increase the biological relevance of your screening assays with a seamless integration between acquisition and analysis of cells in a 3D space to yield volume, intensity, and distance measurements.
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.
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Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.
Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division rely on the assessment of the number or density of cells in a well.
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division rely on the assessment of the number or density of cells in a well.
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Cell Migration Assays
The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell timelapse imaging.
Cell Migration Assays
The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell timelapse imaging.
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.
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Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
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Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress system has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types.
Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress system has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types.
Toxicity Screening
Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.
Toxicity Screening
Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.
Resources of ImageXpress Micro 4 High-Content Imaging System
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eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
-
Brochure
MetaXpress High-Content Image Acquisition and Analysis Software
MetaXpress High-Content Image Acquisition and Analysis Software
Capture high-resolution images, recognize and segment objects, calculate parameters, and then translate your information into meaningful data. At Molecular Devices, we’re inspired by…
-
Brochure
3D Image Analysis Module
3D Image Analysis Module
Complex assays and three-dimensional cell models better represent tissue biology and cell interactions, making them more relevant for many toxicity and drug screening assays. The abi…
Citations
Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays
Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays
We present here methods for assessing hepatotoxicity by high content imaging and image analysis. The assays focus on the characterization of toxic effects using a variety of phenotyp…
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eBook
High-content imaging for diverse 3D cell culture models
High-content imaging for diverse 3D cell culture models
Investigate diverse 3D models and resolve common challenges experienced in 3D cell culture assays.
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Citations
Haemagglutinin stability was not the primary cause of the reduced effectiveness of live attenuated influenza vaccine against A/H1N1pdm09 viruses in the 2013–2014 and 2015–2016 seasons
Haemagglutinin stability was not the primary cause of the reduced effectiveness of live attenuated influenza vaccine against A/H1N1pdm09 viruses in the 2013–2014 and 2015–2016 seasons
In these studies, we confirm that the A/CA09 LAIV virus is more temperature and pH unstable than historical LAIV influenza A viruses with proven VE. These remain a possible contribut…
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Citations
Liver Fibrosis and Inflammation under the Control of ERK2
Liver Fibrosis and Inflammation under the Control of ERK2
Chronic liver injury could lead the formation of liver fibrosis, eventually some would develop to hepatocellular carcinoma (HCC), one of the leading malignancies worldwide. The aim o…
Scientific Poster
High-throughput single-cell contractility measurements using FLECS with ImageXpress Micro
High-throughput single-cell contractility measurements using FLECS with ImageXpress Micro
Track contractility of each single cell within each population over long experimental times.
Application Note
High-throughput screening of single-cell contractile force using FLECS Technology
High-throughput screening of single-cell contractile force using FLECS Technology
The ability of mammalian cells to generate mechanical forces—to push, pull, or squeeze—is an intrinsic capability that is used by cells both individually and together as tissue to…
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Scientific Poster
Measure cell migration using automated cell imaging and analysis
Measure cell migration using automated cell imaging and analysis
Effectively image and analyze cell migration using a scratch assay or a dissolvable biocompatible gel
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Application Note
Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
Multiplexed automated imaging assay for cardiotoxic compounds using the ImageXpress Pico system
Develop functional and morphological readouts for testing effects of different compounds in multi-parametric assays using iPSC-derived cardiomyocytes
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eBook
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
This new eBook discusses early drug safety testing using HTS iPSC-derived cells with imaging. Download it now!
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eBook
Cellular Imaging Insights
Cellular Imaging Insights
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
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eBook
Acquire and analyze 3D images like a pro
Acquire and analyze 3D images like a pro
There has been significant progress in the development of 3D models and techniques during the last few years. Methods include biodegradable scaffolds, organ-on-a chip structures, or…
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Scientific Poster
High-Throughput Assays for Characterizing Viability and Morphology of 3D Cancer Spheroid Cultures
High-Throughput Assays for Characterizing Viability and Morphology of 3D Cancer Spheroid Cultures
Three-dimensional (3D) in vitro models span the gap between two-dimensional cell cultures and whole-animal systems. By mimicking features of the in vivo environment and taking advant…
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Application Note
Evaluating cell cycle inhibitors using a live cell assay
Evaluating cell cycle inhibitors using a live cell assay
Monitoring treatment effects on the cell cycle is relevant to progressing oncology research and drug discovery. High-content screening assays using live cells have been developed to…
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Application Note
Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
Growing concerns about the increased prevalence of untested chemicals in the environment has created a pressing need to develop reliable and efficient screening tools to identify…
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Brochure
Imaging Solutions to Answer Your Biological Questions
Imaging Solutions to Answer Your Biological Questions
Learn about our imaging family and which solution is best suited to answer your biological questions.
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Scientific Poster
Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Development of predictive in vitro assays for early toxicity evaluation is extremely important for improving the drug development process and reducing drug attrition rates during cli…
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Scientific Poster
Novel High Content Stem Cell-based Assays for Screening
Novel High Content Stem Cell-based Assays for Screening
The biopharma industry is continuing to adapt more cell-based assays for primary and secondary screening because of higher biological relevance and increased value of information.…
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Application Note
High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
In recent years, there has been significant progress in development of in vitro aggregates of tumor cells for use as models for in vivo tissue environments. When seeded into a well of…
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Application Note
Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
Cardiotoxicity remains a primary cause for drug attrition during clinical trials and post-marketing. While it is believed that a significant percentage of cardiovascular diseases are…
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Application Note
Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
Cell viability assays are utilized in a wide variety of research areas ranging from examining the mechanisms of cell death and cell proliferation to evaluating the effects of cytotoxic…
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Application Note
Live cell kinetics assay utilizing the ImageXpress Micro System
Live cell kinetics assay utilizing the ImageXpress Micro System
The ImageXpress® Micro Confocal/ XLS Widefield High-Content Imaging System from Molecular Devices includes optional Fluidics and Environmental Control modules, enabling single-channel…
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Application Note
Assaying cardiotoxicity with ImageXpress high-content screening systems
Assaying cardiotoxicity with ImageXpress high-content screening systems
The discovery and development of novel drugs is a difficult and expensive process due to lack of tools and techniques to accurately reproduce the human physiological response in the…
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Application Note
Visualize subcellular vesicles to quantitate autophagy in neuronal cells
Visualize subcellular vesicles to quantitate autophagy in neuronal cells
Autophagy is an intracellular catabolic process that sequesters and degrades proteins and organelles that have either been recognized as faulty or are simply no longer needed by the…
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Application Note
Increase sensitivity in no-wash assays using confocal imaging
Increase sensitivity in no-wash assays using confocal imaging
Screening of hybridoma supernatants for antibodies directed against cell-surface antigens is an important step in the discovery and development of antibodies for vaccines or…
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Application Note
High-throughput imaging assays using zebrafish, a model organism for human disease
High-throughput imaging assays using zebrafish, a model organism for human disease
Recently, zebrafish-based screening has gained favor as an alternative to mammalian screening due to cost, throughput and reduced ethical concerns. Zebrafish are a useful model for…
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Application Note
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis is an important mechanism signaling programmed death of cells in normal processes such as embryonic development, as well as in diseases including cancer and neurodegenerative…
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Application Note
High-content screening of neuronal toxicity using iPSC-derived human neurons
High-content screening of neuronal toxicity using iPSC-derived human neurons
The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. Neurotoxicity can cause temporary or…
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Application Note
Intelligent time-lapse imaging to enhance live cell assay development
Intelligent time-lapse imaging to enhance live cell assay development
The ability to monitor responses in living cells over a specific period of time offers cell biologists several key advantages for assay development. For routine cellbased screening,…
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Application Note
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus highly predictive assays for safety and efficacy testing are crucial for improving drug…
Data Sheet
NEW ImageXpress Micro 4 High-Content Imaging System
NEW ImageXpress Micro 4 High-Content Imaging System
The ImageXpress® Micro 4 High-Content Imaging System represents the culmination of four generations of imaging expertise. The latest agile design allows you to boost your research wi…
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Scientific Poster
High-Throughput High-Content Screening for Developmental Neurotoxicity in Human iPSC-Derived Neurons
High-Throughput High-Content Screening for Developmental Neurotoxicity in Human iPSC-Derived Neurons
There is an increased need to develop reliable and efficient screening tools to identify chemicals with the potential to adversely affect neurological development. Human iPSC-derived…
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Scientific Poster
Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
Read more about cell based assays for measuring the impact of pharmacological compounds on the rate of beating cardiomyocytes with different assay platforms.
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Scientific Poster
Morphometric Characterization of Cancer Spheroid Cultures Using Confocal Imaging and 3D Image Analysis
Morphometric Characterization of Cancer Spheroid Cultures Using Confocal Imaging and 3D Image Analysis
There is an emerging interest in using three-dimensional (3D) organoid cultures for modeling tissue biology and cancer. Development of higher throughput assays to quantify phenotypic…
Unleash the power of cell painting
Transitioning high-content assays to 3D: Scientific opportunities and imaging challenges
Accelerate your screening with high-content and automated imaging
Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening
Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow
LabTube Meets Molecular Devices & MIMETAS, Susan Murphy & Sebastiaan Trietsch
Plate annotation and curve fitting in MetaXpress
Quickstart Guide: Plate acquisition on the ImageXpress Micro Confocal using MetaXpress
Quickstart Guide: Review images on the ImageXpress Micro Confocal using MetaXpress
Basic workflow from image acquisition to analysis on the ImageXpress Micro Confocal using MetaXpress
Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging
3D Imaging of Cancer Spheroids
Whole Organism Imaging
High-Content Screening for Identifying miRNAs
Identification of Selective Inhibitors of the STAT3 Signaling Pathway
Oliver Kepp and Jayne Hesley - Hallmarks of Cancer - Detect and Quantify Cell Death Signatures with High Content Imaging
ImageXpress Micro 4 Imaging System
Multidimensional High-Throughput Imaging with the New ImageXpress Micro Confocal System
Pushing the Boundaries of High Content Screening
Preparing Assays for Genome-wide RNAi Screens Using High Content Microscopy
Multiplexed High Content Hepatoxicity Assays Using iPSC-Derived Hepatocytes
High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 Tissue Models
Simple and Flexible High Content Imaging Enabling Quantitation of Complex Biological Events
Contemporary Automation and High Content Imaging Tools for Screening Stem Cell-Derived Cardiomyocytes
Implement High-Throughput 3D Image Analysis for Samples from Subcellular Structures to Spheroids
Live Cell Imaging to Investigate the Regulation of Cell Division Timing
Using High-throughput RNA Screening to Identify Host Factors That Impact Viral Infection
Application of HCA Tools for Antibody Drug Discovery
Setting up 3D Spheroid Assays Using High-Content Imaging
Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery
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Citations
Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays
We present here methods for assessing hepatotoxicity by high content imaging and image analysis. The assays focus on the characterization of toxic…
-
Citations
Haemagglutinin stability was not the primary cause of the reduced effectiveness of live attenuated influenza vaccine against A/H1N1pdm09 viruses in the 2013–2014 and 2015–2016 seasons
In these studies, we confirm that the A/CA09 LAIV virus is more temperature and pH unstable than historical LAIV influenza A viruses with proven VE…
-
Citations
Liver Fibrosis and Inflammation under the Control of ERK2
Chronic liver injury could lead the formation of liver fibrosis, eventually some would develop to hepatocellular carcinoma (HCC), one of the leadin…
Related Products & Services of ImageXpress Micro 4 High-Content Imaging System
Expand your research using flexible imaging solutions
Molecular Devices provides flexible options for the ImageXpress Micro 4 High-Content Imaging System to meet your research needs and to easily capture images from different sample formats, including hanging drops and in round or flat bottom plates, for monitoring cell health kinetics under environmental control, and more. With over 30+ years of imaging expertise, we can help you select the right options to ensure the best images for your assay.
ENVIRONMENTAL CONTROL
Environmental control maintains temperature and humidity levels while minimizing evaporation for multi-day, live cell, time-lapse imaging.
TRANSMITTED LIGHT TOWER
Our transmitted light tower enables acquisition of high contrast images for unstained cells which can be easily viewed or separated from background.
ON-BOARD ROBOTIC
FLUIDICS
Integrated fluidics automates assay workflows which require compound addition, well washing, and media exchange.
Implement a solution that works for you
Molecular Devices can successfully tailor the ImageXpress Micro 4 High-Content Imaging System to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support.
Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms
Featured Applications
3D Cell Models
The development and integration of three-dimensional (3D) assay models are becoming popular to…
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly…
Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological…
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug…
Cell Migration Assays
The movement or migration of cells is often measured in vitro to elucidate the mechanisms of…
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line…
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy.…
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological…
Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a…
Toxicity Screening
Screening for off-target or toxic effects is very important during the development of new drugs and…
Customer Breakthrough
SUCCESS STORY
Forcyte Biotechnologies uses the ImageXpress Micro 4 system to help execute mechano-medicine discovery screens
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
Maximize flexibility in application workflow and experimental design
Contact our specialist today to discuss how to implement a solution that works for you