ImageXpress Micro 4 High-Content Imaging System
Configurable, high-throughput widefield imaging solution for an incredible
range of assays
Widefield imaging for basic research through to high-content screening
The ImageXpress® Micro 4 High-Content Imaging System is a high-throughput, widefield imager that can acquire images of whole organisms and cellular or intracellular events. It features proprietary fast frame rate technology, allowing for the capture of fast biological processes such as calcium oscillations in cardiomyocytes. It is highly configurable and adaptable to changing research needs. Available options include confocal, brightfield, phase contrast, liquid handing, and environmental control.
Features
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Multiple imaging modes
The system offers fluorescence, widefield, colorimetric, phase contrast, and brightfield label-free imaging.
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Upgradeable to confocal
The system can be upgraded to include the proprietary AgileOptix™ technology for accurate 3D volumetric analyses.
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Wide dynamic range
Low and high intensity signal quantification in a single image with >3 log dynamic range intensity detection.
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High speed image acquisition
Using 4x magnification with a 1536-well plate, 225K wells per day can be acquired. Greater than a million wells per week can be imaged.
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Optional environmental control
Temperature, humidity, and CO2 control for multi-day live-cell imaging or fast kinetic studies are available.
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Large magnification range
Air and oil immersion objectives ranging from 1x to 100x are available for installation into the 4-position objective changer.
MetaXpress high-content image aquisition and analysis software
- Meet high throughput requirements with a scalable, streamlined workflow
- Adapt your analysis tools to tackle your toughest problems, including 3D analysis
- Schedule automatic data transfer between third-party hardware sources and secure database
- Set up hundreds of routinely used HCS assays using MetaXpress software modules
![3D organoids and automation of complex cell assays [Podcast]](/sites/default/files/2023-01/3d-organoids-and-automation-of-complex-cell-assays.jpg)
Applications of ImageXpress Micro 4 High-Content Imaging System
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3D Cell Models
3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics. Utilization of 3D cellular assays adds value to research and screening campaigns, spanning the translational gap between 2D cell cultures and whole-animal models. By reproducing important parameters of the in vivo environment, 3D models can provide unique insight into the behavior of stem cells and developing tissues in vitro.
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.
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Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug compound toxicity, cell proliferation, and inhibition of cell division rely on the assessment of the number or density of cells in a well.
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Cell Migration Assays
The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell timelapse imaging.
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.
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Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
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Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress system has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types.
Toxicology
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.
Resources of ImageXpress Micro 4 High-Content Imaging System
Blog
How 3D Cell Models Will Shape the Future of Drug Discovery
How 3D Cell Models Will Shape the Future of Drug Discovery
Target discovery and drug development rely heavily on 2D cell and animal models to decipher efficacy and toxic effect of drug candidates. Yet, 90% of candidates fail to make it past…
News
Molecular Devices inks deal with HUB Organoids to advance automated intestinal organoid screening technology
Molecular Devices inks deal with HUB Organoids to advance automated intestinal organoid screening technology
SAN JOSE, Calif., and UTRECHT, Netherlands, Feb. 13, 2023 – Molecular Devices, LLC., a leading provider of high-performance life science solutions, and HUB Organoids (HUB), the pioneer…
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Blog
3D organoids and automation of complex cell assays [Podcast]
3D organoids and automation of complex cell assays [Podcast]
As we enter the era of sophisticated drug discovery with gene therapy and personalized medicine, we need to be prepared to study complex diseases, assess the therapeutic effect of…
Publications
Gene editing in organoids: accounting for complexity in drug discovery
Gene editing in organoids: accounting for complexity in drug discovery
More researchers are using gene editing to build disease models that better represent human tissues' complex biology, signaling a shift away from 2D cell culture or animal models to…
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Publications
Bioprinting Automation for Drug Discovery to Be Developed by Molecular Devices and Advanced Solutions
Bioprinting Automation for Drug Discovery to Be Developed by Molecular Devices and Advanced Solutions
3DPrint.com, the leading source for actionable intelligence related to 3D printing technology and the larger AM industry, covers our collaboration…
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Publications
Improving Drug Development: Molecular Devices and Cellesce Aim to Advance the Use of Organoids
Improving Drug Development: Molecular Devices and Cellesce Aim to Advance the Use of Organoids
Following our acquisition of Cellesce Ltd, Tanya Samazan from Instrument Business Outlook dove deep into conversation with Molecular Devices President Susan Murphy and Cellesce CEO V…
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News
Molecular Devices and Advanced Solutions Life Sciences collaborate to develop 3D Biology Automation Technologies for Drug Discovery
Molecular Devices and Advanced Solutions Life Sciences collaborate to develop 3D Biology Automation Technologies for Drug Discovery
Turnkey platform integrates flexible robotic automation with high-content imaging of complex 3D cellular models, enabling high-volume organoid screening SAN JOSE, Calif., Jan. 4, 2023…
Brochure
BioAssemblyBot 400 (BAB400): more than a bioprinter
BioAssemblyBot 400 (BAB400): more than a bioprinter
The BioAssemblyBot® 400 is an intelligent robot used by life scientists to build 3D model systems with increased throughput and precision, alleviating major concerns mentioned above.…
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News
Molecular Devices adds proprietary patient-derived organoid technology with acquisition of Cellesce
Molecular Devices adds proprietary patient-derived organoid technology with acquisition of Cellesce
First-of-its-kind technology from Cellesce creates consistent patient-derived organoids for large scale drug screening Acquisition strengthens Molecular Devices’ position as a 3D…
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Publications
Overcoming ‘Analysis Paralysis’ in Cell Painting With Artificial Intelligence
Overcoming ‘Analysis Paralysis’ in Cell Painting With Artificial Intelligence
Gone are the days of only measuring single parameters in cell-based experiments. Instead, researchers should widen their focus, namely with the help of innovations like the Cell Pain…
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Publications
How cardioids can usher in the next generation of drug discovery
How cardioids can usher in the next generation of drug discovery
We recently partnered with HeartBeat.bio to automate and scale the production of cardioids — 3D cell models of the heart that can more accurately recapitulate human biology.
…
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Publications
What an innovation centre means for 3D biology
What an innovation centre means for 3D biology
In April 2021, we launched our Organoid Innovation Centre (OIC), an initiative designed to help scientists leverage the potential of 3D biology throughout the drug discovery process.…
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Blog
Innovation at Molecular Devices: Updates in Automated, High-Content Imaging
Innovation at Molecular Devices: Updates in Automated, High-Content Imaging
From customer feedback to workflow improvements The path to understanding complex biological processes and diseases is paved with a lot of challenges. As the desired level of…
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Blog
Stem cell science insights and breakthroughs presented at #ISSCR2021
Stem cell science insights and breakthroughs presented at #ISSCR2021
If you didn't get a chance to visit us at our poster sessions during ISSCR 2021, don’t fret. We've gathered all our sessions right here for you. The ISSCR Annual Meeting brought…
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Blog
Tips for running a successful live cell imaging experiment
Tips for running a successful live cell imaging experiment
There have been significant advancements in microscopy and camera technology, as well as advancements in technologies for labeling molecules of interest over the past decade. These…
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Blog
Overcome the challenges of high-content cell analysis through AI/machine learning
Overcome the challenges of high-content cell analysis through AI/machine learning
Artificial intelligence (AI) is finding its way into many aspects of modern life, from autonomous vehicles to voice-powered personal assistants, and even the creation of art. But it…
Scientific Poster
Automated 3D cell-based assays using a novel flow chip system and high-content imaging
Automated 3D cell-based assays using a novel flow chip system and high-content imaging
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translation research. Significant progress has b…
eBook
Cellular Imaging Insights
Cellular Imaging Insights
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
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Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 40 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
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Blog
Get to know our Field Applications Scientist: Kayla Hill
Get to know our Field Applications Scientist: Kayla Hill
Kayla Hill discusses the latest trends in cellular imaging We recently hosted a webinar with our Field Applications Scientist, Kayla Hill, PhD, who explored high-content analysis…
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eBook
High-content imaging for diverse 3D cell culture models
High-content imaging for diverse 3D cell culture models
Investigate diverse 3D models and resolve common challenges experienced in 3D cell culture assays.
Customer Breakthrough
Forcyte Biotechnologies uses the ImageXpress Micro 4 system to help execute mechano-medicine discovery screens
Forcyte Biotechnologies uses the ImageXpress Micro 4 system to help execute mechano-medicine discovery screens
Forcyte Biotechnologies, Inc. is a drug discovery startup performing phenotypic screening of contractile cell force as a HTS assay endpoint. The company manufactures and utilizes uni…
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Scientific Poster
High-throughput single-cell contractility measurements using FLECS with ImageXpress Micro
High-throughput single-cell contractility measurements using FLECS with ImageXpress Micro
Track contractility of each single cell within each population over long experimental times.
Application Note
High-throughput screening of single-cell contractile force using FLECS Technology
High-throughput screening of single-cell contractile force using FLECS Technology
The ability of mammalian cells to generate mechanical forces—to push, pull, or squeeze—is an intrinsic capability that is used by cells both individually and together as tissue to…
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Scientific Poster
Measure cell migration using automated cell imaging and analysis
Measure cell migration using automated cell imaging and analysis
Effectively image and analyze cell migration using a scratch assay or a dissolvable biocompatible gel
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eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
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eBook
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
This new eBook discusses early drug safety testing using HTS iPSC-derived cells with imaging. Download it now!
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Customer Breakthrough
MIMETAS uses the ImageXpress Pico and ImageXpress Micro Confocal systems to develop tissue models for their OrganoPlates®
MIMETAS uses the ImageXpress Pico and ImageXpress Micro Confocal systems to develop tissue models for their OrganoPlates®
MIMETAS offers the OrganoPlate®, a unique 3D organ-on-a-chip platform. The OrganoPlate® is a fully compatible microfluidic culture plate, enabling testing of compounds in any through…
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eBook
Acquire and analyze 3D images like a pro
Acquire and analyze 3D images like a pro
There has been significant progress in the development of 3D models and techniques during the last few years. Methods include biodegradable scaffolds, organ-on-a chip structures, or…
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Scientific Poster
High-Throughput Assays for Characterizing Viability and Morphology of 3D Cancer Spheroid Cultures
High-Throughput Assays for Characterizing Viability and Morphology of 3D Cancer Spheroid Cultures
Three-dimensional (3D) in vitro models span the gap between two-dimensional cell cultures and whole-animal systems. By mimicking features of the in vivo environment and taking advant…
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Application Note
Evaluating cell cycle inhibitors using a live cell assay
Evaluating cell cycle inhibitors using a live cell assay
Monitoring treatment effects on the cell cycle is relevant to progressing oncology research and drug discovery. High-content screening assays using live cells have been developed to…
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Application Note
Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
Growing concerns about the increased prevalence of untested chemicals in the environment has created a pressing need to develop reliable and efficient screening tools to identify…
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Brochure
Imaging Solutions to Answer Your Biological Questions
Imaging Solutions to Answer Your Biological Questions
Learn about our imaging family and which solution is best suited to answer your biological questions.
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Brochure
MetaXpress High-Content Image Acquisition and Analysis Software
MetaXpress High-Content Image Acquisition and Analysis Software
Capture high-resolution images, recognize and segment objects, calculate parameters, and then translate your information into meaningful data. At Molecular Devices, we’re inspired by…
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Scientific Poster
Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Development of predictive in vitro assays for early toxicity evaluation is extremely important for improving the drug development process and reducing drug attrition rates during cli…
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Scientific Poster
Novel High Content Stem Cell-based Assays for Screening
Novel High Content Stem Cell-based Assays for Screening
The biopharma industry is continuing to adapt more cell-based assays for primary and secondary screening because of higher biological relevance and increased value of information.…
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Customer Breakthrough
SENS Research Foundation uses the ImageXpress Micro Widefield system to speed up cancer research 20 times
SENS Research Foundation uses the ImageXpress Micro Widefield system to speed up cancer research 20 times
The Oncology team at the SENS Research Foundation seeks to uncover the role of telomere lengthening in cancer development. The Alternative Lengthening of Telomeres (ALT) pathway acco…
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Application Note
High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
In recent years, there has been significant progress in development of in vitro aggregates of tumor cells for use as models for in vivo tissue environments. When seeded into a well of…
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Application Note
Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
Cardiotoxicity remains a primary cause for drug attrition during clinical trials and post-marketing. While it is believed that a significant percentage of cardiovascular diseases are…
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Application Note
Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
Cell viability assays are utilized in a wide variety of research areas ranging from examining the mechanisms of cell death and cell proliferation to evaluating the effects of cytotoxic…
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Application Note
Live cell kinetics assay utilizing the ImageXpress Micro System
Live cell kinetics assay utilizing the ImageXpress Micro System
The ImageXpress® Micro Confocal/ XLS Widefield High-Content Imaging System from Molecular Devices includes optional Fluidics and Environmental Control modules, enabling single-channel…
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Application Note
Assaying cardiotoxicity with ImageXpress high-content screening systems
Assaying cardiotoxicity with ImageXpress high-content screening systems
The discovery and development of novel drugs is a difficult and expensive process due to lack of tools and techniques to accurately reproduce the human physiological response in the…
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Application Note
Visualize subcellular vesicles to quantitate autophagy in neuronal cells
Visualize subcellular vesicles to quantitate autophagy in neuronal cells
Autophagy is an intracellular catabolic process that sequesters and degrades proteins and organelles that have either been recognized as faulty or are simply no longer needed by the…
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Application Note
Increase sensitivity in no-wash assays using confocal imaging
Increase sensitivity in no-wash assays using confocal imaging
Screening of hybridoma supernatants for antibodies directed against cell-surface antigens is an important step in the discovery and development of antibodies for vaccines or…
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Application Note
High-throughput imaging assays using zebrafish, a model organism for human disease
High-throughput imaging assays using zebrafish, a model organism for human disease
Recently, zebrafish-based screening has gained favor as an alternative to mammalian screening due to cost, throughput and reduced ethical concerns. Zebrafish are a useful model for…
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Application Note
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis is an important mechanism signaling programmed death of cells in normal processes such as embryonic development, as well as in diseases including cancer and neurodegenerative…
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Application Note
High-content screening of neuronal toxicity using iPSC-derived human neurons
High-content screening of neuronal toxicity using iPSC-derived human neurons
The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. Neurotoxicity can cause temporary or…
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Application Note
Intelligent time-lapse imaging to enhance live cell assay development
Intelligent time-lapse imaging to enhance live cell assay development
The ability to monitor responses in living cells over a specific period of time offers cell biologists several key advantages for assay development. For routine cellbased screening,…
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Application Note
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus highly predictive assays for safety and efficacy testing are crucial for improving drug…
Data Sheet
NEW ImageXpress Micro 4 High-Content Imaging System
NEW ImageXpress Micro 4 High-Content Imaging System
The ImageXpress® Micro 4 High-Content Imaging System represents the culmination of four generations of imaging expertise. The latest agile design allows you to boost your research wi…
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Scientific Poster
High-Throughput High-Content Screening for Developmental Neurotoxicity in Human iPSC-Derived Neurons
High-Throughput High-Content Screening for Developmental Neurotoxicity in Human iPSC-Derived Neurons
There is an increased need to develop reliable and efficient screening tools to identify chemicals with the potential to adversely affect neurological development. Human iPSC-derived…
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Scientific Poster
Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
Read more about cell based assays for measuring the impact of pharmacological compounds on the rate of beating cardiomyocytes with different assay platforms.
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Scientific Poster
Morphometric Characterization of Cancer Spheroid Cultures Using Confocal Imaging and 3D Image Analysis
Morphometric Characterization of Cancer Spheroid Cultures Using Confocal Imaging and 3D Image Analysis
There is an emerging interest in using three-dimensional (3D) organoid cultures for modeling tissue biology and cancer. Development of higher throughput assays to quantify phenotypic…
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Brochure
3D Image Analysis Module
3D Image Analysis Module
Complex assays and three-dimensional cell models better represent tissue biology and cell interactions, making them more relevant for many toxicity and drug screening assays. The abi…
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Publications
Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes show promise for screening during early drug development. Here, we tested a hypothesis that in vitro assessment of m…
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Publications
Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells
Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells
A large percentage of drugs fail in clinical studies due to cardiac toxicity; thus, development of sensitive in vitro assays that can evaluate potential adverse effects on cardiomyoc…
Videos & Webinars
Immunology and Vaccine Development Workflow
Hybridoma Workflow
Unleash the power of Cell Painting
Transitioning high-content assays to 3D: Scientific opportunities and imaging challenges
Accelerate your screening with high-content and automated imaging
Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening
Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow
LabTube Meets Molecular Devices & MIMETAS, Susan Murphy & Sebastiaan Trietsch
Plate annotation and curve fitting in MetaXpress
Quickstart Guide: Plate acquisition on the ImageXpress Micro Confocal using MetaXpress
Quickstart Guide: Review images on the ImageXpress Micro Confocal using MetaXpress
Basic workflow from image acquisition to analysis on the ImageXpress Micro Confocal using MetaXpress
Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging
3D Imaging of Cancer Spheroids
Whole Organism Imaging
High-Content Screening for Identifying miRNAs
Identification of Selective Inhibitors of the STAT3 Signaling Pathway
Oliver Kepp and Jayne Hesley - Hallmarks of Cancer - Detect and Quantify Cell Death Signatures with High Content Imaging
ImageXpress Micro 4 Imaging System
Multidimensional High-Throughput Imaging with the New ImageXpress Micro Confocal System
Pushing the Boundaries of High Content Screening
Preparing Assays for Genome-wide RNAi Screens Using High Content Microscopy
Multiplexed High Content Hepatoxicity Assays Using iPSC-Derived Hepatocytes
High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 Tissue Models
Simple and Flexible High Content Imaging Enabling Quantitation of Complex Biological Events
Contemporary Automation and High Content Imaging Tools for Screening Stem Cell-Derived Cardiomyocytes
Implement High-Throughput 3D Image Analysis for Samples from Subcellular Structures to Spheroids
Live Cell Imaging to Investigate the Regulation of Cell Division Timing
Using High-throughput RNA Screening to Identify Host Factors That Impact Viral Infection
Application of HCA Tools for Antibody Drug Discovery
Setting up 3D Spheroid Assays Using High-Content Imaging
Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery
Number of Citations*: 80
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Dated: Oct 30, 2020Publication Name: High Content ScreeningDetermination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays
We present here methods for assessing hepatotoxicity by high content imaging and image analysis. The assays focus on the characterization of toxic effects using a variety of phenotypic endpoint readouts. Multi-parametric automated image analysis is used in the protocols to increase assay sensitivity and provide important information about possible… View moreWe present here methods for assessing hepatotoxicity by high content imaging and image analysis. The assays focus on the characterization of toxic effects using a variety of phenotypic endpoint readouts. Multi-parametric automated image analysis is used in the protocols to increase assay sensitivity and provide important information about possible in vitro toxicity mechanisms. iPSC-derived hepatocytes were used as a model for the hepatotoxicity assays, but the methods would also be suitable for other liver toxicity cell models. The methods contain detailed step-by-step descriptions of the cell treatment, staining, image acquisition, and image analysis.
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Dated: May 01, 2020Publication Name: Han Xiao, Yue Wang, Xiaojing Jia, Lei Yang, Xiaoning Wang, Xuan Guo, Zhaobin Zhang
Tris(4-hydroxyphenyl)ethane (THPE), a trisphenol compound, is antiestrogenic and can retard uterine development in CD-1 mice
Tris (4-hydroxyphenyl)ethane (THPE), a trisphenol compound widely used as a branching agent and raw material in plastics, adhesives, and coatings is rarely regarded with concern. However, inspection of in vitro data suggests that THPE is an antagonist of estrogen receptors (ERs). Accordingly, we aimed to evaluate the antiestrogenicity of THPE in… View moreTris (4-hydroxyphenyl)ethane (THPE), a trisphenol compound widely used as a branching agent and raw material in plastics, adhesives, and coatings is rarely regarded with concern. However, inspection of in vitro data suggests that THPE is an antagonist of estrogen receptors (ERs). Accordingly, we aimed to evaluate the antiestrogenicity of THPE in vivo and tested its effect via oral gavage on pubertal development in female CD-1 mice. Using uterotrophic assays, we found that THPE either singly, or combined with 17β-estradiol (E2) (400 μg/kg bw/day) suppressed the uterine weights at low doses (0.1, 0.3, and 1 mg/kg bw/day) in 3-day treatment of weaning mice. When mice were treated with THPE during adolescence (for 10 days beginning on postnatal day 24), their uterine development was significantly retarded at doses of at least 0.1 mg/kg bw/day, manifest as decreased uterine weight, atrophic endometrial stromal cells and thinner columnar epithelial cells. Transcriptome analyses of uteri demonstrated that estrogen-responsive genes were significantly downregulated by THPE. Molecular docking shows that THPE fits well into the antagonist pocket of human ERα. These results indicate that THPE possesses strong antiestrogenicity in vivo and can disrupt normal female development in mice at very low dosages.
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Dated: Oct 04, 2018Publication Name: British Journal of Anaesthesia
Lidocaine inhibits cytoskeletal remodelling and human breast cancer cell migration
The metastatic potential of breast cancer cells has been strongly associated with overexpression of the chemokine CXCL12 and the activity of its receptor CXCR4. Lidocaine, a local anaesthetic that can be used during breast cancer excision, inhibits the growth, invasion, and migration of cancer cells. We therefore investigated, in a breast cancer… View moreThe metastatic potential of breast cancer cells has been strongly associated with overexpression of the chemokine CXCL12 and the activity of its receptor CXCR4. Lidocaine, a local anaesthetic that can be used during breast cancer excision, inhibits the growth, invasion, and migration of cancer cells. We therefore investigated, in a breast cancer cell line, whether lidocaine can modulate CXCL12-induced responses.
Contributors: G.D'Agostino, A.Saporito, V.Cecchinato, Y.Silvestri, A.Borgeat, L.Anselmi, M.Uguccioni
Go to article
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Cellular Imaging Systems
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Transform your incredible images into captivating results
Expand your research using flexible imaging solutions
Molecular Devices provides flexible options for the ImageXpress Micro 4 High-Content Imaging System to meet your research needs and to easily capture images from different sample formats, including hanging drops and in round or flat bottom plates, for monitoring cell health kinetics under environmental control, and more.
ENVIRONMENTAL CONTROL
Environmental control maintains temperature and humidity levels while minimizing evaporation for multi-day, live cell, time-lapse imaging.
TRANSMITTED LIGHT TOWER
Our transmitted light tower enables acquisition of high contrast images for unstained cells which can be easily viewed or separated from background.
ON-BOARD ROBOTIC
FLUIDICS
Integrated fluidics automates assay workflows which require compound addition, well washing, and media exchange.
Implement a solution that works for you
Molecular Devices can successfully tailor the ImageXpress Micro 4 High-Content Imaging System to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support.
Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms
Featured Applications
3D Cell Models
3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including…
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly…
Cardiomyocytes
Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological…
Cell Counting
Cell counting is fundamental and critical to numerous biological experiments. Assays such as drug…
Cell Migration Assays
The movement or migration of cells is often measured in vitro to elucidate the mechanisms of…
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line…
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy.…
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological…
Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a…
Toxicology
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It…
Customer Breakthrough
SUCCESS STORY
Forcyte Biotechnologies uses the ImageXpress Micro 4 system to help execute mechano-medicine discovery screens
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…