Widefield imaging for basic research through to high-content screening

The ImageXpress® Micro 4 High-Content Imaging System is a high-throughput, widefield imager that can acquire images of whole organisms and cellular or intracellular events. It features proprietary fast frame rate technology, allowing for the capture of fast biological processes such as calcium oscillations in cardiomyocytes. It is highly configurable and adaptable to changing research needs. Available options include confocal, brightfield, phase contrast, liquid handing, and environmental control.

  • Expandable

    Support many cellular assays

    Image an incredible range of assays with various user-changeable filter cubes, 1-100X objectives, environmental control, fluidics option, and many sample formats including atypical plates for 3D assays.

  • Analyze images of more applications

    Analyze images of more applications

    A selection of feature-rich turnkey application modules is available with MetaXpress® software. For the development of novel assays, we offer an optional custom analysis module.

  • Tailor the system to meet your needs

    Tailor the system to meet your needs

    The imager is a highly flexible solution, you can add or change many of the components as your research priorities change. Examples include enhance light engines and automation.

ImageXpress Micro 4 Imaging System


  • Multiple imaging modes

    The system offers fluorescence, confocal, widefield, colorimetric, phase contrast, and brightfield label-free imaging. 

  • Field upgradeable to confocal

    The system can be upgraded to include the proprietary AgileOptix™ technology for accurate 3D volumetric analyses. 

  • Wide dynamic range

    Low and high intensity signal quantification in a single image with >3 log dynamic range intensity detection.

  • High speed image acquisition

    Using 4X magnification with a 1536-well plate, 225K wells per day can be acquired. Greater than a million wells per week can be imaged.

  • Optional environmental control

    Temperature, humidity, and CO2 control for multi-day live-cell imaging or fast kinetic studies are available.

  • Large magnification range

    Air and oil immersion objectives ranging from 1X to 100X are available for installation into the 4-position objective changer.

Applications of ImageXpress Micro 4 High-Content Imaging System

  • Cardio


    Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.

    Read Application Note 

  • Neurite

    Neurite Outgrowth / Neurite Tracing

    Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.

    Read Application Note 

  • Stem Cell

    Stem Cell Research

    Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress Nano system paired with CellReporterXpress software for analysis has utility in all parts of the stem cells researcher’s workflow from tracking differentiation to quality control to measuring functionality of specific cell types.

    Read Application Note 

  • Toxicity Screening

    Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.

    Read Application Note 

Specifications & Options of ImageXpress Micro 4 High-Content Imaging System

*Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

Resources of ImageXpress Micro 4 High-Content Imaging System


3D Imaging of Cancer Spheroids

Whole Organism Imaging

High-Content Screening for Identifying miRNAs

Identification of Selective Inhibitors of the STAT3 Signaling Pathway

Oliver Kepp and Jayne Hesley - Hallmarks of Cancer - Detect and Quantify Cell Death Signatures with High Content Imaging

ImageXpress Micro 4 Imaging System

Multidimensional High-Throughput Imaging with the New ImageXpress Micro Confocal System

Pushing the Boundaries of High Content Screening

Preparing Assays for Genome-wide RNAi Screens Using High Content Microscopy

Multiplexed High Content Hepatoxicity Assays Using iPSC-Derived Hepatocytes

High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing in Virto Tissue Models

Simple and Flexible High Content Imaging Enabling Quantitation of Complex Biological Events

Contemporary Automation and High Content Imaging Tools for Screening Stem Cell-Derived Cardiomyocytes

Implement High-Throughput 3D Image Analysis for Samples from Subcellular Structures to Spheroids

Live Cell Imaging to Investigate the Regulation of Cell Division Timing

Using High-throughput RNA Screening to Identify Host Factors That Impact Viral Infection


Application of HCA Tools for Antibody Drug Discovery

Setting up 3D spheroid assays using high-content imaging

Setting up 3D Spheroid Assays Using High-Content Imaging

Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery

Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery

Related Products & Services of ImageXpress Micro 4 High-Content Imaging System

Featured Applications

Customer Breakthrough



MIMETAS uses the ImageXpress Confocal system to develop tissues models for their OrganoPlates®

A unique 3D organ-on-a-chip platform

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Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.