Neurite Outgrowth

  • Gain valuable insight for interpreting neurotoxicity
  • Increase efficiency with real-time acquisition and analysis
  • Simplify characterization of neurons with preconfigured Neurite Tracing analysis protocol
  • Assess data quickly using integrated visualization tools

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Simplify characterization of neurons using automated cell imaging and preconfigured neurite outgrowth analysis protocol

Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite outgrowth and is regulated by complex intracellular signaling events. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight for interpreting neurotoxic compound screening data and for elucidating factors influencing neural regeneration. Inhibition or stimulation of neurite outgrowth is implicated in a broad range of CNS disorders or injuries including stroke, Parkinson’s disease, Alzheimer’s disease and spinal cord injuries.

Acquire high quality images with more efficiency

Neurite outgrowth is assessed by the segmentation and quantification of neuronal processes. These neuronal processes can be imaged using a fluorescence microscope and quantified with manual tracing and counting when throughput is low. However, for samples in a higher-throughput microplate format, an automated imaging system paired with analysis software is a more efficient solution. Molecular Devices offers different options for automated imagers so that labs can select a system that best fits their research. Read on to see how CellReporterXpress software can be used to more efficiently acquire and analyze neuronal cell data.
  • Pre-select wells of interest during acquisition set-up

    Two wells of interest (a control vs. treated well) were selected to view at the same time during set-up of acquisition. Images were acquired using the ImageXpress Pico Automated Cell Imaging System. 

    Quickly view trends across the plate

    During acquisition, CellReporterXpress software allows users to quickly view trends across the plate using the thumbnail view. 

  • Visualize analysis of two wells of interest

    Visualize the difference between the number and length of outgrowths with CellReporterXpress software using a mask overlay to compare between stimulated and unstimulated cells.

    Analyze results for measurements of interest

    View data using a heatmap with selected measurement values including cell count, total outgrowth, average processes, and average branches.

  • Sort values to view your data of interest

    The table analysis format can be used to analyze results for measurements of interest without having to look within individual wells, as you may need to in a heatmap. The table analysis format also allows users to apply the heatmap independently to each column of values.

    Visualize trends for measurements of interest

    The scatter plot analysis format allows users to identify trends for measurements of interest against specific wells or other measurements. In this example, we see that the valproic acid treatment induces differentiation of neuroblastoma cells and that the control Neuro2a cells have fewer processes than D7 deficient Neuro2a cells in both treatments.

Resources of Neurite Outgrowth

Videos and Demos

Simplified Imaging for Quantifying Biological Complexity of iPSC-Derived Cardiomyocytes and Neurons

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