ImageXpress Confocal HT.ai High-Content Imaging System
A scalable, high-throughput, high-content screening solution with 7-channel high-intensity laser light source and machine learning capabilities.
Capture large 3D organoid and spheroid images with up to double the speed
The ImageXpress® Confocal HT.ai High-Content Imaging System utilizes a seven-channel laser light source with eight imaging channels to enable highly multiplexed assays while maintaining high throughput by using shortened exposure times. Water immersion objectives improve image resolution and minimize aberrations so scientists can see deeper into thick samples.
The powerful combination of MetaXpress® software and IN Carta™ software simplifies workflows for advanced phenotypic classification and 3D image analysis with machine learning capabilities and an intuitive user interface.
Features
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High-intensity laser light source
Quantify low and high intensity signals in a single image with >3 log dynamic range intensity detection.
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Accurate 3D measurements
MetaXpress 3D analysis module is optimized for confocal imaging, enabling 3D measurements of volume and distance.
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Wide field of view
Wide field of view enables whole-well confocal imaging and eliminates missed targets. Next-generation dual microlens enhanced spinning disk technology provides a large, flat field of view for more accurate and reproducible analysis.
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Exclusive AgileOptix™ spinning disk technology
Provides increased sensitivity with specially designed optics, high-powered laser illumination, and sCMOS sensor. Swappable disk geometries provide flexibility between speed and resolution.
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IN Carta Image Analysis Software
Leverages machine learning to improve the accuracy and robustness of high-content image analysis, delivering data insights that other technologies miss. Reduces the complexity of image analysis with intuitive guided workflows in a modern user interface.
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Multiple imaging modes
The system offers phase contrast and brightfield label-free imaging, fluorescence, widefield, and confocal imaging with water immersion optics as a standard option.
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Automated water immersion objective technology
Offers greater image resolution and sensitivity with up to 4x increase in signal leading to lower exposure times.
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Wide dynamic range
Quantifies low and high intensity signals in a single image with >3 log dynamic range intensity detection.
Images taken with same exposure times show different average intensities
IN Carta image analysis software
Powerful analytics combined with an intuitive user interface simplify workflows for advanced phenotypic classification and/or 3D image analysis. Advanced features provide the functionality to analyze data at scale and deliver real-time insights without the need for complex pre- or post-processing operations. Machine learning performs complex classification automatically with the optional Phenoglyphs module.
Cellular Image Gallery
†Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.
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Brochure
ImageXpress Confocal HT.ai
ImageXpress Confocal HT.ai
The ImageXpress® Confocal HT.ai High-Content Imaging System utilizes a seven-channel laser light source with eight imaging channels to enable highly multiplexed assays while maintaining high…
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eBook
Cellular Imaging Insights
Cellular Imaging Insights
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
Applications of ImageXpress Confocal HT.ai system
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3D Cell Imaging and Analysis
Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. Now you can generate more predictive data by incorporating technologies like the ImageXpress system with the integrated 3D Analysis Module in MetaXpress® software. This single interface will enable you to meet 3D acquisition and analysis challenges without compromise to throughput or data quality, giving you confidence in your discoveries.
3D Cell Imaging and Analysis
Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. Now you can generate more predictive data by incorporating technologies like the ImageXpress system with the integrated 3D Analysis Module in MetaXpress® software. This single interface will enable you to meet 3D acquisition and analysis challenges without compromise to throughput or data quality, giving you confidence in your discoveries.
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ.
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Cell Painting
Cell Painting is a high-content, multiplexed image-based assay used for cytological profiling. In a Cell Painting assay, up to six fluorescent dyes are used to label different components of the cell including the nucleus, the endoplasmic reticulum, mitochondria, cytoskeleton, the Golgi apparatus, and RNA. The goal is to “paint” as much of the cell as possible to capture a representative image of the whole cell.
Cell Painting
Cell Painting is a high-content, multiplexed image-based assay used for cytological profiling. In a Cell Painting assay, up to six fluorescent dyes are used to label different components of the cell including the nucleus, the endoplasmic reticulum, mitochondria, cytoskeleton, the Golgi apparatus, and RNA. The goal is to “paint” as much of the cell as possible to capture a representative image of the whole cell.
Cells in Extracellular Matrices
One common way of culturing cells in three dimensional space is through the use of extracellular matrix-based hydrogels, such as Matrigel. Cells are grown in an extracellular matrix (ECM) to mimic an in vivo environment. Differences between Matrigel and 2D cell cultures can be readily seen by their different cell morphologies, cell polarity, and/or gene expression. Hydrogels can also enable studies on cell migration and 3D structure formation, such as endothelial cell tube formation in angiogenesis studies.
Cells in Extracellular Matrices
One common way of culturing cells in three dimensional space is through the use of extracellular matrix-based hydrogels, such as Matrigel. Cells are grown in an extracellular matrix (ECM) to mimic an in vivo environment. Differences between Matrigel and 2D cell cultures can be readily seen by their different cell morphologies, cell polarity, and/or gene expression. Hydrogels can also enable studies on cell migration and 3D structure formation, such as endothelial cell tube formation in angiogenesis studies.
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COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line development, binding affinity, viral neutralization, viral titer and more with a focused effort on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including vaccines, therapeutics and diagnostics.
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
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Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.
Organoid technology / Organ-on-a-Chip
Organoid technology, like organ-on-a-chip emulates organ physiology though co-culture of cells in a supportive 3D matrix and use of microfluidic channels to perfuse nutrients or compounds over the resulting cellular structures. It is rapidly gaining popularity as a biologically relevant screening model for new drugs and toxicity.
- 3D image analysis and characterization of angiogenesis in organ-on-a-chip model
- Video: Physiologically-Relevant Tissue Models Using a High-Throughput Organ-on-a-Chip Platform
- High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
- Webinar: Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging
- Poster: Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays
Organoid technology / Organ-on-a-Chip
Organoid technology, like organ-on-a-chip emulates organ physiology though co-culture of cells in a supportive 3D matrix and use of microfluidic channels to perfuse nutrients or compounds over the resulting cellular structures. It is rapidly gaining popularity as a biologically relevant screening model for new drugs and toxicity.
- 3D image analysis and characterization of angiogenesis in organ-on-a-chip model
- Video: Physiologically-Relevant Tissue Models Using a High-Throughput Organ-on-a-Chip Platform
- High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
- Webinar: Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging
- Poster: Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays
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Organoids
Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures.
Organoids
Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures.
Spheroids
Spheroids are multi-cellular 3D structures that mimic in vivo cell responses and interactions. They can be highly reproducible and to be scaled for high-content screening. Compared with adherent cells grown in 2D monolayers, 3D growth conditions are believed to more closely reflect the natural environment of cancer cells. Acquiring measurements from these larger structures involve acquiring images from different depths (z-planes) within the body of the spheroid and analyzing them in 3D, or collapsing the images into a single 2D stack before analysis.
- 3D Imaging of Cancer Cell Spheroids
- High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
- Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures
- High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
- Acquire and analyze images of FUCCI spheroids
- Assay apoptosis in magnetically bioprinted HepG2 spheroids
Spheroids
Spheroids are multi-cellular 3D structures that mimic in vivo cell responses and interactions. They can be highly reproducible and to be scaled for high-content screening. Compared with adherent cells grown in 2D monolayers, 3D growth conditions are believed to more closely reflect the natural environment of cancer cells. Acquiring measurements from these larger structures involve acquiring images from different depths (z-planes) within the body of the spheroid and analyzing them in 3D, or collapsing the images into a single 2D stack before analysis.
- 3D Imaging of Cancer Cell Spheroids
- High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
- Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures
- High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
- Acquire and analyze images of FUCCI spheroids
- Assay apoptosis in magnetically bioprinted HepG2 spheroids
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Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress system has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types.
Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress system has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types.
Toxicity Screening
Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.
Toxicity Screening
Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.
Resources of ImageXpress Confocal HT.ai system
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Brochure
ImageXpress Confocal HT.ai
ImageXpress Confocal HT.ai
The ImageXpress® Confocal HT.ai High-Content Imaging System utilizes a seven-channel laser light source with eight imaging channels to enable highly multiplexed assays while maintain…
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eBook
Cellular Imaging Insights
Cellular Imaging Insights
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
Flyer
High-performance Customizations
High-performance Customizations
Capture more data at greater depths for 3D and thick tissue samples Scalable, high-performance, high-content screening solutions with the ImageXpress® Micro Confocal system
Scientific Poster
Neurotoxicity profiling using 3D human iPSC-derived neural spheroids
Neurotoxicity profiling using 3D human iPSC-derived neural spheroids
Test compound effects and screen for neurotoxic chemicals using 3D neural spheroids.
Infographic
3D Imaging Advantage
3D Imaging Advantage
Learn about the advantage and the technology behind 3D cellular image acquisition and analysis.
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Brochure
MetaXpress High-Content Image Acquisition and Analysis Software
MetaXpress High-Content Image Acquisition and Analysis Software
Capture high-resolution images, recognize and segment objects, calculate parameters, and then translate your information into meaningful data. At Molecular Devices, we’re inspired by…
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Brochure
3D Image Analysis Module
3D Image Analysis Module
Complex assays and three-dimensional cell models better represent tissue biology and cell interactions, making them more relevant for many toxicity and drug screening assays. The abi…
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Scientific Poster
Automated 3D cell-based assays using a novel flow chip system and high-content imaging
Automated 3D cell-based assays using a novel flow chip system and high-content imaging
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translation research. Significant progress has b…
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Scientific Poster
Using a laser light source for high-content automated imaging
Using a laser light source for high-content automated imaging
In this study, we demonstrate improvement in assay sensitivity, precision, and speed of acquisition with a new configuration of the ImageXpress® Confocal HT.ai High-Content Imaging S…
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Scientific Poster
Simplified workflow for phenotypic profiling based on the Cell Painting assay
Simplified workflow for phenotypic profiling based on the Cell Painting assay
Multiparametric high-content screening approaches, such as the Cell Painting assay, are increasingly being used in many applications ranging from drug discovery programs to functiona…
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Scientific Poster
Lung organoids as an assay model for in vitro assessment of toxicity effects by 3D high-content imaging and analysis
Lung organoids as an assay model for in vitro assessment of toxicity effects by 3D high-content imaging and analysis
Organoid models have increasingly gained popularity in biologic research and screening to recapitulate complexity of real tissues. To model the in vivo human lungs, we have cultured…
Application Note
Improve sensitivity, speed, and assay quality for complex biological assays
Improve sensitivity, speed, and assay quality for complex biological assays
Here, we demonstrate improvement in assay sensitivity, quality, and speed of acquisition with the ImageXpress® Confocal HT.ai, our high-content imaging laser-based system.
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Application Note
High-content phenotypic profiling using the Cell Painting assay
High-content phenotypic profiling using the Cell Painting assay
Here, we present a complete workflow for a cell painting assay that can be easily implemented using the ImageXpress Micro system and image analysis with machine learning capabilities
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Application Note
3D cell-based assays using a novel fluidics flowchip technology and high-content imaging
3D cell-based assays using a novel fluidics flowchip technology and high-content imaging
Known as lab-on-a-chip systems, microfluidics gained popularity for its demonstrated success manipulating small volumes of fluid to control chemical, biological, and physical processes.
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Scientific Poster
3D imaging and analysis of angiogenesis in the organ-on-a-chip platform
3D imaging and analysis of angiogenesis in the organ-on-a-chip platform
Angiogenesis is the physiological process of formation and remodeling of new blood vessels and capillaries from pre-existing blood vessels.1 This can be achieved through e…
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Application Note
High-throughput screening of 3D cell cultures with multiple high density scaffold-free spheroids for cancer toxicity studies
High-throughput screening of 3D cell cultures with multiple high density scaffold-free spheroids for cancer toxicity studies
3D spheroid models for cancer research are gaining popularity because they better mimic the in vivo tissue architecture, gene expression and metabolic profile of tumors compared to…
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eBook
High-content imaging for diverse 3D cell culture models
High-content imaging for diverse 3D cell culture models
Investigate diverse 3D models and resolve common challenges experienced in 3D cell culture assays.
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Scientific Poster
AI-based analysis of complex biological phenotypes
AI-based analysis of complex biological phenotypes
Cell-based phenotypic assays have become an increasingly attractive alternative to traditional in vitro and in vivo testing in pharmaceutical drug development and toxicological safet…
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Application Note
3D image analysis and characterization of angiogenesis in organ-on-a-chip model
3D image analysis and characterization of angiogenesis in organ-on-a-chip model
Angiogenesis is the physiological process of formation and remodeling of new blood vessels and capillaries from pre-existing blood vessels. This can be achieved through endothelial…
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Application Note
Phenotypic characterization of neuroactive compound effects
Phenotypic characterization of neuroactive compound effects
The FLIPR Penta system is powered by a high-speed EMCCD camera and the new ScreenWorks Peak Pro 2 software. The system allows measurement and analysis of complex patterns of calcium…
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Scientific Poster
High-throughput assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocyte
High-throughput assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocyte
Development of biologically relevant and predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery. The focus of this study w…
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Scientific Poster
Automated Long-term 3D Cell-Based Toxicity Studies Using a Flowchip System
Automated Long-term 3D Cell-Based Toxicity Studies Using a Flowchip System
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translation research.1 Significant progress has…
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Scientific Poster
Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
Cell-based phenotypic assays have become an increasingly attractive alternative to traditional in vitro and in vivo testing in pharmaceutical drug development and toxicological safet…
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Application Note
Monitor mitochondria dynamics and phenotype with high-content imaging
Monitor mitochondria dynamics and phenotype with high-content imaging
Mitochondria are the main energy source for cells and play a key role in regulating cellular metabolism. Mitochondria can change their structures depending on environmental conditions…
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Scientific Poster
Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
To speed up the development of more effective and safer drugs, there is an increasing need for more complex, biologically relevant and predictive cell-based assays for drug discovery…
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Scientific Poster
Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays
Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays
The purpose of these studies was to determine if water immersion objectives, used to improve image quality in complex biological assays, could be used in a high-throughput environmen…
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Flyer
Water Immersion Objectives for the ImageXpress Micro Confocal system
Water Immersion Objectives for the ImageXpress Micro Confocal system
Gain the sensitivity to capture more phenotypic data at greater depths. Our high-content imagers and expert team are here to help your lab simplify imaging and analysis of complex, 3…
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Application Note
A high-content tube formation assay using an 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 angiogenesis model
A high-content tube formation assay using an 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 angiogenesis model
Angiogenesis, the formation of new blood vessels from existing ones, is a critical step involved in various biological processes, such as endothelial sprouting, proliferation,…
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Application Note
Measure cell migration using a simple scratch assay with timelapse live-cell imaging
Measure cell migration using a simple scratch assay with timelapse live-cell imaging
The movement or migration of cells has long been studied to elucidate the physiological mechanisms of angiogenesis, embryogenesis, cancer metastasis, immune responses, and wound…
Presentations
Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures
Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures
Expertise in human iPSC-derived neural and cardiac platforms; Focus on adapting platforms to HTS and producing assay-ready solutions to accelerate drug discovery; Neural platform:…
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Application Note
Explore cardiac function by measuring calcium oscillation or contraction patterns
Explore cardiac function by measuring calcium oscillation or contraction patterns
Understanding the impact of drugs on cardiac system early in the development process is critical to improve the safety of drugs getting to market. Cardiotoxicity is believed to be one…
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Presentations
Advantages and challenges of phenotypic screening in 3D culture including ECM
Advantages and challenges of phenotypic screening in 3D culture including ECM
Learn more about the advantages and challenges of using 3D culture in phenotypic screening
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Scientific Poster
High-throughput single-cell contractility measurements using FLECS with ImageXpress Micro
High-throughput single-cell contractility measurements using FLECS with ImageXpress Micro
Track contractility of each single cell within each population over long experimental times.
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Application Note
High-throughput screening of single-cell contractile force using FLECS Technology
High-throughput screening of single-cell contractile force using FLECS Technology
The ability of mammalian cells to generate mechanical forces—to push, pull, or squeeze—is an intrinsic capability that is used by cells both individually and together as tissue to…
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Scientific Poster
A novel high-throughput solution for screening drugs with a light-crosslinked VersaGel 3D tumor culture model and automated high-content imaging
A novel high-throughput solution for screening drugs with a light-crosslinked VersaGel 3D tumor culture model and automated high-content imaging
Screen models that mimic in vivo tumor microenvironment using novel 3D cell culture platform, VersaGel and Symphony
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Scientific Poster
Measure cell migration using automated cell imaging and analysis
Measure cell migration using automated cell imaging and analysis
Effectively image and analyze cell migration using a scratch assay or a dissolvable biocompatible gel
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Application Note
Acquire data 10X faster from 3D spheroids cultured using Symphony® and VersaGel®
Acquire data 10X faster from 3D spheroids cultured using Symphony® and VersaGel®
Most in vitro cell culture work is performed in two-dimensions (2D), where cells grow on a flat surface. However, culturing cells in 2D to screen drugs provides a limited picture of…
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eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
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eBook
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
This new eBook discusses early drug safety testing using HTS iPSC-derived cells with imaging. Download it now!
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Application Note
Counting cells with the Transmitted Light Analysis Module in MetaXpress
Counting cells with the Transmitted Light Analysis Module in MetaXpress
Label-free cellular assays are required for a multitude of biological applications that monitor the cell number, proliferation, health, confluency, and cytotoxicity. In this…
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Application Note
Analyze experimental results using curve fitting with MetaXpress 6.5 software
Analyze experimental results using curve fitting with MetaXpress 6.5 software
The ability to annotate your plate, plot your data into a graph, and produce a variety of curves and readouts including IC50 values and benchmark dose or concentration for your…
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eBook
Acquire and analyze 3D images like a pro
Acquire and analyze 3D images like a pro
There has been significant progress in the development of 3D models and techniques during the last few years. Methods include biodegradable scaffolds, organ-on-a chip structures, or…
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Scientific Poster
High-Throughput Assays for Characterizing Viability and Morphology of 3D Cancer Spheroid Cultures
High-Throughput Assays for Characterizing Viability and Morphology of 3D Cancer Spheroid Cultures
Three-dimensional (3D) in vitro models span the gap between two-dimensional cell cultures and whole-animal systems. By mimicking features of the in vivo environment and taking advant…
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Brochure
Imaging Solutions to Answer Your Biological Questions
Imaging Solutions to Answer Your Biological Questions
Learn about our imaging family and which solution is best suited to answer your biological questions.
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Scientific Poster
Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Development of predictive in vitro assays for early toxicity evaluation is extremely important for improving the drug development process and reducing drug attrition rates during cli…
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Scientific Poster
Novel High Content Stem Cell-based Assays for Screening
Novel High Content Stem Cell-based Assays for Screening
The biopharma industry is continuing to adapt more cell-based assays for primary and secondary screening because of higher biological relevance and increased value of information.…
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Application Note
Measuring mitochondrial shape and distribution using the ImageXpress Micro High-Content Imaging System
Measuring mitochondrial shape and distribution using the ImageXpress Micro High-Content Imaging System
Using high-content imaging assays allow cells cultured and treated in microplates to be imaged alive without wash steps for real-time studies of cells in their most natural state,…
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Application Note
Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
Cell models are becoming more complex in order to better mimic in vivo microenvironments and provide greater predictivity for compound efficacy and toxicity. There is an increasing…
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Application Note
High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
Establishment of physiologically-relevant in vitro models is crucial to further understanding of the mechanisms of neurological diseases as well as targeted drug development. While…
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Application Note
Automating the generation and analysis of 3D cell cultures in Matrigel®
Automating the generation and analysis of 3D cell cultures in Matrigel®
Researchers are turning to three-dimensional (3D) cell culture as a way to increase the biological relevance of their disease models and predictive value of drug studies. These 3D…
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Application Note
Automation of 3D spheroid cultures in ultra-low attachment plates
Automation of 3D spheroid cultures in ultra-low attachment plates
We have previously described the automated plating, treatment, and analysis of 3D spheroids formed in hanging drop
plates (see Automated 3D Cell Culture and Screening by Imaging… -
Application Note
Multi-parameter imaging assay for measuring toxicity in a tumor model
Multi-parameter imaging assay for measuring toxicity in a tumor model
There is an increasing interest in using three-dimensional (3D) spheroids for modeling cancer and tissue biology to accelerate translational research. The goal of this study was to…
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Application Note
3D analysis and morphometric characterization of compound effects on cancer spheroid cultures
3D analysis and morphometric characterization of compound effects on cancer spheroid cultures
Cellular transformation/tumorigenicity assays using cultures of cells in semi-solid media (soft agar or Matrigel) has been well established for cancer research1,2,5. The assay requires…
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Application Note
High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
There is increasing interest in exploring the use of three-dimensional (3D) spheroids for modeling developmental and tissue biology with the goal of accelerating translational research…
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Application Note
High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics
In recent years, there has been significant progress in development of in vitro aggregates of tumor cells for use as models for in vivo tissue environments. When seeded into a well of…
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Application Note
3D Imaging of Cancer Cell Spheroids
3D Imaging of Cancer Cell Spheroids
Many cancer cell lines will form spheroids if cultured on a favorable three dimensional (3D) matrix. These spheroids are believed to represent tumor physiology more closely than cells…
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Application Note
Live cell kinetics assay utilizing the ImageXpress Micro System
Live cell kinetics assay utilizing the ImageXpress Micro System
The ImageXpress® Micro Confocal/ XLS Widefield High-Content Imaging System from Molecular Devices includes optional Fluidics and Environmental Control modules, enabling single-channel…
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Application Note
Assaying cardiotoxicity with ImageXpress high-content screening systems
Assaying cardiotoxicity with ImageXpress high-content screening systems
The discovery and development of novel drugs is a difficult and expensive process due to lack of tools and techniques to accurately reproduce the human physiological response in the…
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Application Note
Visualize subcellular vesicles to quantitate autophagy in neuronal cells
Visualize subcellular vesicles to quantitate autophagy in neuronal cells
Autophagy is an intracellular catabolic process that sequesters and degrades proteins and organelles that have either been recognized as faulty or are simply no longer needed by the…
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Application Note
Increase sensitivity in no-wash assays using confocal imaging
Increase sensitivity in no-wash assays using confocal imaging
Screening of hybridoma supernatants for antibodies directed against cell-surface antigens is an important step in the discovery and development of antibodies for vaccines or…
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Application Note
High-throughput imaging assays using zebrafish, a model organism for human disease
High-throughput imaging assays using zebrafish, a model organism for human disease
Recently, zebrafish-based screening has gained favor as an alternative to mammalian screening due to cost, throughput and reduced ethical concerns. Zebrafish are a useful model for…
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Application Note
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis is an important mechanism signaling programmed death of cells in normal processes such as embryonic development, as well as in diseases including cancer and neurodegenerative…
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Application Note
High-content screening of neuronal toxicity using iPSC-derived human neurons
High-content screening of neuronal toxicity using iPSC-derived human neurons
The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. Neurotoxicity can cause temporary or…
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Application Note
Intelligent time-lapse imaging to enhance live cell assay development
Intelligent time-lapse imaging to enhance live cell assay development
The ability to monitor responses in living cells over a specific period of time offers cell biologists several key advantages for assay development. For routine cellbased screening,…
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Application Note
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
Drug-induced hepatotoxicity is an important cause for liver injury and acute liver failure. Thus highly predictive assays for safety and efficacy testing are crucial for improving drug…
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Application Note
Monitoring BNP Expression and Cell Size to Determine Hypertrophic Response in Human Cardiomyocytes
Monitoring BNP Expression and Cell Size to Determine Hypertrophic Response in Human Cardiomyocytes
Cardiac hypertrophy is a condition associated with many heart diseases such as myocardial infarction, ischemia, hypertension, valvular dysfunctions, and is also observed as a toxic…
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Flyer
ImageXpress Micro Confocal High-Content Imaging System
ImageXpress Micro Confocal High-Content Imaging System
The ImageXpress® Micro Confocal High-Content Imaging System provides improved quantification for live or fixed cell assays. This versatile imaging system features a unique confocal t…
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Scientific Poster
High-Content Assay for Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform
High-Content Assay for Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform
The absence of physiologically relevant in vitro models of the nervous system is an important limitation in using 3D cultures for assay development applicable for neurodegenerative d…
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Scientific Poster
Phenotypic Characterization of Compound Effects on iPSC-derived Cardiac & Liver Spheroids
Phenotypic Characterization of Compound Effects on iPSC-derived Cardiac & Liver Spheroids
Explore PDF & learn the use of fast kinetic fluorescence and 3D image analysis for Phenotypic characterization of compound effects on iPSC-derived Cardiac and Liver Spheroids.…
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Scientific Poster
In Vitro Assessment of Drug Effects on Human iPSC-Derived Cardiac Spheroid Cultures
In Vitro Assessment of Drug Effects on Human iPSC-Derived Cardiac Spheroid Cultures
for modeling developmental and tissue biology with the goal of accelerating translational research. Such 3D models can provide different perspectives from traditional 2D cultures on…
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Scientific Poster
Increase Sensitivity with Confocal Acquisition in a High-Throughput Imaging Assay for Detecting DNA Damage and Genotoxicity
Increase Sensitivity with Confocal Acquisition in a High-Throughput Imaging Assay for Detecting DNA Damage and Genotoxicity
Learn how to improve sensitivity of DNA damage detection & genotoxicity sensitivity with confocal acquisition to detect nuclear punctae in a high throughput imaging assay.
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Scientific Poster
Screening Cell Cycle Inhibitors using Automated High-Content Imaging
Screening Cell Cycle Inhibitors using Automated High-Content Imaging
Monitoring treatment effects on the cell cycle is particularly relevant to progressing oncology research and drug discovery. For instance, compounds known to inhibit mitosis are ofte…
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Scientific Poster
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is one of the main challenges in drug discovery. There is an increasing in…
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Scientific Poster
Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
Read more about cell based assays for measuring the impact of pharmacological compounds on the rate of beating cardiomyocytes with different assay platforms.
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Scientific Poster
Morphometric Characterization of Cancer Spheroid Cultures Using Confocal Imaging and 3D Image Analysis
Morphometric Characterization of Cancer Spheroid Cultures Using Confocal Imaging and 3D Image Analysis
There is an emerging interest in using three-dimensional (3D) organoid cultures for modeling tissue biology and cancer. Development of higher throughput assays to quantify phenotypic…
Imaging and Analysis of a 3D Airway Organoid Model
ImageXpress Confocal HT.ai / IN Carta Image Analysis Software Introduction
Immunology and Vaccine Development Workflow
Hybridoma Workflow
Disease modeling in the 21st century: Automated organoid assays with 3D imaging
High-throughput, organoid-derived organ-on-a-chip systems for drug discovery and disease modelling
Unleash the power of Cell Painting
3D cell culture, tissue clearing, & high-content imaging in the quest of effective solutions to NAFLD
Transitioning high-content assays to 3D: Scientific opportunities and imaging challenges
Gain deeper insights into cellular 3D structures with water immersion objectives
An AI-based approach to high-content phenotypic characterization of human iPSC-derived neuronal cells
Implementing 3D Neural Spheroids in Drug Discovery
Accelerate your screening with high-content and automated imaging
Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening
ImageXpress Micro Confocal Virtual Tour
Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow
LabTube Meets Molecular Devices & MIMETAS, Susan Murphy & Sebastiaan Trietsch
Plate annotation and curve fitting in MetaXpress
Quickstart Guide: Plate acquisition on the ImageXpress Micro Confocal using MetaXpress
Quickstart Guide: Review images on the ImageXpress Micro Confocal using MetaXpress
Basic workflow from image acquisition to analysis on the ImageXpress Micro Confocal using MetaXpress
Developing high-throughput organ-on-a-chip tissue models for drug discovery using high-content imaging
Toxicity studies of iPSC-derived cardiomyocytes and neuronal spheroids
Optimizing high-content screening tools for physiologically-relevant 3D in vitro models
Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform
3D Imaging of Cancer Spheroids
High-Content Screening for Identifying miRNAs
Identification of Selective Inhibitors of the STAT3 Signaling Pathway
Oliver Kepp and Jayne Hesley - Hallmarks of Cancer - Detect and Quantify Cell Death Signatures with High Content Imaging
Multidimensional High-Throughput Imaging with the New ImageXpress Micro Confocal System
Pushing the Boundaries of High Content Screening
Preparing Assays for Genome-wide RNAi Screens Using High Content Microscopy
Multiplexed High Content Hepatoxicity Assays Using iPSC-Derived Hepatocytes
High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 Tissue Models
Implement High-Throughput 3D Image Analysis for Samples from Subcellular Structures to Spheroids
Live Cell Imaging to Investigate the Regulation of Cell Division Timing
Using High-throughput RNA Screening to Identify Host Factors That Impact Viral Infection
Application of HCA Tools for Antibody Drug Discovery
Setting up 3D Spheroid Assays Using High-Content Imaging
Physiologically-Relevant Tissue Models Using a High-Throughput Organ-on-a-Chip Platform
Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery
StemoniX microBrain 3D Assay Ready Plates for HTS
Related Products & Services of ImageXpress Confocal HT.ai system
Expand your research using flexible, high-performance imaging solutions
Molecular Devices provides flexible options for the ImageXpress Confocal HT.ai system to meet your research needs and to easily capture images from different sample formats, including hanging drops and in round or flat bottom plates, for monitoring cell health kinetics under environmental control, and more. With over 30+ years of imaging expertise, we can help you select the right options to ensure the best images for your assay.
Standard hardware options
Water immersion objectives
20X, 40X, and 60X water immersion objectives improve the geometric accuracy during acquisition and reduce light refraction for brighter intensity at lower exposure times.
Transmitted light tower
Our transmitted light tower enables acquisition of high contrast images for unstained cells which can be easily viewed or separated from background.
Environmental Control
Environmental control maintains temperature and humidity levels while minimizing evaporation for multi-day, live cell, time-lapse imaging.
Customization options
Molecular Devices can successfully tailor the ImageXpress Confocal HT.ai system to include customized software and hardware including the features described below, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support.
Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms
Deep tissue penetrating confocal disk module
The deep tissue penetrating, confocal disk module reduces crosstalk to improve out-of-focus light suppression and penetrate deeper into tissue.
Turnkey, high-throughput long term kinetics
Schedule and image multiple plates over long periods of time while keeping consistent temperature, O2 (Hypoxia), CO2, and humidity conditions. Expand live cell walk-away capacity to 200+ plates.
Scale up robotic automation
Increase throughput, eliminate human errors, maintain sterility, and achieve consistent sample handling. Modular automation design—components can be added in modules and are upgradeable.
Deep Tissue Penetrating, Confocal Disk Module
Specialized deep tissue penetrating, confocal disk module, combined with a laser light source, improves light penetrance for deeper tissue penetration, resulting in sharper images with improved resolution when imaging thick tissue samples†.
- Improve suppression of out-of-focus light
- Reduce haze (pinhole crosstalk)
- Penetrate deeper into thick tissue samples for sharper images
Images taken at the same exposure.
†Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance
Featured Applications
3D Cell Imaging and Analysis
Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue…
Cancer Research
Cancer researchers need tools that enable them to more easily study the complex and often poorly…
Cell Painting
Cell Painting is a high-content, multiplexed image-based assay used for cytological profiling. In a…
Cells in Extracellular Matrices
One common way of culturing cells in three dimensional space is through the use of extracellular…
COVID-19 and Infectious Disease Research
Here we've addressed common applications in infectious disease research including cell line…
Live Cell Imaging
Live cell imaging is the study of cellular structure and function in living cells via microscopy.…
Neurite Outgrowth / Neurite Tracing
Neurons create connections via extensions of their cellular body called processes. This biological…
Organoid technology / Organ-on-a-Chip
Organoid technology, like organ-on-a-chip emulates organ physiology though co-culture of cells in a…
Organoids
Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic…
Spheroids
Spheroids are multi-cellular 3D structures that mimic in vivo cell responses and interactions. They…
Stem Cell Research
Pluripotent stem cells can be used for studies in developmental biology or differentiated as a…
Toxicity Screening
Screening for off-target or toxic effects is very important during the development of new drugs and…
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