Cell Counting

Cell Counting

Assessing proliferation, cytotoxicity, or confluency using cell counting can be quickly accomplished with either brightfield or fluorescent imaging using ImageXpress Nano or ImageXpress Pico Automated Imaging Systems with integrated CellReporterXpress Analysis software.

Cytometry is the quantitation of cells based on particular characteristics such as size, morphology, or protein expression levels. Image cytometry is a powerful technique that combines low magnification microscopy with imaging and analysis tools. Image cytometry is useful for providing information on a broad range of cellular characteristics on a large population of cells in a single assay. Image cytometry can be used on unlabeled or fluorescently labeled cells and can be performed multiple times on a population to provide information about cellular dynamics over time.

  • Cytotoxicity

    Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a way to easily screen cytotoxicity in treated cells is critical to identifying new therapeutic treatments or understanding cellular signaling pathways that affect cell health. Common indicators of cytotoxicity include the ATP level in a cell population and integrity of cellular membranes, both of which can be measured using a variety of microplate-based assays.

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    Cell Viability

    Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.

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  • Cell Migration

    Cell migration, the movement of cells from one location to another, is a critical component of both normal and abnormal biological processes. The migration of fluorescently labeled cells from one area of a microplate well to another can be monitored using a cellular imaging system. Automated analysis software calculates the amount of cell migration in each well.

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    Cell Cycle Analysis

    Transition of a cell through the cell cycle can be monitored using fluorescently labeled cell cycle proteins that are cyclically expressed and degraded. FUCCI technology is based on the overexpression the cell cycle-dependent proteins geminin and Cdt1, respectively fused to a green fluorophore and a red fluorophore. Cdt1 levels peak in G1 phase, so cells in G1 appear green; geminin levels rise in late S, G2 and M phase, so cells in these phases appear red.

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