Established in the late 1970s from the peripheral blood of a young boy with T-cell leukemia, Jurkat cells are an immortalized T-lymphocyte cell line. They have been used to study T-cell leukemia, T-cell signaling, and cancer cell sensitivity to drug treatments. These small, round cells grow readily in suspension and have also been used as a model system for various cell viability and apoptosis assays as demonstrated here.
Thanks to their rounded morphology and uniform size, Jurkat cells are fairly easy to image and count. For StainFree cell counting, the predefined ‘CellsC’ in SoftMax Pro Software works great.
Jurkat Cells Analysis Toolkit
- SpectraMax® i3x Multi-Mode Microplate Detection Platform
- SpectraMax® MiniMax™ 300 Imaging Cytometer
- SoftMax® Pro Software
|Parameter||Setting for cell counts|
|Optical configuration||SpectraMax MiniMax 300 Imaging Cytometer|
|Wavelength settings||Transmitted light|
|Image acquisition settings||Exposure: 7 ms|
|Image analysis settings||Analysis type: Discrete Object Analysis
Wavelength for finding objects: TL
|Find objects||Settings: 'CellsC' predefined setting|
About StainFree Cell Detection Technology
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.
The SpectraMax i3 Multi-Mode Microplate Platform with MiniMax 300 Imaging Cytometer uses unique, patent-pending StainFree Cell Detection Technology that allows you to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI, which intercalates with DNA, or live cell dyes that are actually toxic to cells in the long term.