COVID-19 RESPONSE - We are committed to supporting our scientific community during this pandemic. Learn more 

Unlimited breadth of user-upgradeable application modules expands research capabilities

The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.

  • Capture flash assays with ease

    Capture flash assays with ease

    The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays.

  • Reduce background noise

    Reduce background noise

    The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light.

  • Increase fluorescence performance

    Increase fluorescence performance

    Powerful combination of Xenon flash lamp and LEDs provides unmatched signal strength and superior sensitivity with Spectral Fusion™ Illumination.

SpectraMax i3x Multi Mode Detection Platform

SpectraMax i3x Multi-Mode Detection Platform


  • User-configurable applications

    User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.

  • No-stain cell analysis

    The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.

  • Multi-channel functionality

    Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.

  • Cellular pathway analysis

    Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.

Add Cellular Imaging to your Plate Reader Workflow


Observe the viability of your cells with the field-upgradeable cellular imaging option for the SpectraMax i3x Multi-mode Detection Platform.

The field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity, cell proliferation, and protein expression. Now with StainFree™ Cell counting Technology, additional brightfield analysis features, and fluorescent green and red detection channels.

Learn more






Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational speci?cations
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs


Applications of SpectraMax i3x Multi-Mode Microplate Reader

  • Absorbance


    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 


    AlphaScreen Technology

    AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular binding. Donor and acceptor beads are attached to biomolecules, and when the beads are brought into proximity (via binding), a cascade of chemical reactions occurs, amplifying a signal that is detected on the SpectraMax® i3x and Paradigm® Multi-Mode Microplate Readers.

    Read Application Note 

  • Cell Imaging > Cell Cycle Analysis

    Cell Cycle Analysis

    Transition of a cell through the cell cycle can be monitored using fluorescently labeled cell cycle proteins that are cyclically expressed and degraded. FUCCI technology is based on the overexpression the cell cycle-dependent proteins geminin and Cdt1, respectively fused to a green fluorophore and a red fluorophore. Cdt1 levels peak in G1 phase, so cells in G1 appear green; geminin levels rise in late S, G2 and M phase, so cells in these phases appear red.

    Read Application Note 

    Cell Imaging > Cell Migration

    Cell Migration Imaging

    Cell migration, the movement of cells from one location to another, is a critical component of both normal and abnormal biological processes. The migration of fluorescently labeled cells from one area of a microplate well to another can be monitored using a cellular imaging system. Automated analysis software calculates the amount of cell migration in each well.

    Read Application Note 



    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 



    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  

  • Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

    Learn more  

    Kinase/Phosphatase Assays

    Kinase/Phosphatase Assays

    Kinases are one of the most critical targets in drug discovery today. These enzymes are key components in cellular signaling pathways, and disruption to their function causes a variety of diseases, such as metabolic diseases, certain cancers, and cardiac disease. The functionally related phosphatases and phosphodiesterases are also important screening targets.

    Read Application Note 

  • Luminescence


    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

    Learn more  


    Nanoparticles Mammalian Cells

    Nanomaterials are typically less than 100 nm in diameter, small enough to penetrate mammalian cells. They can be synthesized in many shapes, such as rods, tubes, and particles, as well as in different materials. Targeting or therapeutic molecules can be readily attached to nanoparticles, and when these are delivered systemically, the targeting molecules enable detection of certain cell populations, such as tumor cells, while attached therapeutic compounds can act on the targeted cells.

    Read Application Note 

  • Spheroids in 3D biology

    3d Minimax

    Cancer is a leading cause of death worldwide, but researchers are making enormous strides towards understanding its underlying mechanisms and working towards new treatments. Spheroids, 3D aggregates of cells in culture, have emerged as a valuable research tool that provides more physiological relevance than traditional 2D cell culture.

    Learn how spheroids formed using a variety of cancer cell types are analyzed on the SpectraMax® i3x microplate reader with MiniMax™ cytometer using methods featured in our application notes:



    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

    Learn more  

  • Western Blot

    Western Blot Detection

    Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. We’ll also introduce you to a novel, first-of-its-kind western blot detection system on a multi-mode microplate reader.

    Learn more 

Specifications & Options of SpectraMax i3x Multi-Mode Microplate Reader


*Using lowest settings and speed read when available.

Resources of SpectraMax i3x Multi-Mode Microplate Reader

Videos & Webinars
Immunology and Vaccine Development Workflow

Immunology and Vaccine Development Workflow

Hybridoma Workflow

Hybridoma Workflow

Week 5 Video Thumbnail

Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?

Week 4 Video Thumbnail

Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy

Week 3 Video Thumbnail

Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"

 Week 1 Video Thumbnail

Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!

Week 2 Video thumbnail

Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!

Microplate Readers from ELISA to AlphaScreen

Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen

SpectraMax i3x

Microplate based Detection

Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening

ELISA endpoint protocol

How to configure an ELISA endpoint protocol

Magnetic 3D

Magnetic 3D Bioprinting, 3D Cell Culture in a 2D Workflow

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Novel Ways to Monitor Cell Growth and Cytotoxicity: StainFree Technology

Novel Ways to Monitor Cell Growth and Cytotoxicity: StainFree Technology

SpectraMax i3x Multi Mode Detection Platform

SpectraMax i3x Multi-Mode Detection Platform

SpectraMax® i3x Multi-Mode Microplate Reader

SpectraMax® i3x Multi-Mode Microplate Reader System

System Options

Product Part Number
SpectraMax i3x Multi-Mode Microplate Reader with SoftMax Pro Software i3x
SpectraMax MiniMax 300 Imaging Cytometer 5024062
SoftMax Pro 7.x GxP Software by license bundle
SpectraTest (ABS2) Absorbance Validation Plate 0200-6191
SpectraTest (LM1) Luminescence Validation Plate 0200-6186
SpectraTest (FL1) Fluorescence Validation Plate 0200-5060
SpectraDrop Micro-Volume Starter Kit 0200-6262
SpectraDrop Micro-Volume HTS Kit 0200-6267

SpectraMax Detection Cartridge Options

Product Name Part Number
AlphaScreen Detection Cartridge (384 STD) 0200-7017POS
AlphaScreen Detection Cartridge (384 HTS) 0200-7018POS
AlphaScreen Detection Cartridge (1536 HTS) 0200-7019POS
HTRF Detection Cartridge 0200-7011POS
TRF Detection Cartridges 0200-7008POS
Fluorescence Polarization Detection Cartridge
Fluorescence Polarization Detection Cartridge (Rhodamine) 0200-7010POS
Luminescence Detection Cartridge 
(Glow for 96-well plates)
Luminescence Detection Cartridge 
(Glow 96- and 384-well plates)
Luminescence Detection Cartridge 
(Glow 96-, 384-, and 1536-well plates)
Dual Glow Luminescence Detection Cartridge
Fluorescence Intensity Detection Cartridge 
Fluorescence Intensity Detection Cartridge
Fluorescence Intensity Detection Cartridge
ScanLater Western Blot Detection Cartridge 0200-7027
SpectraMax Injector Cartridge with SmartInject™ Technology 0200-0729

Related Products & Services of SpectraMax i3x Multi-Mode Microplate Reader