U937 cells were isolated from a histiocytic lymphoma of a 37-year-old male patient in 1974. Because they are one of just a few cell lines available that express many monocytic characteristics, they are often used to study the behavior and differentiation of monocytes. U937 cells undergo apoptosis when treated with granulocyte-macrophage colony-stimulating factor (GM-CSF; Okuma et al., 2000), making them a useful model for studies of apoptotic cell signaling.
U937 cells grow in suspension and have a round shape that makes them easy to count. For StainFree counting, simply use the predefined analysis setting ‘CellsC’ in the dropdown menu in SoftMax Pro Software to analyze cell images with a single click.
U937 Cells Analysis Toolkit
- SpectraMax® i3 Multi-Mode Microplate Detection Platform
- SpectraMax® MiniMax™ 300 Imaging Cytometer
- SoftMax® Pro Software
|Parameter||Setting for cell counts|
|Optical configuration||SpectraMax MiniMax 300 Imaging Cytometer|
|Wavelength settings||Transmitted light (TL)
541 nm (green fluorescence)
|Image acquisition settings||TL exposure: 7 ms
TL Focus adjustment: -5 µm
|541 exposure: 1 ms
541 Focus adjustment: 40 µm
|Image analysis settings||Analysis type: Discrete Object Analysis
Wavelength for finding objects: TL (StainFree) or 541 (green nuclei)
|Find objects||TL: CellsC (predefined)
541: Set Size and Intensity: Size = 10-30 µm, Intensity Above Background = 150
About StainFree Cell Detection Technology
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.
The SpectraMax i3/i3x Multi-Mode Microplate Platform with MiniMax 300 Imaging Cytometer uses unique, patent-pending StainFree Cell Detection Technology that allows you to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI, which intercalates with DNA, or live cell dyes that are actually toxic to cells in the long term.