HepG2 is a cell line derived from the liver tissue of a patient with hepatocellular carcinoma (HCC). It is often used as a model system for HCC as well as for studies of drug metabolism and toxicity. Cultures are adherent, with epithelial morphology, and tend to grow in small aggregates that make the counting of individual cells difficult. Under the right culture conditions, they can form distinct apical and basal surfaces amenable to studies of liver disease.
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Figure 1: StainFree determination of % covered area
Figure 2: StainFree vs. fluorescent analysis of % covered area
Figure 3: EarlyTox Caspase-3/7 NucView 488 Assay
Figure 4: EarlyTox Caspase-3/7 NucView 488 Assay (time course)
Figure 5: EarlyTox Caspase-3/7 R110 Assay Kit and EarlyTox Glutathione Assay Kit
Tip: HepG2 cells grow in aggregates or clumps of irregularly shaped cells that are indistinguishable by eye. A useful way to quantify HepG2 cell growth or confluence is by using StainFree™ Technology to determine the area covered by cells. Images collected in the TL channel can be easily analyzed using the Field Analysis setting. The predefined ‘Cells’ analysis setting can be used, or a custom analysis can be designed by using the drawing tools in SoftMax Pro Software.
HepG2 Cells Analysis Toolkit
- SpectraMax® i3 Multi-Mode Microplate Detection Platform
- SpectraMax® MiniMax™ 300 Imaging Cytometer
- SoftMax® Pro Software
|Parameter||Setting for cell counts|
|Optical configuration||SpectraMax MiniMax 300 Imaging Cytometer|
|Wavelength settings||Transmitted light|
|Image acquisition settings||Exposure: 7 ms (TL), 5 ms (541)|
|Image analysis settings||Analysis type: Field Analysis
Wavelength for finding objects: TL
|Find objects||Settings: Custom setting|
About StainFree Cell Detection Technology
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.
The SpectraMax i3 Multi-Mode Microplate Platform with MiniMax 300 Imaging Cytometer uses unique, patent-pending StainFree Cell Detection Technology that allows you to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI, which intercalates with DNA, or live cell dyes that are actually toxic to cells in the long term.