Human umbilical vein endothelial cells (HUVEC) are primary cells isolated from the vein of the umbilical cord. They are a model system for studying endothelial cell function, with applications including hypoxia, inflammation, oxidative stress, response to infection, and both normal and tumor-associated angiogenesis. Endothelial cell activation, an inflammatory response, can be induced by cytokines such as TNF-α and IFN-γ and results in upregulation of cell adhesion molecules like VCAM-1/CD106, whose upregulation can be quantified using fluorescently labeled antibodies and cellular imaging.
HUVEC cells have a typical cobblestone appearance. For StainFree counting, the preconfigured setting ‘CellsB’ in the SoftMax Pro Software image analysis settings works very well. Alternatively, when counting cells whose morphology has changed due to treatment with cytokines, etc., creating a new analysis setting by drawing on the cell images may be helpful.
HUVEC Cells Analysis Toolkit
- SpectraMax® i3 Multi-Mode Microplate Detection Platform
- SpectraMax® MiniMax™ 300 Imaging Cytometer
- SoftMax® Pro Software
|Parameter||Setting for cell counts|
|Optical configuration||SpectraMax MiniMax 300 Imaging Cytometer|
|Wavelength settings||Transmitted light|
|Image acquisition settings||Exposure: 8 ms|
|Image analysis settings||Analysis type: Discrete Object Analysis
Wavelength for finding objects: TL
|Find objects||Predefined setting: 'CellsB' or drawing|
About StainFree Cell Detection Technology
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.
The SpectraMax i3 Multi-Mode Microplate Platform with MiniMax 300 Imaging Cytometer uses unique, patent-pending StainFree Cell Detection Technology that allows you to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI, which intercalates with DNA, or live cell dyes that are actually toxic to cells in the long term.