The fibrosarcoma cell line HT1080 was generated in 1972 from tumor biopsy tissue. Interestingly, the patient had not been treated with radiation or chemotherapy at the time of biopsy, so the cells lack the sort of unwanted mutations that are commonly caused by these treatments. Because HT1080 cells are highly invasive, they are often used as models for cell invasion and migration. Researchers have been able to identify a number of potential therapeutic agents for cancer on the basis of their ability to inhibit the invasive behavior of HT1080 cells.
HT1080 Cells Analysis Toolkit
- SpectraMax® i3 Multi-Mode Microplate Detection Platform
- SpectraMax® MiniMax™ 300 Imaging Cytometer
- SoftMax® Pro Software
|Parameter||Setting for cell counts|
|Optical configuration||SpectraMax MiniMax 300 Imaging Cytometer|
|Wavelength settings||Transmitted light|
|Image acquisition settings||Exposure: 7 or 8 ms|
|Image analysis settings||Analysis type: Discrete Object Analysis
Wavelength for finding objects: TL
|Find objects||Setting: CellsA|
About StainFree Cell Detection Technology
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.
The SpectraMax i3 Multi-Mode Microplate Platform with MiniMax 300 Imaging Cytometer uses unique, patent-pending StainFree Cell Detection Technology that allows you to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI, which intercalates with DNA, or live cell dyes that are actually toxic to cells in the long term.