The epidermoid carcinoma cell line A431 expresses abnormally high levels of epidermal growth factor receptor (EGFR) and is a useful model for studying EGFR-mediated signaling, which regulates growth, survival, proliferation, and differentiation in mammalian cells. Derived from the epidermis of an 85-year old female patient and established by D. J. Giard et al., A431 cells have proven to be a valuable model system for examining the cell cycle, apoptosis, and cancer.
A431 cells have an epithelial morphology and tend to grow in clusters, making them a bit challenging to count. For StainFree counting, use ‘Create New Setting’ and use the yellow drawing tool to draw around the outlines of the cells without going outside the cell edges. Use the blue drawing tool to indicate non-cell areas and any artifacts.
A431 Cells Analysis Toolkit
- SpectraMax® i3 Multi-Mode Microplate Detection Platform
- SpectraMax® MiniMax™ 300 Imaging Cytometer
- SoftMax® Pro Software
|Parameter||Setting for cell counts|
|Optical configuration||SpectraMax MiniMax 300 Imaging Cytometer|
|Wavelength settings||Transmitted light (TL)
713 nm (red fluorescence)
|Image acquisition settings||TL exposure: 7 ms
TL Focus adjustment: 0 µm
|713 exposure: 12 ms
713 Focus adjustment: 20 µm
|Image analysis settings||Analysis type: Discrete Object Analysis
Wavelength for finding objects: TL (StainFree) or 713 (red nuclei)
|Find objects||TL: Create new setting (Custom)
713: Set Size and Intensity: Size = 10-30 µm, Intensity Above Background = 70
About StainFree Cell Detection Technology
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.
The SpectraMax i3/i3x Multi-Mode Microplate Platform with MiniMax 300 Imaging Cytometer uses unique, patent-pending StainFree Cell Detection Technology that allows you to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI, which intercalates with DNA, or live cell dyes that are actually toxic to cells in the long term.