Overview of SpectraMax MiniMax 300 Imaging Cytometer
See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity, cell proliferation, and protein expression. Now with StainFree™ Cell counting Technology, additional brightfield analysis features, and fluorescent green and red detection channels.
The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability.
- Simplified workflow: Add cellular imaging assays in a small footprint
- No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using StainFree™ Technology
- More data: Multi-parametric cell-based assays with brightfield and green and red fluorescent imaging
- Familiar software: Set up, image, and analyze cells in a snap using SoftMax® Pro Microplate Data Acquisition & Analysis Software
- Higher sensitivity: Easily measure low cell concentration samples
- Lower variance: Normalize your plate reader assay data to the number of cells in each well
Technologies of SpectraMax MiniMax 300 Imaging Cytometer
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Cell Count/Viability
- CHO cells were plated in 2X dilutions starting from 32000 cells/well in a 96-well plate
- Cells were then stained with Calcein AM dye and counted
Cell Count/Viability
- CHO cells were plated in 2X dilutions starting from 32000 cells/well in a 96-well plate
- Cells were then stained with Calcein AM dye and counted
Cell Proliferation/Cytotoxicity
- Hepatocytes were treated with known compounds causing liver toxicity
- Proliferation/toxicity effects assessed by measuring total cell area
Cell Proliferation/Cytotoxicity
- Hepatocytes were treated with known compounds causing liver toxicity
- Proliferation/toxicity effects assessed by measuring total cell area
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Marker Expression
- HUVEC cells were pre-treated with anti-inflammatory compounds and then cultured in the presence of inflammatory cytokines
- Stimulated protein expression was measured by total integrated intensity
Marker Expression
- HUVEC cells were pre-treated with anti-inflammatory compounds and then cultured in the presence of inflammatory cytokines
- Stimulated protein expression was measured by total integrated intensity
Specifications & Options of SpectraMax MiniMax 300 Imaging Cytometer
Resources of SpectraMax MiniMax 300 Imaging Cytometer
Application Note
Validate CRISPR-Edited Cells using Imaging and Western Blot Detection on a Microplate Reader
Validate CRISPR-Edited Cells using Imaging and Western Blot Detection on a Microplate Reader
Genome editing has been widely used to study gene expression and protein function, but many of these methods are labor intensive and inaccurate. The CRISPR (Clustered Regularly…
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Application Note
Acquire and analyze images of FUCCI spheroids on the SpectraMax MiniMax cytometer
Acquire and analyze images of FUCCI spheroids on the SpectraMax MiniMax cytometer
Spheroids are small three-dimensional (3D) cellular microenvironments grown using a variety of specialized culture methods such as low-adhesion microplates. This 3D cell culture…
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Application Note
Assay apoptosis in magnetically bioprinted HepG2 spheroids
Assay apoptosis in magnetically bioprinted HepG2 spheroids
Learn how HepG2 cells can be bioprinted using a magnetic 3D cell culture system and imaged on the SpectraMax i3x plate reader with MiniMax 300 Imager.
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Application Note
Improved cell counting method using transmitted light on the SpectraMax MiniMax cytometer
Improved cell counting method using transmitted light on the SpectraMax MiniMax cytometer
Hematoxylin, a natural product extracted from the heartwood of the logwood tree (Haematoxylin campechianum), is the most commonly used dye for staining protocols in histology.…
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Application Note
Evaluate cell migration with FluoroBlok inserts on the SpectraMax MiniMax cytometer
Evaluate cell migration with FluoroBlok inserts on the SpectraMax MiniMax cytometer
Cell migration, broadly defined as the movement of cells from one location to another, is important in diverse processes including embryonic development and wound healing. It is also a…
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Application Note
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite outgrowth and is regulated by…
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Application Note
Measuring marker expression with imaging cytometry on a plate reader
Measuring marker expression with imaging cytometry on a plate reader
The presence or absence of cellular proteins can indicate a reaction to stimulation, a state of differentiation, unique characteristics of a specific cell type, or success of a gene…
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Application Note
Image slide-based samples on the SpectraMax MiniMax cytometer
Image slide-based samples on the SpectraMax MiniMax cytometer
Biological samples ranging from bacterial smears to tissue cross sections are commonly studied on microscope slides. Using bright-field and fluorescence imaging, researchers can study…
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Application Note
Monitoring cytotoxicity on the SpectraMax i3 platform with MiniMax cytometer
Monitoring cytotoxicity on the SpectraMax i3 platform with MiniMax cytometer
Apoptosis is an important process in embryonic development as well as in cancer and neurodegenerative diseases. Assays for apoptosis and cell viability can provide information on the…
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Application Note
Assessing mitochondrial toxicity on the SpectraMax i3 Multi-Mode Detection Platform with SpectraMax MiniMax 300 Imaging Cytometer
Assessing mitochondrial toxicity on the SpectraMax i3 Multi-Mode Detection Platform with SpectraMax MiniMax 300 Imaging Cytometer
A key part of drug screening programs is monitoring effects of candidate drugs on mitochondrial health. Mitochondrial activity can be readily analyzed using fluorescent dyes available…
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Application Note
Wells to Westerns: Investigating the cellular heat shock response
Wells to Westerns: Investigating the cellular heat shock response
Investigating a cellular response can involve multiple approaches to gathering information—imaging, cell-based viability and proliferation assays, western blots to look at changes in…
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Application Note
Alternatives to DAPI staining: imaging and counting live cells
Alternatives to DAPI staining: imaging and counting live cells
DAPI (4’, 6-diamidino-2-phenylindole) is a fluorescent dye often used to stain nuclear DNA. It is employed in imaging experiments such as fluorescent microscopy, chromosome spreads,…
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Application Note
StainFree technology for cellular image analysis without fluorescent dyes
StainFree technology for cellular image analysis without fluorescent dyes
Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell…
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Application Note
Optimizing GFP transfection with the SpectraMax i3 Multi-Mode Detection Platform
Optimizing GFP transfection with the SpectraMax i3 Multi-Mode Detection Platform
Expression of fluorescent proteins, for example, green fluorescent proteins (GFP), is often used as a read-out for reporter gene assays. In a typical reporter assay, the regulatory…
Data Sheet
SpectraMax MiniMax 300 Imaging Cytometer
SpectraMax MiniMax 300 Imaging Cytometer
See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of…
eBook
Cellular Imaging Made Easy
Cellular Imaging Made Easy
A streamlined workflow for cell counting and phenotypic characterization is critical to many experiments. Our SpectraMax® i3x microplate reader with MiniMax™ cytometer provides you with…
Brochure
SpectraMax i3x Multi-Mode Detection Platform
SpectraMax i3x Multi-Mode Detection Platform
The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging…
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Brochure
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. Our…
Scientific Poster
Complex Cell Based Assays with a Novel Imaging Cytometry System and Object Recognition Software
Complex Cell Based Assays with a Novel Imaging Cytometry System and Object Recognition Software
Learn how to run complex cell-based assays while maintaining high-quality multi-parametric data output using a novel imaging cytometry system and object recognition software.
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Scientific Poster
HTRF® Technology on the SpectraMax® i3 Multi‐Mode Platform
HTRF® Technology on the SpectraMax® i3 Multi‐Mode Platform
Discover how the SpectraMax i3 Multi-mode platform can be used to run HTRF assays to study GPCRs, kinases, cell signaling, and more in this poster.
Videos and Demos
Cell-Based Assays Using a Novel Plate Reader Platform with Imaging Cytometry
SpectraMax i3x Multi-Mode Detection Platform
SpectraMax MiniMax 300 Imaging Cytometer
| Product Name | Description | Part Number |
| System ScanLater Western Blot Detection System |
|
0200-7027 |
| Assay Kit ScanLater: Eu-Streptavidin Evaluation Kit |
|
R8200
Click here to purchase |
| Assay Kit ScanLater: Eu-Streptavidin Bulk Kit |
|
R8203
Click here to purchase |
| Assay Kit ScanLater: Anti-Mouse Evaluation Kit |
|
R8201
Click here to purchase |
| Assay Kit ScanLater: Anti-Mouse Bulk Kit |
|
R8205
Click here to purchase |
| Assay Kit ScanLater: Anti-Rabbit Evaluation Kit |
|
R8202
Click here to purchase |
| Assay Kit ScanLater: Anti-Rabbit Bulk Kit |
|
R8204
Click here to purchase |
| Assay Kit ScanLater: Anti-Goat Evaluation Kit |
|
R8224
Click here to purchase |
| Assay Kit ScanLater: Anti-Goat Bulk Kit |
|
R8227
Click here to purchase |
| Assay Kit ScanLater: Anti-Rat Evaluation Kit |
|
R8225
Click here to purchase |
| Assay Kit ScanLater: Anti-Rat Bulk Kit |
|
R8228
Click here to purchase |
| Protein Ladder ScanLater Western Blot Protein Ladder |
|
R8220
Click here to purchase |
| Assay Kit Components | ||
| ScanLater Wash Buffer (100 mL) | R8206 | |
| ScanLater 5x Blocking Buffer (120 mL) | R8207 | |
| ScanLater Goat Anti-Mouse Eu-labeled Antibody (60 µg) | R8208 | |
| ScanLater Goat Anti-Rabbit Eu-labeled Antibody (60 µg) | R8209 | |
| ScanLater Eu-labeled Antibody Streptavidin (60 µg) | R8212 | |
| ScanLater Eu-Anti-Goat IgG (60 μg) | R8226 | |
Featured Applications
Cell Count/Viability
CHO cells were plated in 2X dilutions starting from 32000 cells/well in a 96-well plate Cells were…
Cell Proliferation/Cytotoxicity
Hepatocytes were treated with known compounds causing liver toxicity Proliferation/toxicity…
Marker Expression
HUVEC cells were pre-treated with anti-inflammatory compounds and then cultured in the presence of…
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