Soluble Periplasmic Production of Human Granulocyte Colony-stimulating Factor (G-CSF) in Pseudomonas fluorescens

Jin, H., et al., 78(1), 69-77, Protein Expression and Purification, 2011

To identify a strain producing high levels of intact, soluble Met-G-CSF product, more than 150 protease defective host strains from the Pfenex Expression Technology™ toolbox were screened in parallel using the Octet system to quantify active GCSF binding to its receptor. For these measurements, recombinant GCSF receptor was biotinylated and immobilized on Streptavidin biosensors.

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