- Clone Screening
- Single-Cell Isolation
- DispenCell Single-Cell Dispenser
DispenCell Single-Cell Dispenser
Compact, automated cell dispenser for fast, easy and gentle single-cell isolation
The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument designed for fast, easy and gentle single-cell isolation. DispenCell integrates seamlessly into your laboratory workflow, with a plug-and-play approach. Flexible and effortless, DispenCell operates equally under sterile conditions in a culture hood, or on a simple benchtop.
DispenSoft, the single-cell analysis software included with DispenCell, provides immediate and traceable proof of clonality. The instrument is fitted with a sensing tip that detects the passage of cells, allowing users to check for proof of clonality immediately after the cells are dispensed. All dispensing data is automatically stored, allowing for easy generation of a clonality report.
DispenCell has unique technology which allows for extremely gentle handling of the cell sample. Cells are exposed to no more pressure than with manual pipetting (less than 0.1 psi). This allows the instrument to preserve cell viability and outgrowth while simultaneously increasing cell deposition efficiency.
The small footprint of the DispenCell allows it to fit perfectly into any laboratory setting: under a hood to work under sterile conditions, on a bench top for routine seeding, or in a pre-existing automated workflow.
Introducing DispenCell Single-Cell Dispenser
A single-cell analysis software tool provides a traceable proof of clonality report instantly.
A patented disposable tip ensures clean isolation of single cells and no cross contamination. Certified free from animal products and cytotoxic material.
Unique design ensures gentle dispensing for better viability and cloning efficiency
Easy to set up, and intuitive with a simple interface. No cleaning or calibration required.
The all-new CloneSelect Imager FL adds high contrast multichannel fluorescent technology in addition to the standard white light imaging that allows for accurate single-cell detection and proof of monoclonality at day 0. Identify and verify gene edits with comparative confluence assays.
Technology enables scientists to isolate single cell lines three times faster and at lower cost compared to existing solutions SAN JOSE, Calif., Feb. 7, 2023 – Molecular…
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
The isolation of single cells remains a challenging task in single-cell genomics. Current methods lack the evidence that only a single-cell has been isolated in the analysis vessel. It is important that the integrity of the cells is maintained prior to their lysis in order to preserve their DNA.
Furthermore, cells should undergo as little stress as possible prior to their lysis in order to preserve their RNA and its expression level. The CloneSelect Single-Cell Printer Series deposits single cells in a very gentle manner, guaranteeing high purity and high viability.
This provides optimal basis for downstream single-cell genomic analysis.
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming. The DispenCell™ Single-Cell Dispenser enables fast, easy, and gentle isolation of cells, as well as provides instantaneous proof of clonality and traceability post-cell dispensing.
** Monoclonal reliability error definition:
Impedance trace reliability error = P(obs > 1|z = 1) = P(obs > 1nz = 1)/P(z = 1)
The monoclonal reliability error is defined as the probability of observing more than one particle/cell when the impedance time trace exhibits one event. The monoclonal reliability is defined as the ones' complement of its error.
Single-cell RNA sequencing (scRNA-seq) is a state of the art technology for the unbiased characterization of gene expression in complex cell and tissue samples. Despite having…
Technology enables scientists to isolate single cell lines three times faster and at lower cost compared to existing solutions SAN JOSE, Calif., Feb. 7, 2023 – Molecular Devices, LLC…
DispenCell™ is an automated laboratory instrument developed for fast, easy and gentle single-cell isolation. Designed by scientists for scientists, DispenCell has been designed to integ…
In April 2021, we launched our Organoid Innovation Centre (OIC), an initiative designed to help scientists leverage the potential of 3D biology throughout the drug discovery process.…
Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
Colony picking represents a powerful tool within the workflow of mammalian cell line development and the study of microbiology.
Discover why mAbs are key in the fight against SARS-CoV-2 and how the pandemic has shaped their discovery and development pipeline. Over the past three years, the development of…
Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19. Join us as we explore a scientific timeline of key…
Monoclonal assurance is undeniably critical in cell line development for generating therapeutics, such as monoclonal antibodies or cell-based therapies. To utilize a cell line for…
With solutions from Molecular Devices, scientists can fast-track the FDA approval process and accelerate monoclonal antibody discovery The timeline to identify and develop…
Advance your microbial and mammalian clone screening with proven, automated technologies
The market for biotherapeutics and therapeutic proteins was valued at $93.14 billion in 2018 and is expected to nearly double by the end of 2022.
Since the first approval of recombinant insulin and human growth hormone in the early 1980s, a multitude of recombinant protein therapeutics have been approved by regulatory…
Double confirmation of clonality. Image based evidence confirming single cell deposition event, and single cell in 96- and 384-well plate post sort
Dwayne Carter gives us a taste of 3D bioprinting, clone screening, and Caribbean cuisine Dwayne Carter is a cell biologist and educator who joined Molecular Devices in November 2020…
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is deri…
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is…
In this app note, we demonstrate a unified workflow that combines single cell deposition and clonality verification. We couple the use of the SCP and CSI to deposit single cells into a…
Cell line development is a crucial step in upstream processes for generating biopharmaceutical molecules like monoclonal antibodies. Traditional workflows in cell line development ha…
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Molecular Devices offer products and unrivaled solutions that utilize imaging & intelligent image analysis to support basic research, pharmaceutical & biotherapeutic developm…
Introducing DispenCell Single-Cell Dispenser
DispenCell Cell Sample Prep Tutorial
DispenCell Tip Installation
Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow - AstraZeneca
Stable Cell Line Development Workflow
Immunology and Vaccine Development Workflow
The many and varied workflows of cell line development
f.Sight vs. FACS and LD overview
Optimized workflow for rapid identification of neutralizing antibodies against viral particles
A Microfluidics-Based Single Cell Isolation Workflow Optimized for Efficiency, Viability, and Assurance of Monoclonality