Hybridoma Workflow for Antibody Discovery
Hybridoma generation and screening of large antibody libraries
Hybridoma technology is a method for mass-producing antibodies in a hybrid cell line generated from the fusion of antibody-producing B-cells with an immortalized myeloma cell line, now called a hybridoma cell. Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to generate a diverse library of unique monoclonal antibodies at a large scale, which are very frequently used in the prevention, diagnosis, and treatment of disease.
In this video, Justin Dranschak, manager for BioPharma platforms, presents our solution for a hybridoma workflow and references the systems to aid in your research.
Step 1: Fusion
The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma.
Step 2: Clone screening
The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies.
Step 3: Cell growth
Cell growth is determined by monitoring cell divisions over a given period of time using label-free imaging.
Step 4: Specificity and cross reactivity
The process of analyzing the ability of an antibody to bind to only one target antigen.
Step 5: Binding affinity and internalization
Binding affinity is the strength of the binding interaction between a single biomolecule (e.g. protein or DNA) to its ligand/binding partner (e.g. drug or inhibitor). Internalization is the process of monitoring the ability of a given particle to enter into the cell.
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ImageXpress® Cellular Imaging Systems
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SpectraMax® Microplate Reader and SoftMax® Pro GxP Compliance Software
Our SpectraMax® series of microplate readers measure absorbance, fluorescence, and luminescence plus additional detection modes with available upgrades for western blot, cell imaging, and fast kinetics with injectors. GMP/GLP labs can meet FDA guidelines with our SoftMax Pro GxP compliance and validation solution.
SoftMax® Pro 7.1.1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Every step is optimized to simplify analysis and reporting to support our microplate readers.
QPix™ Microbial Colony Picker
The QPix™ 400 Series Microbial Colony Pickers combine intelligent image analysis with precise automation for fast and efficient screening of large libraries. Capable of picking up to 3000 colonies per hour, it will streamline your workflow.
The ClonePix™ 2 Mammalian Colony Picker is a fully automated system for the selection of high-value clones used in antibody discovery and cell line development.
CloneSelect™ Single-Cell Printer™ Series
The CloneSelect™ Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge.
The CloneSelect™ Imager is a high-throughput automated solution for imaging and analyzing mammalian cells.
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Resources related to COVID-19 cellular responses and vaccine development
Systems to accelerate your COVID-19 cellular response and vaccine development
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