COVID-19 RESPONSE - We are committed to supporting our scientific community during this pandemic. Learn more 

A complete solution for the automated screening and objective selection of high-value clones across diverse cell types.

 

The ClonePix® 2 Mammalian Colony Picker is a fully automated system for selecting high-value clones used in antibody discovery and cell line development. Screen more clones in less time with monoclonal verification on day zero, then screen and identify for highest producers in weeks, not months.

Hybridomas, CHO cells, stem cells and other cell types are imaged and selected based on user-defined parameters. Plate handling, barcode reading, and picking are fully integrated, and all data, including images, are saved for downstream analysis. The picker increases the probability of finding optimally produced cell lines and significantly reduces time and labor.

  • Efficient Icon

    Automate antibody discovery and cell line development workflows

    ClonePix 2 picker is 10x faster than labor-intensive limiting dilution and FACS. Our sophisticated software and integrated robotics enable a screening speed of > 10,000 clones per day.

  • Select Icon

    Select cells with desirable attributes with monoclonality assurance from day 0

    Easily screen and select clones based on protein productivity, antigen-specificity, cell viability, and expression levels of tagged recombinant proteins.

  • Accuracy Icon

    Increase the probability of identifying high-value clones while eliminating or recovering unstable clones early

    Picking accuracy < 1 mm. Robotic picking reduces the risk of colony disturbance. Images of picked clones are stored with data.

ClonePix 2

ClonePix 2

Features

  • Breadth Icon

    Multiple detection methods

    White light identifies and measures clone morphology, size and proximity. Fluorescence indicates expression level and/or specificity. Up to five fluorescent filters are available for multiplexing.

  • Sterility Icon

    Sterility maintenance

    A host of sterility features and options including a UV light process for sanitizing the interior of the instrument, as well as pin washing and halogen drying are standard.

  • Connectivity Icon

    Integrated plate storage

    Includes two storage stacks for source and destination plates, each with a capacity of 10 plates.

  • Picks Icon

    Discrete colony formation

    Semi-solid CloneMediaTM encourages single cells to grow into discreet colonies, and allows for ease of plating. The media allows for a higher density of clones to be screened.

  • Efficient Icon

    Animal-free media and reagents

    Chemically defined and animal-free, CloneMedia cell-culture media, is optimized to increase productivity and aid in visualizing secreted antibodies when used with the CloneDetectTM detection agent.

  • Automation Icon

    Custom automation options*

    The Advanced Workflow Engineering Solutions Team can customize the monoclonality system and offer added services such as integrated verification of monoclonality.

*Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.
 

Redefine clone screening and selection with transformative cell line development workflows

Empower your team with data analysis that automatically generates a map of clones and their secretion levels from a series of images generated in situ. The ClonePix can also be customized to include image-based monoclonality assurance on day zero.* This means your team can screen in one round and then pick for the highest produces in weeks, not months.

 

Redefine clone screening and selection

Data analysis and tracking—reveal stable clones faster

Data analysis and tracking

Data Analysis

  • Automatically generate a 2D map of clones and their secretion levels from a series of images generated in situ
  • Screen and select colonies based on:
    • – Size, roundness, and proximity to neighbors
    • – Ranking according to fluorescence levels
    • – Closely placed colonies ignored via user-controlled “proximity” software setting

 

Data Tracking

All relevant data associated with each colony (including images taken before and after picking along with their picking coordinates) are automatically saved for review and downstream analysis.

Download brochure

Enhanced with monoclonality assurance*

Same ClonePix workflow, now enhanced with high-resolution single-cell imaging capability on day 0

Stable cell line development workflow

The enhanced ClonePix 2 system can automatically screen and pick clones that are both high producing and monoclonal—all in one system. Screen more clones in less time with monoclonal verification on day zero, then screen and pick for highest produces in less than two weeks.

  • Reduce screening time from two rounds to one by providing image-based evidence of clonality
  • Rapid Z-stack acquisition feature allows detection of single cells throughout the medium volume, not just a single focal plane, on day 0
  • Simplified workflow from single-cell identification and productivity screening with the all-in-one system

Download brochure

Enhanced for stem cell applications*

Identify desirable, clonal stem cell colonies for high-throughput colony screening and picking

Enhanced for stem cell applicationsstem cell picking pins

High-resolution imaging identifies desirable, clonal stem cell colonies for high-throughput colony screening and picking. Specialized picking pins allow the gentle transfer of adherent, feeder-free cells to high-density plates for clonal expansion and downstream analysis.

  • Colony formation - Individual stem cells plated at a low density in 6-well plates divide and develop into colonies. Plating density is kept low to ensure that colonies are derived from a single parent cell.
  • Clone screening - Clonally-derived stem cell colonies are identified by their desirable morphological characteristics and are picked from the low-density 6-well plates into higher density 96-well plates to carry forward with the screening. The ClonePix® 2 Mammalian Colony Picker can be customized with high-resolution optics and stem cell-specific pins, allowing this established technology to be leveraged in stem cell workflows.
  • Cell growth - Cell growth is determined by monitoring cell division over a given time using label-free imaging.
  • Functional screening - In addition to monitoring growth, functional cell-based assays can be performed. This can include assays assessing differentiation potential, pluripotency, ability to form 3D organoids, and other desirable traits.

Download brochure

 

*Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause an adjustment to standard performance.

Custom solutions are subject to Molecular Devices Custom Products Purchase Terms.

Latest Resources

44

Applications of ClonePix 2 Mammalian Colony Picker

  • Cell Line Development

    Cell Line Development for Recombinant Proteins

    Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.

    Download ebook 

    Cell surface expression screening

    Cell surface expression screening

    Many proteins that express to the surface of cells are targets for the discovery and development of biopharmaceuticals. For instance, G-protein coupled receptors (GPCRs) are the largest class of cell-surface proteins and are targets for almost 40% of existing drugs. Discovery and selection of high value clones with elevated cell surface expression of GPCRs from a transfected pool of cells can be challenging. The ClonePix 2 System represents an automated method of screening large populations of cells that increases the probability of finding rare high-affinity binder or high producer.

  • Clone productivity screening and titer

    Clone productivity screening and titer

    An important component in identifying high-value clones is determining productivity of single cell-derived colonies. Screening for productivity using traditional approaches is laborious and time consuming, generally consisting of a multistep process that involves isolating single cells from limiting dilution followed by assessment of titer using ELISA. The ClonePix 2 system combines phenotype selection, single-cell isolation and productivity screening into a single step, resulting in dramatically shorter screening times and increased number of candidates.

    Hybridoma Screening

    Hybridoma Screening

    Antibody discovery typically refers to the screening and identification of monoclonal antibodies (mAbs) that target a specific epitope for the diagnosis and treatment of diseases. A common approach to generating monoclonal antibodies involves the fusion of a pre-mitotic cancer cell with a post-mitotic and terminal antibody-expressing B-cell from the spleen. The resulting fused cell is called a hybridoma and has the advantage of producing mAbs while dividing to regenerate itself. Screening hybridomas for binding specificity or productivity can be automated using the ClonePix 2 System.

    Learn more 

  • Monoclonal Antibody Discovery

    Antigen-Specific Screening

    Antibody discovery typically refers to the screening and identification of specific antibodies that target an antigen molecule for the diagnosis and treatment of diseases. The specificity of the antibody is based on its ability to bind the epitope, a unique region on the antigen molecule. Therapeutic antibodies are typically monoclonal, single cell-derived and target a unique epitope region on the antigen. The ClonePix 2 System automates screening and rapid detection of antigen-specific clones from a heterogenous population of cells.

    Learn more  

    Monoclonality

    Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.

    Learn more 

Specifications & Options of ClonePix 2 Mammalian Colony Picker

Resources of ClonePix 2 Mammalian Colony Picker

Presentations
Videos & Webinars
Stable Cell Line Development Generation

Stable Cell Line Development Workflow

Hybridoma Workflow

Hybridoma Workflow

mammalian cells secreting non-mAb proteins workflow

Selection of high producing mammalian cells secreting non-mAb proteins workflow - SynBioBeta Lightning Talk

Rapid identification of Neutralizing Antibodies

Optimized workflow for rapid identification of neutralizing antibodies against viral particles

Selection GPCR Cell Line

Identification and Selection of GPCR Cell Lines with ClonePix 2

ClonePix 2 System

ClonePix 2

  • Citation
    Dated: Jan 01, 2022
    Publication Name: Cancer Immunoprevention - Methods in Molecular Biology

    Monoclonal Antibodies Generation: Updates and Protocols on Hybridoma Technology

    Since its inception in 1975, the hybridoma technology revolutionized science and medicine, facilitating discoveries in almost any field from the laboratory to the clinic. Many technological advancements have been developed since then, to create these “magical bullets.” Phage and yeast display libraries expressing the variable heavy and light… View more

    Since its inception in 1975, the hybridoma technology revolutionized science and medicine, facilitating discoveries in almost any field from the laboratory to the clinic. Many technological advancements have been developed since then, to create these “magical bullets.” Phage and yeast display libraries expressing the variable heavy and light domains of antibodies, single B-cell cloning from immunized animals of different species including humans or in silico approaches, all have rendered a myriad of newly developed antibodies or improved design of existing ones. However, still the majority of these antibodies or their recombinant versions are from hybridoma origin, a preferred methodology that trespass species barriers, due to the preservation of the natural functions of immune cells in producing the humoral response: antigen specific immunoglobulins. Remarkably, this methodology can be reproduced in small laboratories without the need of sophisticate equipment. In this chapter, we will describe the most recent methods utilized by our Monoclonal Antibodies Core Facility at the University of Texas–M.D. Anderson Cancer Center. During the last 10 years, the methods, techniques, and expertise implemented in our core had generated more than 350 antibodies for various applications.

    Contributors: Ahmed Muhsin, Roberto Rangel, Long Vien, Laura Bover  
    Go to article

  • Citation
    Dated: Dec 01, 2017
    Publication Name: Journal of Immunological Methods

    Sequential screening by ClonePix FL and intracellular staining facilitate isolation of high producer cell lines for monoclonal antibody manufacturing

    Screening and characterization of cell lines for stable production are critical tasks in identifying suitable recombinant cell lines for the manufacture of protein therapeutics. To aid this essential function we have developed a methodology for the selection of antibody expressing cells using fluorescence based ClonePix FL colony isolation and… View more

    Screening and characterization of cell lines for stable production are critical tasks in identifying suitable recombinant cell lines for the manufacture of protein therapeutics. To aid this essential function we have developed a methodology for the selection of antibody expressing cells using fluorescence based ClonePix FL colony isolation and flow cytometry analysis following intracellular staining for immunoglobulin G (IgG). Our data show that characterization of cells by flow cytometry early in the clone selection process enables the identification of cell lines with the potential for high productivity and helps to eliminate unstable cell lines. We further demonstrate a correlation between specific productivity (qP) and intracellular heavy chain (HC) content with final productivity. The unique combination of screening using ClonePix FL and the flow cytometry approaches facilitated more efficient isolation of clonal cell lines with high productivity within a 15 week timeline and which can be applied across NS0 and CHO host platforms. Furthermore, in this study we describe the critical parameters for the ClonePix FL colony based selection and the associated calculations to provide an assessment of the probability of monoclonality of the resulting cell lines.

    Contributors: Gargi Roya, Guillermo Miro-Quesadab, Li Zhuanga, Tom Martina, Jie Zhub, Herren Wua, Marcello Marellia, Michael A.Bowena  
    Go to article

  • Citation
    Dated: Dec 14, 2015
    Publication Name: 24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products

    CHO-DHFR cell line development platform: Application of Clonepix and Automated Mini Bioreactor (AMBR) technologies to meet accelerated timelines

    The Holy Grail sought by all Bioprocess Cell Line Development (CLD) groups is achieving high yields from easily-cultured, robustly-growing cells in timelines measured in weeks rather than months. As the first bottleneck in process development, CLD must first birth its product for upstream and downstream groups to initiate their own reproductive… View more

    The Holy Grail sought by all Bioprocess Cell Line Development (CLD) groups is achieving high yields from easily-cultured, robustly-growing cells in timelines measured in weeks rather than months. As the first bottleneck in process development, CLD must first birth its product for upstream and downstream groups to initiate their own reproductive cycles. To facilitate shortened CLD timelines, scientists have turned to new technologies and automation platforms. Emerging high-throughput instrumentation such as Clonepix and Automated MicroBioreactors (AMBR) have been enthusiastically integrated into stable cell line generation platforms; however, application of these methodologies among users is divergent.

    Contributors: Venkata R Mangalampalli, Dyane Wycuff, Mingzhong Chen, David Berlinger, Elizabeth H Scheideman, Amritha Menon, Guilia Fabozzi, Althaf Hussain & Richard M Schwartz  
    Go to article

  • Citation
    Dated: May 25, 2014
    Publication Name: New Biotechnology

    High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system

    Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high-expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to genetic instability, variable expression, product heterogeneity and prolonged development… View more

    Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high-expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to genetic instability, variable expression, product heterogeneity and prolonged development timelines. Inclusion of cis-acting ubiquitous chromatin opening elements (UCOE™) in mammalian expression vectors has been shown to improve productivity and facilitate high-level gene expression irrespective of the chromosomal integration site without lengthy gene amplification protocols. In this study we have used high-throughput robotic clone selection in combination with UCOE™ containing expression vectors to develop a rapid, streamlined approach for early-stage cell line development and isolation of high-expressing clones for mAb production using Chinese hamster ovary (CHO) cells. Our results demonstrate that it is possible to go from transfection to stable clones in only 4 weeks, while achieving specific productivities exceeding 20 pg/cell/day. Furthermore, we have used this approach to quickly screen several process-crucial parameters including IgG subtype, enhancer-promoter combination and UCOE™ length. The use of UCOE™-containing vectors in combination with automated robotic selection provides a rapid method for the selection of stable, high-expressing clones.

    Contributors: Jeff Jia Cheng Hou, Ben S. Hughes, Matthew Smede, Kar Man Leung, Kara Levine, Susan Rigby, Peter P. Gray, Trent P.Munro  
    Go to article