Mammalian and microbial clone screening projects traditionally begin with a target—a receptor, protein, or gene that participates in a biological pathway of interest. Next comes screening, where thousands to millions of cells are tested and analyzed in relation to the target. This presents a significant bottleneck for labs as it requires laborious and time-consuming methods across a wide range of analytical platforms.
With Molecular Devices automated clone screening workflows, you can ease the burden on your lab by significantly reducing hands-on-time while creating a central repository for data pulled from multiple processes. Our automated solutions unify all of your laboratory devices to increase your throughput and efficiency while reducing human interaction.
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones are then characterized before final selection and scale-up.
Molecular cloning refers to the isolation of a DNA sequence from any species (often a gene), and its insertion into a vector for propagation, without alteration of the original DNA sequence. This is a very laborious process when done manually requiring hundreds of plates and kits. There are several areas where errors or contamination can occur. Automating the process can reduce FTE time by 50% while increasing throughput and efficiency two fold.
Cell cultured meat is a product of cellular agriculture that uses biopsied animal cells to grow sustainable, humane meat as an alternative to the traditional farming industry. Because cell cultured meat is made with mammalian cells, many of the processes in developing and scaling a cell cultured meat product will mirror those in in biologics production, specifically cell line development.
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Gene editing is a genetic manipulation in which a living organism’s genomic DNA is deleted, inserted, replaced, or modified. Gene editing is a site-specific targeting to create breaks in DNA through various techniques and does not always involve repair mechanisms. It consists of two techniques – inactivation and correction.
Antibody discovery typically refers to the screening and identification of monoclonal antibodies (mAbs) that target a specific epitope for the diagnosis and treatment of diseases. A common approach to generating monoclonal antibodies involves the fusion of a pre-mitotic cancer cell with a post-mitotic and terminal antibody-expressing B-cell from the spleen. The resulting fused cell is called a hybridoma and has the advantage of producing mAbs while dividing to regenerate itself. Screening hybridomas for binding specificity or productivity can be automated using the ClonePix 2 System.
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Phage Display is a technique to enable the study of protein, peptide or DNA interaction with a target protein. This molecular tool enables the discovery of high-affinity binders by using bacteriophages to present a target protein on the exterior of the viral coat, while containing the DNA encoding the target protein inside the viral coat. The resultant displaying phages can be screened for binding against a library of peptides or proteins in a high throughput fashion. QPix colony pickers can be used to automate inoculation, plating, spreading and picking in a Phage Display workflow.
Cellular agriculture allows us to produce genuine animal proteins through microbial precision fermentation. Using synthetic biology techniques like strain engineering, scientists can engineer microbial cells as cellular factories for various organic molecules, most commonly proteins. In the food supply chain, precision fermentation is used to create everything from plant-based proteins from yeast cells to dairy- and animal-free whey protein from fungal strains.
Synthetic biology is a broad term that refers to the manipulation of genetic pathways to harness the power of existing biological systems in novel ways (often to manufacture molecules or proteins). Synthetic biology applies principles that are derived from engineering, specifically design-build-test-learn cycles, to biological systems. By leveraging high-throughput workflows, synthetic biologists can accelerate this process.
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.
Application Note
An increase in demands for faster high-throughput cell-line development and better screening in the pharmaceutical space is driving research facilities and companies to automate…
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SLAS Europe 2022, hosted numerous sessions packed with the latest research on emerging topics as well as sessions and panel discussions focused on how to build, and succeed, in a…
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Larger site will be future home of the Organoid Innovation Center – Salzburg, a collaborative space for advancing automated cell line development, organoid development, and screening…
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Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
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One of the biggest global concerns is our excessive use of resources and its undeniable impact on the environment. In particular, manufacturing processes require enormous amounts of…
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Discover why mAbs are key in the fight against SARS-CoV-2 and how the pandemic has shaped their discovery and development pipeline. Over the past three years, the development of…
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Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19. Join us as we explore a scientific timeline of key…
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For three decades, Molecular Devices has made scientific breakthroughs possible for academic, pharmaceutical, government, and biotech customers with its innovative life science…
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SLAS2022, the Society for Lab Automation and Screening conference offered another exciting year for learning about innovative laboratory technologies. Whether you attended in-person…
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Monoclonal assurance is undeniably critical in cell line development for generating therapeutics, such as monoclonal antibodies or cell-based therapies. To utilize a cell line for…
Scientific Poster
3D cell culture as a model system is increasingly popular because it recapitulates the in vivo microenvironment better than 2D cell cultures. Organoids have the capacity for stable d…
Scientific Poster
Triple negative breast cancer is a clinically aggressive tumor subtype, with high rates of metastasis, recurrence, and drug resistance. Currently there are no clinically approved sma…
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In life sciences, more is more. Obtaining a great body of data that is consistent and insightful to get a qualitative and quantitative overview of your model is critical. However,…
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With solutions from Molecular Devices, scientists can fast-track the FDA approval process and accelerate monoclonal antibody discovery The timeline to identify and develop…
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Advance your microbial and mammalian clone screening with proven, automated technologies
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Lab Manager speaks to Dan O’Connor, vice president, drug discovery, Molecular Devices, about the company’s Organoid Innovation Center in San Jose, CA. The center is 180 sq. ft., with…
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The market for biotherapeutics and therapeutic proteins was valued at $93.14 billion in 2018 and is expected to nearly double by the end of 2022.
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QPix™ systems earned a well-deserved reputation for performance and reliability during the race to sequence the human genome with the Human Genome Project, and continues to support i…
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During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
Scientific Poster
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is deri…
Application Note
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is…
Application Note
In this app note, we demonstrate a unified workflow that combines single cell deposition and clonality verification. We couple the use of the SCP and CSI to deposit single cells into a…
Application Note
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
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The QPix™ Chroma Filter is a thin film translucent optical filter that provides a robust method to confidently select and pick rare expressing bacterial colonies based on color inten…
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The Edinburgh Genome Foundry (EGF) is dedicated to automated design and assembly of large DNA constructs using a fully automated robotic platform. They needed an automated colony picker…
Application Note
Cell line development requires screening tens of thousands of clones in order to find the few clones that are stable, grow well, and produce high yields of bioproduct. In addition to…
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CloneSelect Imager system produces objective, quantitative, and consistent results in less time to overcome the challenges associated with conventional cell confluence techniques.…
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Explore PDF to learn how you can accelerate your cell line development and get to your high value hits faster.
Automating culture and high-content imaging of 3D organoids for in vitro assessment of compound effects
Tips to automating molecular cloning and strain engineering applications
Advanced Workflow Engineering Solutions (AWES) Introductory
Customization and Automation
Cell cultured meat is a product of cellular agriculture that uses biopsied animal cells to grow…
Cell line development is a critical step in the process of generating biopharmaceutical molecules,…
Gene editing is a genetic manipulation in which a living organism’s genomic DNA is deleted,…
Antibody discovery typically refers to the screening and identification of monoclonal antibodies (…
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single…
Phage Display is a technique to enable the study of protein, peptide or DNA interaction with a…
Cellular agriculture allows us to produce genuine animal proteins through microbial precision…
Synthetic biology is a broad term that refers to the manipulation of genetic pathways to harness…
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It…
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