Lab automation for high-throughput clone screening
Fully-integrated, lab automation solution for molecular cloning, antibody discovery, and monoclonality
Explore automation-ready systems for your clone screening workflow
Mammalian and microbial clone screening projects traditionally begin with a target—a receptor, protein, or gene that participates in a biological pathway of interest. Next comes screening, where thousands to millions of cells are tested and analyzed in relation to the target. This presents a significant bottleneck for labs as it requires laborious and time-consuming methods across a wide range of analytical platforms.
With Molecular Devices automated clone screening workflows, you can ease the burden on your lab by significantly reducing hands-on-time while creating a central repository for data pulled from multiple processes. Our automated solutions unify all of your laboratory devices to increase your throughput and efficiency while reducing human interaction.
Automated cell line development workflow
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones are then characterized before final selection and scale-up.
Workcell integrated system solution:
- CloneSelect Single-Cell Printer
- CloneSelect Imager
- SpectraMax iD5 Multi-Mode Microplate Reader
- AquaMax Microplate Washer
- Sartorius Octet BLI Label-Free Detection Systems
- Beckman Coulter Biomek automated liquid handler
- Bionex Solutions HiG4 automated centrifuge
- LiCONiC LiCotel automated plate hotels
- LiCONiC StoreX automated incubator
Automated molecular cloning workflow
Molecular cloning refers to the isolation of a DNA sequence from any species (often a gene), and its insertion into a vector for propagation, without alteration of the original DNA sequence. This is a very laborious process when done manually requiring hundreds of plates and kits. There are several areas where errors or contamination can occur. Automating the process can reduce FTE time by 50% while increasing throughput and efficiency two fold.
Workcell integrated system solution:
- QPix Microbial Colony Pickers
- SpectraMax Microplate Reader with SoftMax Pro GxP Software
- Beckman Coulter Biomek automated liquid handler
- LiCONiC StoreX automated incubator
- Micronic Tube Capper/De-Capper
- Plate labeler, sealer and peeler
- Multidrop Combi
- PCR Platform
- Plate Centrifuge
- Fragment analyzer
Featured Resources
Resources of Lab automation for high throughput clone screening
Application Note
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
In this app note, we demonstrate a unified workflow that combines single cell deposition and clonality verification. We couple the use of the SCP and CSI to deposit single cells into a…
Brochure
CloneSelect Imager
CloneSelect Imager
CloneSelect Imager system produces objective, quantitative, and consistent results in less time to overcome the challenges associated with conventional cell confluence techniques.…
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Brochure
Accelerate Cell Line Development
Accelerate Cell Line Development
Explore PDF to learn how you can accelerate your cell line development and get to your high value hits faster.
Blog
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
Monoclonal assurance is undeniably critical in cell line development for generating therapeutics, such as monoclonal antibodies or cell-based therapies. To utilize a cell line for…
Scientific Poster
Automation of 3D organoid culture workflow with deep-learning based image analysis
Automation of 3D organoid culture workflow with deep-learning based image analysis
3D cell culture as a model system is increasingly popular because it recapitulates the in vivo microenvironment better than 2D cell cultures. Organoids have the capacity for stable d…
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Scientific Poster
Automation and high content imaging of 3D triple-negative breast cancer patient-derived
Automation and high content imaging of 3D triple-negative breast cancer patient-derived
Triple negative breast cancer is a clinically aggressive tumor subtype, with high rates of metastasis, recurrence, and drug resistance. Currently there are no clinically approved sma…
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Blog
Lab Automation 101: Inside Access From Our Subject Matter Expert
Lab Automation 101: Inside Access From Our Subject Matter Expert
In life sciences, more is more. Obtaining a great body of data that is consistent and insightful to get a qualitative and quantitative overview of your model is critical. However,…
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Blog
Accelerated Production of Monoclonal Antibodies for the Treatment of SARS-CoV-2
Accelerated Production of Monoclonal Antibodies for the Treatment of SARS-CoV-2
With solutions from Molecular Devices, scientists can fast-track the FDA approval process and accelerate monoclonal antibody discovery The timeline to identify and develop…
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Brochure
Clone screening solutions
Clone screening solutions
Advance your microbial and mammalian clone screening with proven, automated technologies
Publications
Project Profile: Organoid Innovation Center
Project Profile: Organoid Innovation Center
Lab Manager speaks to Dan O’Connor, vice president, drug discovery, Molecular Devices, about the company’s Organoid Innovation Center in San Jose, CA. The center is 180 sq. ft., with…
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Application Note
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
The market for biotherapeutics and therapeutic proteins was valued at $93.14 billion in 2018 and is expected to nearly double by the end of 2022.
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Brochure
QPix colony picking series
QPix colony picking series
QPix™ systems earned a well-deserved reputation for performance and reliability during the race to sequence the human genome with the Human Genome Project, and continues to support i…
eBook
Optimize your high-value cell lines
Optimize your high-value cell lines
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
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Scientific Poster
A comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for mAb production
A comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for mAb production
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is deri…
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Application Note
Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production
Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is…
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Application Note
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
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Publications
QPix™ Chroma Filter Thin Film Optical Filter for Blue/White Colony Selection
QPix™ Chroma Filter Thin Film Optical Filter for Blue/White Colony Selection
The QPix™ Chroma Filter is a thin film translucent optical filter that provides a robust method to confidently select and pick rare expressing bacterial colonies based on color inten…
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Brochure
QPix Custom Microbial Colony Pickers
QPix Custom Microbial Colony Pickers
The Edinburgh Genome Foundry (EGF) is dedicated to automated design and assembly of large DNA constructs using a fully automated robotic platform. They needed an automated colony picker…
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Application Note
Improved productivity of cell line development using fully integrated automated solutions: a series of case studies with the CloneSelect Imager
Improved productivity of cell line development using fully integrated automated solutions: a series of case studies with the CloneSelect Imager
Cell line development requires screening tens of thousands of clones in order to find the few clones that are stable, grow well, and produce high yields of bioproduct. In addition to…
Automating culture and high-content imaging of 3D organoids for in vitro assessment of compound effects
Tips to automating molecular cloning and strain engineering applications
Advanced Workflow Engineering Solutions (AWES) Introductory
Customization and Automation
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