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Label-free imaging solution for assurance of monoclonality

The CloneSelect® Imager is a high-throughput automated solution for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provide accurate, objective, and consistent results. Detailed imaging and comprehensive data analysis enable better decision making. With the ability to image a 96-well plate in 90 seconds, the imager will increase throughput and productivity.

  • Validation Icon

    Verify monoclonality easily

    The Monoclonality Report feature streamlines the creation of supporting documentation for regulatory agencies. Reports are automatically generated based on parameters you select.

  • Accuracy Icon

    Detect cells accurately

    Algorithms are optimized for accurate cell detection and address varying cell types and conditions. High resolution imaging provides accuracy and assurance of monoclonality.

  • Efficient Icon

    Screen more clones in less time

    The imager delivers industry leading acquisition times, allowing for imaging a 96-well plate in as little at 90 seconds.

Features

  • Customizabl Icon

    Choice of imaging modes

    Label-free brightfield provides rapid acquisition without the use of harmful fluorescent agents. Customizable fluorescence imaging provides additional confidence of monoclonality.

  • Data Icon

    Autogenerated data and tools

    The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time.

  • Breadth Icon

    Variety of plate formats and cell types

    The imager is compatible with 6- to 384-well plates and enables use on suspension and adherent cell lines such as CHO, HEK, hybridomas, Per.C6®, Jurkat, SupT1, H-4-II-E, MCF-7, JT29, DLD1, and KB31.

  • Analysis Icon

    Intelligent analysis

    The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable.

  • Images Icon

    Clear, crisp images

    The imager features 4x objective lens with autofocus. High-resolution CCD camera images 1.85 microns per pixel, enabling accurate cell detection even at challenging areas such as well edges.

  • Automation Icon

    Custom automation options*

    The Advanced Workflow Engineering Solutions Team offer a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation.

*Price, time to deliver and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

Applications of CloneSelect Imager

  • Cell Line Development

    Cell Line Development for Recombinant Proteins

    Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.

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    Cell viability

    Cell viability

    Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.

  • Colony Forming Assay

    Colony Forming Assay

    The soft agar colony formation assay is a classical and renowned technique for characterizing the ability of cells to undergo anchorage-independent growth, a hallmark of carcinogenesis. After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically incubated with compounds that may affect colony growth. The CloneSelect Imager can image every well and automatically calculate confluence, estimate colony number and area, and track colony growth.

    Label-Free Cell Migration

    Label Free Cell Migration on CloneSelect Imager

    Wound healing assays are used in a range of disciplines to study cell migration, the coordinated movement of a cell population. A manual or standardized wound is generated on a monolayer of cells grown in microplates. The microplates can be screened against a library to study the effect on cell migration. The effect of a library of compounds or genes can be studied using this approach. The CloneSelect Imager enables the rapid imaging (<90 s per plate) and objective, automated analysis of cell migration. 

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  • Monoclonal Antibodies (mAbs)

    Monoclonal Antibodies (mAbs)

    Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.

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    Monoclonality

    Cell Line Development and Monoclonality Assurance

    Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.

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Specifications & Options of CloneSelect Imager

Resources of CloneSelect Imager

Presentations
Videos & Webinars
Stable Cell Line Development Generation

Stable Cell Line Development Workflow

Hybridoma Workflow

Hybridoma Workflow

Cell Line Development Workflows

The many and varied workflows of cell line development

Rapid identification of Neutralizing Antibodies

Optimized workflow for rapid identification of neutralizing antibodies against viral particles

CloneSelect Imager Video

CloneSelect Imager

Selection GPCR Cell Line

Identification and Selection of GPCR Cell Lines with ClonePix 2

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Merck Research Laboratory

    A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line… View more

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.

    Contributors: Shuangping Shi, Russ G.G. Condon, Liang Deng, Jason Saunders, Finn Hung, Yung-Shyeng Tsao, Zhong Liu  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Rapid Monoclonality Verification Methods to Boost Cell Line Development

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time… View more

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in white light and fluorescence channels. By using fluorescence to identify cells, monoclonality verification is easier and more conclusive than using white light imaging or microscopy alone.

    Contributors: Wilson Lew, Anna Forsyth, John Philips, Alison Glaser, Natalia Lysaya  
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  • Citation
    Dated: Jun 17, 2010
    Publication Name: BMC Biotechnology

    Accurate non-invasive image-based cytotoxicity assays for cultured cells

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid,… View more

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid, precise and simple method to detect living cells in mammalian cell cultures, using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and a microtiter plate reader.

    Contributors: Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk & Julian F Burke  
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