CloneSelect Imager (CSI and CSI FL)
High-speed fluorescence and white light imaging, intelligent data analysis, and monoclonality report generation
New for 2022, the CloneSelect Imager FL, a next-generation fluorescent imaging solution for the assurance of monoclonality and automated confluence across diverse cell types
Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Features
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White light and fluorescent imaging
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green).
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Data tools to accelerate research timelines
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing.
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Quickly image a variety of plate formats and cell types
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types.
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Intelligent analysis with easy-to-use software
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training.
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High-resolution images
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days.
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Custom automation options*
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.
Streamlined Workflow
Image. Analyze. Report.
(1) Image
Seed one cell per well and image at any point
- Optimize clonal outgrowth - The system is particularly useful for optimizing clonal outgrowth strategies when platform approaches are not suitable e.g. when investigating new cell lines or variants
- Diverse cell types - Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, IPSCs, and many other cell types.
(2) Analyze
Assess cell growth objectively and quantitatively
Track and view growth of every cell line
(2) Analyze
Growth curves calculated and displayed
Electronically track and store plate data: cell confluence, cell number estimation, and growth curve
(3) Report
Verify monoclonality
After initial seeding, CloneSelect Imager system can image every well, at any time point, using a ‘loci of growth’ functionality to highlight those wells that contain a single colony.
- Focus on wells with a single loci of growth and view image history to verify monoclonality with the CloneSelect Imager using white light
- With the CloneSelect Imager FL automatically verify monoclonality from day zero with fluorescense
(3) Report
Monoclonality Report
Provides image evidence of monoclonal cell bodies
With a few simple clicks, the Monoclonality Report feature on the CloneSelect™ Imager (CSI) objectively organizes the supporting image evidence needed to establish clonality into an easily shareable report, saving researchers hours typically required to do the same process manually.
- Easily identify and select single-cell and artifact regions to include in report
- Export high-resolution images of single cells, artifacts, and entire well (optional)
- Automatically identify single cells with fluorescence version of CloneSelect Imager
- Publish monoclonality report in PDF or Word Format
(3) Report
Monoclonality Report Feature
Automatically generate image-based data in shareable format
View entire well at final time point
Quickly determine clonality of a cell line by visually inspecting the presence of multiple colonies in a single well. For example, the well on the left shows one colony while the cell on the right shows two.
Monitor cell line development over time
To charachterize the growth from a single cell to a colony, cell regions can be designated and adjusted for each time point in a series. The image bellow shows a single cell on Day 0 and two cells on Day 1 confirm monoclonality.
(3) Report
Monoclonality Report Feature
Automatically generate image-based data in shareable format
Divide an entire well into individual images
Export an entire well into 81 separate images to objectively confirm the absence of another cell. Below, a selected region is displayed over time.
Highlight regions to review non-cell objects
Selectively highlight parts of a well to differentiate cells from ambiguous objects. A selected artifact region and its corresponding images over time is shown here.
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Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
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eBook
Tips from the cell line development front
Tips from the cell line development front
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Applications of CloneSelect Imager
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Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Cell viability
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.
Cell viability
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.
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Colony Forming Assay
The soft agar colony formation assay is a classical and renowned technique for characterizing the ability of cells to undergo anchorage-independent growth, a hallmark of carcinogenesis. After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically incubated with compounds that may affect colony growth. The CloneSelect Imager can image every well and automatically calculate confluence, estimate colony number and area, and track colony growth.
Colony Forming Assay
The soft agar colony formation assay is a classical and renowned technique for characterizing the ability of cells to undergo anchorage-independent growth, a hallmark of carcinogenesis. After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically incubated with compounds that may affect colony growth. The CloneSelect Imager can image every well and automatically calculate confluence, estimate colony number and area, and track colony growth.
Label-Free Cell Migration
Wound healing assays are used in a range of disciplines to study cell migration, the coordinated movement of a cell population. A manual or standardized wound is generated on a monolayer of cells grown in microplates. The microplates can be screened against a library to study the effect on cell migration. The effect of a library of compounds or genes can be studied using this approach. The CloneSelect Imager enables the rapid imaging (<90 s per plate) and objective, automated analysis of cell migration.
Label-Free Cell Migration
Wound healing assays are used in a range of disciplines to study cell migration, the coordinated movement of a cell population. A manual or standardized wound is generated on a monolayer of cells grown in microplates. The microplates can be screened against a library to study the effect on cell migration. The effect of a library of compounds or genes can be studied using this approach. The CloneSelect Imager enables the rapid imaging (<90 s per plate) and objective, automated analysis of cell migration.
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Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Specifications & Options of CloneSelect Imager
Automation compatibility
API suite available for robotic integration. Please contact us for details
Resources of CloneSelect Imager
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Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
-
eBook
Tips from the cell line development front
Tips from the cell line development front
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Brochure
CloneSelect Imager
CloneSelect Imager
CloneSelect Imager system produces objective, quantitative, and consistent results in less time to overcome the challenges associated with conventional cell confluence techniques.…
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Brochure
CloneSelect Imager Monoclonality Report Feature
CloneSelect Imager Monoclonality Report Feature
Providing image evidence of monoclonality in the cell line development process is not as simple as exporting an image of a single cell. For example, high-resolution images of the ent…
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Brochure
Accelerate Cell Line Development
Accelerate Cell Line Development
Explore PDF to learn how you can accelerate your cell line development and get to your high value hits faster.
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Blog
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
Monoclonal assurance is undeniably critical in cell line development for generating therapeutics, such as monoclonal antibodies or cell-based therapies. To utilize a cell line for…
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Blog
Accelerated Production of Monoclonal Antibodies for the Treatment of SARS-CoV-2
Accelerated Production of Monoclonal Antibodies for the Treatment of SARS-CoV-2
With solutions from Molecular Devices, scientists can fast-track the FDA approval process and accelerate monoclonal antibody discovery The timeline to identify and develop…
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Brochure
Clone screening solutions
Clone screening solutions
Advance your microbial and mammalian clone screening with proven, automated technologies
Application Note
Direct screening cytotoxic and antiproliferative activities of drug compounds on human leukemia U937 cell
Direct screening cytotoxic and antiproliferative activities of drug compounds on human leukemia U937 cell
A fast, reliable and cost-effective high-throughput screening method for antiproliferation and toxicology evaluation in the early stage of drug discovery is highly attractive. The…
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Blog
The evolution of CHO cells’ role in cell line development
The evolution of CHO cells’ role in cell line development
Since the first approval of recombinant insulin and human growth hormone in the early 1980s, a multitude of recombinant protein therapeutics have been approved by regulatory…
Flyer
High assurance monoclonality for cell line development
High assurance monoclonality for cell line development
Double confirmation of clonality. Image based evidence confirming single cell deposition event, and single cell in 96- and 384-well plate post sort
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Blog
Get to know our Field Applications Scientist: Dwayne Carter
Get to know our Field Applications Scientist: Dwayne Carter
Dwayne Carter gives us a taste of 3D bioprinting, clone screening, and Caribbean cuisine Dwayne Carter is a cell biologist and educator who joined Molecular Devices in November 2020…
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eBook
Accelerate vaccine development
Accelerate vaccine development
Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
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eBook
Optimize your high-value cell lines
Optimize your high-value cell lines
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
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Application Note
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
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Application Note
The diverse utility of CloneSelect Imager in label-free imaging applications
The diverse utility of CloneSelect Imager in label-free imaging applications
A variety of label-free imaging applications using the CloneSelect Imager, such as colony formation assays and clonality assessment, are presented.
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Application Note
Improved productivity of cell line development using fully integrated automated solutions: a series of case studies with the CloneSelect Imager
Improved productivity of cell line development using fully integrated automated solutions: a series of case studies with the CloneSelect Imager
Cell line development requires screening tens of thousands of clones in order to find the few clones that are stable, grow well, and produce high yields of bioproduct. In addition to…
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Application Note
Confident assurance of clonality using calcein AM with minimal effect on viability
Confident assurance of clonality using calcein AM with minimal effect on viability
The development of cell lines that express a specified protein of interest is critical to the generation of biopharmaceutical molecules such as monoclonal antibodies. To establish a…
Presentations
Developing High Throughput Mammalian Expression Platforms
Developing High Throughput Mammalian Expression Platforms
Download PDF and explore more about high throughput mammalian expression platforms & challenges with mammalian systems.
Scientific Poster
Accelerated one-step screening and selection of PER.C6® clones using ClonePix technology
Accelerated one-step screening and selection of PER.C6® clones using ClonePix technology
In this poster, we present data demonstrating that CloneMedia-PER.C6 supports the growth of cells into discrete colonies that can be imaged, ranked and picked using the ClonePix plat…
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Scientific Poster
Rapid High-Throughput Screening and Selection for Clonal Populations of Therapeutic Protein/Antibody Producer Cells and Reporter Cell-Lines, Using Fluorescence Cell Based Assay Techniques
Rapid High-Throughput Screening and Selection for Clonal Populations of Therapeutic Protein/Antibody Producer Cells and Reporter Cell-Lines, Using Fluorescence Cell Based Assay Techniques
This PDF file explores ClonePix FL Technology which is based on the culture of mammalian cell lines in semisolid media originating from one single parent cell.
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Scientific Poster
High Throughput Screening and Selection of Clonal Populations of Reporter Cell Lines, using Fluorescence Cell Based Assay Techniques
High Throughput Screening and Selection of Clonal Populations of Reporter Cell Lines, using Fluorescence Cell Based Assay Techniques
Advances in cell culture techniques are facilitating development of novel screening assays which enable drug discovery programmes to rely on cell-based assays for high-throughput scr…
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Application Note
Objective quantification of cell migration on the CloneSelect Imager System
Objective quantification of cell migration on the CloneSelect Imager System
Cell migration is a key process in normal development, immune function and wound healing. Abnormal cell migration is a feature of conditions such as cardiovascular disease and cancer.…
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Scientific Poster
Bypassing the Need for High Throughput Processing in Cell Line Development: Rapid, Selective in Situ Cell Screening by Phosphopeptide Recognition
Bypassing the Need for High Throughput Processing in Cell Line Development: Rapid, Selective in Situ Cell Screening by Phosphopeptide Recognition
Animal cell culture technology has been thrust to the forefront of the biopharmaceutical industry because of the superior fidelity of mammalian cells in producing clinically relevant…
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Brochure
Reagents and Supplies
Reagents and Supplies
Molecular Devices offer products and unrivaled solutions that utilize imaging & intelligent image analysis to support basic research, pharmaceutical & biotherapeutic developm…
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Scientific Poster
Rapid Selective in Situ Screening of Hybridomas in Semi-Solid Medium
Rapid Selective in Situ Screening of Hybridomas in Semi-Solid Medium
Explore a simple one-step hybridoma selective secretion assay for detecting and isolating antigen-specific, IgG-secreting hybridoma clones in as little as 8 days from fusion with min…
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Scientific Poster
Integration of Automated Colony Picking into Fast Track Cell Line Development Process
Integration of Automated Colony Picking into Fast Track Cell Line Development Process
Download this PDF to learn about fast track cell Line development process with integrated automated colony picker.
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Scientific Poster
Rapid Development of a Serum-Free, Suspension-Adapted CHO Cell Production Process
Rapid Development of a Serum-Free, Suspension-Adapted CHO Cell Production Process
Download PDF to explore the process for developing serum-free CHO cell line.
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Scientific Poster
Imaging, Screening and Automated Selection of Mouse Embryonic Stem Cells
Imaging, Screening and Automated Selection of Mouse Embryonic Stem Cells
Explore non-invasive method for mouse Embrionic Stem Cell culture colony number & growth rate monitoring and identification using specific probes.
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Scientific Poster
Non-Invasive, Label-Free Analysis of Cell Migration using CloneSelect™ Imager
Non-Invasive, Label-Free Analysis of Cell Migration using CloneSelect™ Imager
Explore CloneSelect Imager’s Migration that provides a simple, but quantitative & accurate measure of cell migration based on objective, sensitive label-free cell detection.
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Scientific Poster
Rapid Monoclonality Verification Methods to Boost Cell Line Development
Rapid Monoclonality Verification Methods to Boost Cell Line Development
Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA.
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Application Note
Confluence and growth curve generation on the CloneSelect Imager
Confluence and growth curve generation on the CloneSelect Imager
The ability to monitor cell proliferation has become increasingly important, particularly with the widespread use of in vitro cell lines as model systems. Proliferation rates can be…
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Application Note
Enhanced development of virus-specific hybridomas using ClonePix and CloneSelect Imager Technologies
Enhanced development of virus-specific hybridomas using ClonePix and CloneSelect Imager Technologies
The ClonePix System was employed in the high-throughput screening of hundreds of sub-cloned colonies from parental hybridoma material (historically parental line yields were less than…
Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow - AstraZeneca
Monoclonality Workflow
Stable Cell Line Development Workflow
Hybridoma Workflow
The many and varied workflows of cell line development
Optimized workflow for rapid identification of neutralizing antibodies against viral particles
CloneSelect Imager
Identification and Selection of GPCR Cell Lines with ClonePix 2
Number of Citations*: 74
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Dated: Jan 12, 2021Publication Name: Merck Research LaboratoryA High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line… View moreA high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.
Contributors: Shuangping Shi, Russ G.G. Condon, Liang Deng, Jason Saunders, Finn Hung, Yung-Shyeng Tsao, Zhong Liu
Go to article-
Dated: Jan 12, 2021Publication Name: Molecular Devices
Rapid Monoclonality Verification Methods to Boost Cell Line Development
Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time… View moreLimiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in white light and fluorescence channels. By using fluorescence to identify cells, monoclonality verification is easier and more conclusive than using white light imaging or microscopy alone.
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Dated: Jun 17, 2010Publication Name: BMC Biotechnology
Accurate non-invasive image-based cytotoxicity assays for cultured cells
High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid,… View moreHigh-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid, precise and simple method to detect living cells in mammalian cell cultures, using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and a microtiter plate reader.
Contributors: Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk & Julian F Burke
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Related Products & Services of CloneSelect Imager
Featured Applications
Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules,…
Cell viability
Cell line development requires the discovery of single cell-derived clones that produce high and…
Colony Forming Assay
The soft agar colony formation assay is a classical and renowned technique for characterizing the…
Label-Free Cell Migration
Wound healing assays are used in a range of disciplines to study cell migration, the coordinated…
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single…
Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of…
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