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New for 2022, the CloneSelect Imager FL, a next-generation fluorescent imaging solution for the assurance of monoclonality and automated confluence across diverse cell types

 

Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.

With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.

The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.

  • Validation Icon

    Demonstrated IND success

    The Monoclonality Report feature streamlines the creation of supporting documentation for regulatory agencies. Reports are automatically generated based on parameters you select. The Monoclonality Report is an audit-ready document that supports filing for an Investigational New Drug (IND) Application with the FDA. (21 CFR Part 312)

  • Accuracy Icon

    Multichannel imaging and automated confluence

    Algorithms are optimized for accurate cell detection and address varying cell types and conditions. Publication-ready high-resolution imaging provides automatic confluence analysis and monoclonality assurance.

  • Efficient Icon

    Rapid single cell confirmation

    The imager delivers industry leading acquisition times, imaging a 96-well plate in as little as two minutes and can verify monoclonality from day zero with fluorescence.

New for 2022 CloneSelect Imager FL

Features

  • Customizabl Icon

    White light and fluorescent imaging

    Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green).

  • Data Icon

    Data tools to accelerate research timelines

    The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing.

  • Breadth Icon

    Quickly image a variety of plate formats and cell types

    Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types.

  • Analysis Icon

    Intelligent analysis with easy-to-use software

    The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training. 

  • Images Icon

    High-resolution images

    High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days.

  • Automation Icon

    Custom automation options*

    The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.

*Price, time to deliver and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

Streamlined Workflow

Image. Analyze. Report.

 

(1) Image

Seed one cell per well and image at any point

  • Optimize clonal outgrowth - The system is particularly useful for optimizing clonal outgrowth strategies when platform approaches are not suitable e.g. when investigating new cell lines or variants
  • Diverse cell types - Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, IPSCs, and many other cell types.

CloneSelect Imager FL Optimize Clonal Outgrowth Workflow

Latest Resources

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Applications of CloneSelect Imager

  • Cell Line Development

    Cell Line Development for Recombinant Proteins

    Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.

    Download ebook 

    Cell viability

    Cell viability

    Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.

  • Colony Forming Assay

    Colony Forming Assay

    The soft agar colony formation assay is a classical and renowned technique for characterizing the ability of cells to undergo anchorage-independent growth, a hallmark of carcinogenesis. After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically incubated with compounds that may affect colony growth. The CloneSelect Imager can image every well and automatically calculate confluence, estimate colony number and area, and track colony growth.

    Label-Free Cell Migration

    Label Free Cell Migration on CloneSelect Imager

    Wound healing assays are used in a range of disciplines to study cell migration, the coordinated movement of a cell population. A manual or standardized wound is generated on a monolayer of cells grown in microplates. The microplates can be screened against a library to study the effect on cell migration. The effect of a library of compounds or genes can be studied using this approach. The CloneSelect Imager enables the rapid imaging (<90 s per plate) and objective, automated analysis of cell migration. 

    Read Application Note 

  • Monoclonal Antibodies (mAbs)

    Monoclonal Antibodies (mAbs)

    Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.

    Learn More 

    Monoclonality

    Cell Line Development and Monoclonality Assurance

    Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.

    Learn more 

Specifications & Options of CloneSelect Imager

 

Automation compatibility

API suite available for robotic integration. Please contact us for details

Resources of CloneSelect Imager

Presentations
Videos & Webinars
Monoclonality Assurance Using CloneSelect Single-Cell Printer

Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow - AstraZeneca

Monoclonality Workflow

Monoclonality Workflow

Stable Cell Line Development Generation

Stable Cell Line Development Workflow

Hybridoma Workflow

Hybridoma Workflow

The many and varied workflows of cell line development

The many and varied workflows of cell line development

Rapid identification of Neutralizing Antibodies

Optimized workflow for rapid identification of neutralizing antibodies against viral particles

CloneSelect Imager Video

CloneSelect Imager

Selection GPCR Cell Line

Identification and Selection of GPCR Cell Lines with ClonePix 2

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Merck Research Laboratory

    A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line… View more

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.

    Contributors: Shuangping Shi, Russ G.G. Condon, Liang Deng, Jason Saunders, Finn Hung, Yung-Shyeng Tsao, Zhong Liu  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Rapid Monoclonality Verification Methods to Boost Cell Line Development

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time… View more

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in white light and fluorescence channels. By using fluorescence to identify cells, monoclonality verification is easier and more conclusive than using white light imaging or microscopy alone.

    Contributors: Wilson Lew, Anna Forsyth, John Philips, Alison Glaser, Natalia Lysaya  
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  • Citation
    Dated: Jun 17, 2010
    Publication Name: BMC Biotechnology

    Accurate non-invasive image-based cytotoxicity assays for cultured cells

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid,… View more

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid, precise and simple method to detect living cells in mammalian cell cultures, using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and a microtiter plate reader.

    Contributors: Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk & Julian F Burke  
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