pH-induced Quaternary Assembly of Vitreoscilla Hemoglobin: the Monomer Exhibits Better Peroxidase Activity

Li W, et al., 1834(10), 2124-2132, Biochim Biophys Acta, 2013

In this work, the pH-dependent (pH 6.0-8.0) quaternary assembly of ferric Vitreoscilla hemoglobin (VHb) was investigated. The Octet RED96 system was used to characterize the self-association properties of VHb at various pH levels. VHb was biotinylated using NHS-PeG4-Biotin and loaded onto Streptavidin biosensors for the Octet experiments.

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