Identification of Malaria Parasite-Infected Red Blood Cell Surface Aptamers by Inertial Microfluidic SELEX (I-SELEX)

Birch C, et al., 1;5:11347, Sci Rep, 2015

Plasmodium falciparum (malaria parasite) enters the human red blood cells (RBCs) and traffic parasite-synthesized proteins to the RBC surface. These parasite-generated proteins present on the surface of infected RBCs tend to interact with various receptors on host cells and contribute to disease pathogenesis. This study introduces a new strategy called inertial microfluidic SELEX (I-SELEX) to identify high affinity aptamers that selectively recognize distinct surface protein targets present on parasite infected RBCs. Aptamer binding studies were carried out using a Pall ForteBio BLItz instrument equipped with thrombin-loaded Streptavidin (SA) biosensors. Those high affinity aptamer reagents will be useful for mapping malaria parasite-infected RBC surface proteome in detail.

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