Chemical Cross-linking of HIV-1 Env for Direct TLR7/8 Ligand Conjugation Compromises Recognition of Conserved Antigenic Determinants

Feng Y, et al., 446, 56-65, Virology, 2013

This study examines the effects of chemically cross-linking a synthetic TLR7/8 ligand to surface-exposed lysine residues on the external subunit of the HIV-1 envelope glycoprotein, gp120. Unmodified and cross-linker conjugated gp120 were used for kinetics analysis on the Octet RED system. Anti-Human IgG Fc biosensors were used to capture the mAbs VRC01, F105 or 447-52D, which then were incubated with the gp120 proteins.

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