SpectraMax Plus 384 Microplate Reader

Begin your brilliant projects quickly, and then go even faster, with rapid UV-Vis absorbance reads for a variety of sample formats

Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The SpectraMax® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on the same instrument. Monochromator wavelength selection saves you the time and trouble of ever having to change out filters. Typical applications for this powerful reader include DNA and RNA quantification, enzyme kinetic assays, and ELISA based assays. When your project is ready for high throughput, take advantage of StakMax® automated microplate handling compatibility for full robotic integration.


  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 190-1000 nm in 1 nm increments
  • Easy assay optimization: Read a variety of sample formats from cuvettes to 96- and 384-well microplates in endpoint, kinetic, and spectral scan modes
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy.
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Quickly quantify DNA, RNA, and protein samples as low as 2 µL with the SpectraDrop Micro-Volume Microplate
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker



Microplate Reader Validation


The SpectraMax Plus384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Micro volume applications
  • Microbial growth
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays


SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature

Softmax Pro Software Data Analysis

SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions    
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment's needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience      
  • Contemporary user community enabling protocol exchange and expertise
    sharing among our numerous users

Softmax Pro Software Data Analysis




* available in select instrument models

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13.6 kg)
Power Source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 190-1000nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth ≤ 2.0 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (Cuvette) < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Stray Light ≤ 0.07% at 230 nm
OD ≥ 3.15
Light Source Xenon Flash Lamp
Microplate Read Time - Endpoint 96-Well: 9 seconds
384-Well: 29 Seconds
Microplate Read Time - Kinetic 96-Well: 9 second interval
384-Well: 29 second internal
Microplate Read Time - Speed Read Mode 96-Well: 5 seconds
384-Well: 16 seconds
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate) ± 0.5 °C @ 37 °C


SpectraMax® Plus 384 Microplate Reader


Product Part Number
SpectraMax Plus 384 Microplate Reader PLUS 384
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Quartz 96-well microplate for UV measurements R8024
Quartz 96-well half-area microplate for UV measurements R8028
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Cuvette Validation Set 0200-2420
Microplate Reader Shelf 9000-0756

Number of Citations*: 8,230

Latest Citations:
For a complete list, please click here.

Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology

E Candeias, AI Duarte, I Sebastião… - Molecular …, 2016 - Springer
... Briefly, 10 μL of each brain cortical homogenate were added to 1 mL of Calimat
reagent and the absorbance read at 550 nm, 37 °C, in a SpectraMax Plus 384
multiplate reader (Molecular Devices, Wokingham, UK). Results ...

Turkish propolis supresses MCF-7 cell death induced by homocysteine

M Tartik, E Darendelioglu, G Aykutoglu… - Biomedicine & …, 2016 - Elsevier
... Later, WST-1 reagent (5 μl) was added to each well. After 4 h incubation, absorbance of each
well were measured at 450 nm (reference: 630 nm) by SpectraMax Plus 384 Microplate Reader
(Molecular Devices, USA). 2.4. Detection of lipid peroxidation. ...

PHLPP2 down regulation influences nuclear Nrf2 stability via Akt-1/Gsk3β/Fyn kinase axis in acetaminophen induced oxidative renal toxicity: Protection accorded by …

A Mathur, F Rizvi, P Kakkar - Food and Chemical Toxicology, 2016 - Elsevier
... bromide (MTT) at a final concentration of 5 mg/ml to the cells and incubated for 4 h. After removing
the supernatant media, 100% DMSO was added to dissolve formazan crystals and the absorbance
was measured at 530 nm on Spectramax plus 384 microplate based UV/visible ...

Phosphatase 2A Inhibition Affects Endoplasmic Reticulum and Mitochondria Homeostasis Via Cytoskeletal Alterations in Brain Endothelial Cells

AI PlácidoCMF Pereira, SC Correira, C Carvalho… - Molecular …, 2016 - Springer
... Mol Neurobiol Page 3. (SpectraMax Plus 384, Molecular Devices, San Francisco, CA, USA). ...
Caspase-like activi- ties were determined by measuring substrate cleavage at 405 nm in
a microplate reader (SpectraMax Plus 384, Molecular Devices). ...

Organophosphate Flame Retardants Act as Endocrine-Disrupting Chemicals in MA-10 Mouse Tumor Leydig Cells

G Schang, B Robaire, BF Hales - Toxicological Sciences, 2016 - Soc Toxicology
... Cellular viability assay. After treatment, cells were washed and incubated with MTT reagent for
4 h. Cells were lysed with 100 μL DMSO, and absorbance was read at 570 nM using a
SPECTRAmax Plus 384 (Molecular Devices, Sunnyvale, California). Cell counts. ...


Copper and hypoxia modulate transcriptional and mitochondrial functional-biochemical responses in warm acclimated rainbow trout (Oncorhynchus mykiss)

R Sappal, M Fast, S Purcell, N MacDonald… - Environmental …, 2016 - Elsevier
... gill mitochondria. The protein concentrations of liver and gill mitochondrial
suspensions were initially measured spectrophotometrically (Spectramax Plus 384,
Molecular Device, Sunnyvale, CA) by the Bradford (1976) method. ...


Aryl hydrocarbon receptor-dependent apoptotic cell death induced by the flavonoid chrysin in human colorectal cancer cells

SM Ronnekleiv-Kelly, M Nukaya, CJ Díaz-Díaz… - Cancer letters, 2016 - Elsevier
... Cell viability was measured using CellTiter-Fluor TM cell viability assay kit (Promega, Madison
WI). The fluorescence (excitation 390 nm, emission 460 nm) was detected using SpectraMax
Plus 384 microplate reader (Molecular Devices). Cytotoxicity and apoptosis assay. ...

Zinc supplementation leads to immune modulation and improved survival in a juvenile model of murine sepsis

HA Ganatra, BM Varisco, K Harmon, P Lahni… - Innate …, 2016 -
... Color change was measured on a SpectraMax Plus 384 Microplate Reader (Molecular Devices,
Sunnyvale, CA, USA) using 412 nm absorbance, and the colorimetric change was used to
calculate MPO concentration in each sample as per the manufacturer's instructions. ...

The plastidial starch phosphorylase from rice endosperm: catalytic properties at low temperature

SK Hwang, S Singh, B Cakir, H Satoh, TW Okita - Planta, 2016 - Springer
... Absorbance was measured at 650 nm using a SpectraMax Plus 384 Microplate Reader
(Molecular Devices). ... Absorbance was measured every 2 min at 348 nm using a
SpectraMax Plus 384 Microplate Reader (Molecular Devices). ...

[HTML] Safety profile of the intravenous administration of brain-targeted stable nucleic acid lipid particles

M Conceição, L Mendonça, C NóbregaC Gomes… - Data in brief, 2016 - Elsevier
... How data was acquired, Microscope (Zeiss PALM Axiovert 200M), Real-time PCR system (Step
One Plus Real-Time PCR System, Applied Biosystems), Spectrophotometer (Spectramax Plus
384, Molecular Devices). Results were analyzed using GraphPad Prism software. ...



* as of December 6, 2016. Source: Google Scholar.