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Filterless readers provide consistent results with steady temperature regulation

The SpectraMax ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. The temperature independent PathCheck sensor automatically normalizes and ensures accurate absorbance values by detecting pipetting errors. Combined with a wide application base, industry-leading SoftMax® Pro Software and validation tools, these readers fit in every lab and every budget.

  • Eliminate filters

    Eliminate filters

    Eliminate the need for purchasing and changing out filters with a monochromator-based wavelength selection for visible and UV-visible absorbance.

  • Get consistent results

    Get consistent results

    Mix samples thoroughly in microplate wells with user-programmable Automix feature and monitor temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C.

  • Integrate with ease

    Integrate with ease

    Measure micro-volumes: Compatible with the SpectraDrop™ Micro-Volume Microplate, enabling high-throughput measurement of low volumes (<5um) – ideal for DNA, RNA and protein quantification.

SpectraMax ABS Plus Feature Demonstration

Features

  • Measure Icon

    Pipetting errors eliminated

    This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. It also allows for the elimination of standard curves. 

  • Optimized Icon

    Optimized optical path

    The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. The spectral resolution ensures accuracy of DNA absorbance measurements.

  • Optics Icon

    Eight-channel optics

    The optical design of the reader enables simultaneous detection of 8 wells. The eight-channel system delivers both precision and speed across the microplate.

  • Performance Icon

    Dual wavelength reads

    The reader delivers high performance with round-bottom, flat-bottom or half-area well plates. Dual wavelength readings report the actual absorbance at each wavelength making data analysis convenient.

  • Flexibility Icon

    Flexibility to read a variety of plate formats

    SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate.

  • Validation Icon

    Validation made easy

    Verify the accuracy of your absorbance measurements with the SpectraTest® ABS1 Absorbance Validation Plate, using NIST-traceable standards. Testing can be done in your own lab, on your own schedule.

Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational specifications
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs

 

Applications of SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers

Specifications & Options of SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers

 

*Using lowest settings and speed read when available.

**Only available on SpectraMax ABS Plus.

Resources of SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers

Presentations
Videos & Webinars
Microplate Technologies for Food and Beverage Testing

Microplate analysis technologies for food and beverage quality testing

Week 5 Video Thumbnail

Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?

Week 4 Video Thumbnail

Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy

Week 3 Video Thumbnail

Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"

 Week 1 Video Thumbnail

Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!

Week 2 Video thumbnail

Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!

ELISA endpoint protocol

How to configure an ELISA endpoint protocol

SpectraMax ABS and ABS Plus Absorbance Microplate Readers

SpectraMax ABS and ABS Plus Absorbance Microplate Readers

SpectraMax ABS Plus Feature Demonstration

  • Citation
    Dated: Nov 01, 2016
    Publication Name: Southern California Conferences for Undergraduate Research

    Effect of Caffeine on Biofilm Formation and Capsule Size on Pseudomonas aeruginosa

    Caffeine is a stimulant naturally found in coffee beans and is consumed daily by millions of people. Pseudomonas aeruginosa (PA) is a gram-negative opportunistic microbe known to be the main cause for pneumonia in cystic fibrosis and is observed in many hospital-acquired infections. View more

    Caffeine is a stimulant naturally found in coffee beans and is consumed daily by millions of people. Pseudomonas aeruginosa (PA) is a gram-negative opportunistic microbe known to be the main cause for pneumonia in cystic fibrosis and is observed in many hospital-acquired infections.

    Contributors: Byron Trinh, California State Polytechnic University - Pomona, Cynde Mai, California State Polytechnic University - Pomona, John K. Chan Dr., California State Polytechnic University - Pomona  
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  • Citation
    Dated: Nov 11, 2010
    Publication Name: Journal of Dairy Science

    Feeding rolled barley grain steeped in lactic acid modulated energy status and innate immunity in dairy cows

    Feeding dairy cows large proportions of cereal grain is commonly associated with rumen acidosis, activation of innate immunity, and perturbation of intermediary metabolism. We previously showed that steeping barley grain in 0.5% lactic acid (LA) decreased the rate of starch degradation, lowered the risk of subacute rumen acidosis, modulated rumen… View more

    Feeding dairy cows large proportions of cereal grain is commonly associated with rumen acidosis, activation of innate immunity, and perturbation of intermediary metabolism. We previously showed that steeping barley grain in 0.5% lactic acid (LA) decreased the rate of starch degradation, lowered the risk of subacute rumen acidosis, modulated rumen fermentation profile, and increased milk fat content in dairy cows. This study sought to investigate whether feeding of LA-treated barley grain would affect carbohydrate and lipid metabolism as well as innate immunity. Eight rumen-fistulated late-lactation (approximately 217 d in milk, DIM) Holstein cows were randomly assigned, in a 2 × 2 crossover design, to 1 of the 2 dietary treatments consisting of 27% (dry matter basis) rolled barley grain steeped for 48 h in an equal volume (wt/vol) of tap water (CTR) or 0.5% LA (TRT). Each experimental period lasted 21 d, with the first 11 d for diet adaptation. Blood and rumen samples were collected on d 12, 15, 17, and 21 of the experimental period before the morning feeding to evaluate the effects of dietary treatment on preprandial day-to-day variation of plasma and rumen variables. To establish the effect of treatment on diurnal variation of plasma variables, blood samples were collected on the last day of each period at 0, 2, 4, 6, 8, 10, and 12 h after the morning feeding (i.e., 0800 h). Results of the day-to-day study showed that cows fed the TRT diet had greater overall preprandial concentrations of glucose, cholesterol, and insulin, and a lower concentration of haptoglobin in plasma. Diurnal data indicated lower concentrations of haptoglobin and serum amyloid A and a tendency for greater plasma lactate in cows fed the TRT diet. A treatment by time interaction was observed for glucose, lactate, insulin, haptoglobin, and lipopolysaccharide-binding protein, suggesting a role for both the processing of grain and the time of sampling on those variables. No effect of diet on plasma concentrations of cortisol, β-hydroxybutyrate, and nonesterified fatty acids or rumen endotoxin was evidenced. Taken together, our results demonstrated that feeding barley grain steeped in 0.5% LA modulated both energy status and innate immunity of dairy cows fed relatively high levels (45% of dry matter) of dietary concentrate.

    Contributors: S. Iqbal, Q Zebeli , A.Mazzolari. S.M. Dunn, B.N. Ametaj  
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  • Citation
    Dated: Feb 02, 2008
    Publication Name: Journal of Dairy Science

    Feeding High Proportions of Barley Grain Stimulates an Inflammatory Response in Dairy Cows

    The objective of this study was to evaluate effects of feeding increasing proportions of barley grain on acute phase response in lactating dairy cows. Eight cannulated primiparous (60 to 140 d in milk) Holstein dairy cows were assigned to 4 diets in a 4 × 4 Latin square experimental design. The experimental period lasted for 21 d, with 11 d of… View more

    The objective of this study was to evaluate effects of feeding increasing proportions of barley grain on acute phase response in lactating dairy cows. Eight cannulated primiparous (60 to 140 d in milk) Holstein dairy cows were assigned to 4 diets in a 4 × 4 Latin square experimental design. The experimental period lasted for 21 d, with 11 d of adaptation and 10 d of measurements. Cows were fed the following diets: 1) no barley grain in the diet, 2) 15% barley grain, 3) 30% barley grain, and 4) 45% barley grain, as well as barley and alfalfa silage and alfalfa hay at 85, 70, 55, and 40% [dry matter (DM) basis]. All cows were supplemented with a 15% concentrate mix. Blood and rumen fluid samples were collected on d 1, 3, 5, 7, and 10 of the measurement period, and pH and endotoxin content were measured in rumen samples. Concentrations of serum amyloid A, lipopolysaccharide-binding protein, haptoglobin, and C-reactive protein in plasma were measured by ELISA. Feeding high proportions of barley grain at 0, 15, 30, and 45% of DM was associated with lower feed intake (32.6, 32.9, 27.34, and 25.18 kg/d ± 1.30, respectively), lower ruminal pH (6.8, 6.7, 6.7, and 6.5 ± 0.03, respectively), and higher DM intake (13.33, 15.28, 14.68, and 16.04 ± 0.63 kg/d, respectively) and milk production (27.2, 28.2, 29.0, and 31.0 ± 1.2 kg/d, respectively). Ruminal endotoxin increased in cows receiving 30 and 45% barley grain (5,021, and 8,870 ± 393 ng/mL, respectively) compared with those fed no grain or 15% barley grain (654 and 790 ± 393 ng/mL, respectively). Plasma concentrations of serum amyloid A, lipopolysaccharide-binding protein, and C-reactive protein increased in cows given higher (30 and 45%) proportions of grain. Plasma haptoglobin was not affected by treatments. In conclusion, feeding dairy cows high proportions (30 and 45% DM basis) of barley grain was associated with lower feed intake and rumen pH, increased endotoxin in the rumen fluid, and stimulation of an inflammatory response.

    Contributors: D.G.V. Emmanuel, S.M.Dunn, B.N.Ametaj  
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Absorbance Microplate Readers

 

SpectraMax® ABS Plus Microplate Reader

Product

Part Number

SpectraMax ABS Plus Microplate Reader with SoftMax Pro Software ABS PLUS
SpectraTest ABS2 Absorbance Validation Plate 0200-6191
StakMax Microplate Handling Stacker StakMax
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Cuvette Validation Set 0200-2420

 

SpectraMax® ABS Microplate Reader

Product Part Number
SpectraMax ABS Microplate Reader with SoftMax Pro Software        ABS
SpectraTest ABS2 Absorbance Validation Plate 0200-6191
StakMax Microplate Handling Stacker StakMax
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262

 

SpectraMax® 340PC384 Microplate Reader

Product

Part Number

340PC384 Microplate Reader 340PC 384
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756

 

SpectraMax® Plus 384 Microplate Reader

Product Part Number
SpectraMax Plus 384 Microplate Reader PLUS 384
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Cuvette Validation Set 0200-2420
Microplate Reader Shelf 9000-0756

 

 SpectraMax® 190 Microplate Reader

Product

Product Number

SpectraMax 190 Microplate Reader with SoftMax Pro Software 190
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756

 

VersaMax ELISA Microplate Reader

Product

Part Number

VersaMax ELISA Microplate Reader VERSAMAX
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756

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