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SoftMax Pro Software
The most published microplate reader control and data analysis software
Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis
SoftMax® Pro Software for Windows 10 is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. Compliance tools are available for regulated laboratories providing end-to-end chain of custody.
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Simplify data measurement
The software offers a variety of options to initiate the measurement of a microplate assay through prewritten protocols with included acquisition settings or by setting custom experiment parameters.
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Import and analyze complex data
Import raw data in microplate format acquired from any scientific instrument, consolidate data reduction from diverse data sources, and reduce errors with consistent data evaluation methods.
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Publish like a pro
The software includes tools to publish reports with flexible data output formats. Data can be viewed in a lab notebook layout, saved as a PDF file, exported to an Excel file or to XML for LIMS integration.

SoftMax Pro 7 Software
Features
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Built-in protocols
Use over 160 protocols with preconfigured assay parameters for a wide variety of common assays including protocols for our entire suite of reagents, SpectraTest® Validation Plates, and adapter plates.
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Advanced reader control
Build custom assay workflow, run multitask kinetics, and pause and resume kinetic reads with the discontinuous kinetics feature.
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Flexible calculations
Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations of relative potency, EC50, and Z factors.
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Project management made easy
Group multiple plate reads in one protocol. Display results, conditional pass/fail statements, mini graphs, and images in the notes section for easy viewing of overall project results.
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Secure electronic record keeping
Controlled user access is provided through a granular permission structure and unique logins with electronic signature support for verification, authorization, and approval, including audit trails.
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Validation tools reduce cost
Extensive suite of microplate reader validation tools can reduce the cost and time of validation by 50% when compared to using multiple platforms to collect and analyze data.
Proven GxP solutions to assure data integrity and compliance
Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.
- Best-in-class microplate readers and washers support all your assay needs
- IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
- Software installation services verify and document that required components are installed to operational specifications
- Software validation service supports FDA 21 CFR Part 11 guidelines
- Validation plates test the performance of your microplate reader using traceable materials for reliable results

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Application Note
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about 250…
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eBook
Software compliance for GMP/GLP labs
Software compliance for GMP/GLP labs
Become 21 CFR Part 11 compliant with SoftMax Pro 7.1.1 GxP Software.
Applications of SoftMax Pro Software
Specifications & Options of SoftMax Pro Software
Helpful Tips:
- To prevent data loss, turn off all sleep and hibernation settings for the hard disk, the CPU, and the USB ports
- Disable automatic Windows Updates
- Update WIndows manually when the instrument isn't being used by the software; these options can be enabled in Windows Control Panel
Note: Installing and using the SoftMax Pro Software on the Windows XP operating system is no longer supported. The software is neither tested nor validated on Windows XP.
Resources of SoftMax Pro Software
Application Note
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about…
eBook
Software compliance for GMP/GLP labs
Software compliance for GMP/GLP labs
Become 21 CFR Part 11 compliant with SoftMax Pro 7.1.1 GxP Software.
Flyer
SoftMax Pro Software Import and Export Tools
SoftMax Pro Software Import and Export Tools
The import and export functionality of SoftMax® Pro Software allows for importing plate templates as xls files which are saved in a .txt format. Plate templates can be exported in a…
Infographic
History of ELISA
History of ELISA
ELISA (enzyme-linked immunosorbent assay) is a method used to quantitatively detect an antigen within a sample. An antigen is a toxin or other foreign substance, for example a flu vi…
eBook
From ELISA to epigenetics with HTRF
From ELISA to epigenetics with HTRF
HTRF® is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Assays encompass the areas of G protein-coupled receptors (GPCR), kinases and cel…
Application Note
Importing sample information into SoftMax Pro Software using template tools
Importing sample information into SoftMax Pro Software using template tools
SoftMax® Pro 6 or 7 Software enables the entry of some sample information via the template editor.
Application Note
Advanced kinetic analysis of a bacterial growth assay
Advanced kinetic analysis of a bacterial growth assay
Difficult-to-kill bacteria have become a problem in hospital-acquired infections. Identifying compounds which kill these bacteria is of interest to many pharmaceutical companies.…
Data Sheet
SpectraTest Validation Packages
SpectraTest Validation Packages
Microplate readers from Molecular Devices are designed to provide consistent performance for many years. In keeping with best practices, reader performance should still be validated…
Application Note
Parallel line analysis and relative potency in SoftMax Pro 7 Software
Parallel line analysis and relative potency in SoftMax Pro 7 Software
Biological assays are frequently analyzed with the help of parallel line analysis (PLA). PLA is commonly used to compare dose-response curves where there is no direct measurement of a…
Application Note
Complex Data Analysis With SoftMax Pro 6.4.1 Import Feature
Complex Data Analysis With SoftMax Pro 6.4.1 Import Feature
Accurate analysis of data is critical to ensuring integrity of experimental results. Consistent data evaluation methods reduce the opportunity for mis-analysis and process-related…
Application Note
Selecting the best weighting factor in SoftMax Pro 7 Software
Selecting the best weighting factor in SoftMax Pro 7 Software
Choosing the proper weighting factor is crucial to obtaining the best curve fit and therefore, obtaining the curve fit parameter values that make the curve fit model as close as…
Application Note
Selecting the best curve fit in SoftMax Pro 7 Software
Selecting the best curve fit in SoftMax Pro 7 Software
Choosing the correct curve fit model is crucial when determining important characteristics of data such as the rate of change, upper and lower asymptotes of the curve, or the EC50/IC50…
Application Note
Analyze cardiomyocyte spheroid contractions with SoftMax Pro Software
Analyze cardiomyocyte spheroid contractions with SoftMax Pro Software
Cell-based models for compound screening have become more complex in order to represent complexity of biological systems. Live-cell imaging and threedimensional models provide valuable…
Application Note
Multitask kinetic measurements of IPTG’s effects on protein expression and cell growth
Multitask kinetic measurements of IPTG’s effects on protein expression and cell growth
Measuring several different signal outputs simultaneously over time is especially useful for studying a protein or compound’s effect on cellular growth or gene expression. In this…


Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?

Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy

Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"

Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!

Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!

Using the export function in SoftMax Pro 7 Software

SoftMax Pro 6.4.1 Import Feature

SoftMax Pro 6.5 Acquisition View

Creating a Multitask Kinetics Workflow with the SoftMax Pro 7 Workflow Editor

SoftMax Pro 7 Software

Creating a New Graph in SoftMax Pro 7 Software

Modifying Existing Graphs in SoftMax Pro 7 Software

Setting up a Plate Template in SoftMax Pro 7 Software

Using the Autosave Option in SoftMax Pro 7 Software

Setting up an Absorbance Assay in SoftMax Pro 7 Software

Curve Fitting Features and PLA: A Practical Guide for SoftMax Pro 6

SoftMax Pro 6 Software

How to Do Microplate Data Acquisition and Data Analysis Using SoftMax Pro 6 Software

Getting Started with SoftMax Pro 6 Software

SoftMax Pro 6 Data Acquisition Tips and Tricks

SoftMax Pro 6 Data Acquisition Tips and Tricks (French)

SoftMax Pro 6 Data Acquisition Tips and Tricks (German)

SoftMax Pro 6.2 GxP: Compliance Without Complications

Setting up a Plate Template with Dilution in SoftMax Pro 7 Software
Number of Citations*: 13,800
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
- Dated: Dec 15, 2020Publication Name: Vaccine
Development and characterization of a standardized ELISA including a reference serum on each plate to detect antibodies induced by experimental malaria vaccines
Enzyme linked immunosorbent assay (ELISA) has been widely used to measure antibody titers for evaluating the immunogenicity of a vaccine. However, there is as yet no generally accepted way of expressing the ELISA results in the case of experimental vaccines, since there is usually no uniform standard. Both end point and single dilution methods… View moreEnzyme linked immunosorbent assay (ELISA) has been widely used to measure antibody titers for evaluating the immunogenicity of a vaccine. However, there is as yet no generally accepted way of expressing the ELISA results in the case of experimental vaccines, since there is usually no uniform standard. Both end point and single dilution methods have significant disadvantages. In this paper, we obtained reproducible data with fewer dilutions of samples by addition of serially diluted standard serum to each ELISA plate. Since this ELISA method gives reliable antibody titer with less labor than other methods, it can strongly support vaccine development.
Contributors: Kazutoyo Miura, Andrew C. Orcutt, Olga V.Muratova, Louis H.Miller Allan, Saul, Carole A. Long
Go to article - Dated: Oct 01, 2018Publication Name: Crop Protection
Identification and characterization of abamectin resistance in Tetranychus urticae Koch populations from greenhouses in Turkey
The two-spotted spider mite, Tetranychus urticae Koch is one of the most serious pests in greenhouses and has developed high resistance to many classes of acaricides rapidly. Three T. urticae populations were collected from vegetable greenhouses in Antalya and Muğla, Turkey. These populations showed high resistance levels to abamectin ranging… View moreThe two-spotted spider mite, Tetranychus urticae Koch is one of the most serious pests in greenhouses and has developed high resistance to many classes of acaricides rapidly. Three T. urticae populations were collected from vegetable greenhouses in Antalya and Muğla, Turkey. These populations showed high resistance levels to abamectin ranging between 223 and 404 fold compared to a susceptible population. The interaction of some synergists (piperonyl butoxide; PBO, diethyl maleate; DEM and S-benzyl O,O-diisopropyl phosphorothioate; IBP) with abamectin was analyzed showing possible implication of esterases in resistances in the three populations studied. The activities of esterase, glutathione S-transferase (GST) and cytochrome P450 (p450) was determined using α-naphthyl acetate, 1-chloro-2,4 dinitrobenzene (CDNB) and 7-ethoxycoumarin (7-EC) as substrates, respectively. In all field populations, esterase, glutathione S-transferase and P450 activities were higher, when compared to the susceptible population (GSS). The presence of known abamectin resistance target site mutations (G314D and G326E) on the glutamate gated chloride channels was also examined. However, no target site–resistance mutation was detected in all three populations. According to our results, detoxification enzymes, but no target site intensivity seem to play role in abamectin resistance in field T. urticae populations from Turkey.
- Dated: Apr 04, 2001Publication Name: Journal of Investigative Dermatology
An Alternative Approach to Depigmentation by Soybean Extracts via Inhibition of the PAR-2 Pathway
The protease-activated receptor 2, expressed on keratinocytes but not on melanocytes, has been ascribed functional importance in the regulation of pigmentation by phagocytosis of melanosomes. Inhibition of protease-activated receptor 2 activation by synthetic serine protease inhibitors requires keratinocyte-melanocyte contact and results in… View moreThe protease-activated receptor 2, expressed on keratinocytes but not on melanocytes, has been ascribed functional importance in the regulation of pigmentation by phagocytosis of melanosomes. Inhibition of protease-activated receptor 2 activation by synthetic serine protease inhibitors requires keratinocyte-melanocyte contact and results in depigmentation of the dark skinned Yucatan swine, suggesting a new class of depigmenting mechanism and agents. We therefore examined natural agents that could exert their effect via the protease-activated receptor 2 pathway. Here we show that soymilk and the soybean-derived serine protease inhibitors soybean trypsin inhibitor and Bowman-Birk inhibitor inhibit protease-activated receptor 2 cleavage, affect cytoskeletal and cell surface organization, and reduce keratinocyte phagocytosis. The depigmenting activity of these agents and their capability to prevent ultraviolet-induced pigmentation are demonstrated both in vitro and in vivo. These results imply that inhibition of the protease-activated receptor 2 pathway by soymilk may be used as a natural alternative to skin lightening.
Contributors: Christine Paine, Elizabeth Sharlow, Frank Liebel, Magdalena Eisinger, StanleyShapiro, MiriSeiberg
Go to article
SoftMax Pro 7 Software
Product |
Annual Subscription License |
Non-Expiring License |
SoftMax Pro Standard SoftwareWindows 10 compatible |
One year subscription to the latest version of SoftMax Pro 7. Includes one activation key, which expires after one year. Annual renewal. Part number:
Special pricing for verified academic institutions. For contact numbers outside the US, please visit our contact page. |
Non-expiring license for the latest version of SoftMax Pro 7. Includes four activation keys.
Part number:
Special pricing for verified academic institutions. For contact numbers outside the US, please visit our contact page. |
SoftMax Pro Importer XLSRequires SoftMax Pro 6.4.1 or higher |
One year subscription to enable import of data into SoftMax Pro from Excel based template. Includes one activation key, which expires after one year. Annual renewal. Part number: (Click below to purchase/download) |
|
SoftMax Pro Importer XLS and XMLRequires SoftMax Pro 6.4.1 or higher |
One year subscription to enable import of data into SoftMax Pro from Excel based template or XML file. Includes one activation key, which expires after one year. Annual renewal. Part number: (Click below to purchase/download) |
Non-expiring license to enable import of data into SoftMax Pro from Excel-based template or XML file. Includes one activation key for one computer. Part number: (Click below to purchase/download) SMP.IMPORT.XLS.AND.XML.NONEXP |
Related Products & Services of SoftMax Pro Software
Customer Breakthrough


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National Horizons Centre uses the SpectraMax iD5 & SoftMax Pro Software to train the next generation of scientists
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Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?