SoftMax Pro Software
The most published microplate reader control and data analysis software
The most published microplate reader control and data analysis software
SoftMax® Pro Software for Windows 10 is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. Compliance tools are available for regulated laboratories providing end-to-end chain of custody.
Use over 160 protocols with preconfigured assay parameters for a wide variety of common assays including protocols for our entire suite of reagents, SpectraTest® Validation Plates, and adapter plates.
Build custom assay workflow, run multitask kinetics, and pause and resume kinetic reads with the discontinuous kinetics feature.
Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations of relative potency, EC50, and Z factors.
Group multiple plate reads in one protocol. Display results, conditional pass/fail statements, mini graphs, and images in the notes section for easy viewing of overall project results.
Controlled user access is provided through a granular permission structure and unique logins with electronic signature support for verification, authorization, and approval, including audit trails.
Extensive suite of microplate reader validation tools can reduce the cost and time of validation by 50% when compared to using multiple platforms to collect and analyze data.
Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.
Helpful Tips:
Note: Installing and using the SoftMax Pro Software on the Windows XP operating system is no longer supported. The software is neither tested nor validated on Windows XP.
Application Note
Accurate quantification of nucleic acid concentration is important for downstream applications including transfection, cloning, PCR, and next generation sequencing (NGS). Often, these…
Application Note
Many microplate readers and spectrophotometers use a photomultiplier tube (PMT) to detect fluorescence and luminescence signals. PMTs work by initially detecting photons from the…
Blog
Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
Application Note
Monitoring for contaminants is a critical step during the production process in the pharmaceutical and medical device industries. A frequent contaminant, endotoxin, can cause fever,…
Application Note
Nucleic acid quantitation is an essential assay that is part of many workflows in genetics, molecular, and cellular biology. Various methods have been developed to quantitate nucleic…
Application Note
Firefly luciferase is a widely used reporter to study gene regulation and function. It is a very sensitive reporter due to the lack of any endogenous luciferase activity in mammalian…
Blog
SLAS2022, the Society for Lab Automation and Screening conference offered another exciting year for learning about innovative laboratory technologies. Whether you attended in-person…
eBook
Evaluating microplate readers doesn’t have to be overwhelming. First, consider your application needs. If your budget is modest, a single-mode reader dedicated to your main applicati…
Application Note
The MAPK/ERK cell signaling pathway begins with the activation of a receptor tyrosine kinase located at the cell surface, involves a series of kinase phosphorylation steps (kinase…
Application Note
Rapid, accurate, and frequent molecular testing is of critical importance in navigating the SARS-CoV-2 (COVID-19) pandemic.
Publications
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translational research. We describe here a novel…
Blog
The regulations for food and drug in the United States, described in the Title 21 of the Code of Federal Regulations, and the EudraLex Annex 11 in EU, are critical in ensuring safe…
Flyer
Get remote PhD level technical support from the experts that know our microplate readers and SoftMax® Pro GxP Software best. Only Molecular Devices SMPCare provides one-stop support…
Blog
Cheryl Bell talks about making the transition from manual microscopy to automated microscopy Our Field Applications Scientist, Cheryl Bell, was recently featured in a Women in…
Customer Breakthrough
A UK centre of excellence for the bioscience industry
Opened in Spring 2019, the National Horizons Centre (NHC) is an exciting £22M state-of-the art, purpose-…
Blog
For over 40 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Application Note
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about…
Publications
Scientists take many roads to overcoming bottlenecks in workflows. For example, a workstation including a microplate reader can handle multiple steps, which improves efficiency and a…
Infographic
ELISA (enzyme-linked immunosorbent assay) is a method used to quantitatively detect an antigen within a sample. An antigen is a toxin or other foreign substance, for example a flu vi…
eBook
HTRF® is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Assays encompass the areas of G protein-coupled receptors (GPCR), kinases and cel…
Customer Breakthrough
Based in France, ETAP-Lab is a Contract Research Organization (CRO) providing its clients with preclinical services in the fields of Dermatolo…
Application Note
SoftMax® Pro 6 or 7 Software enables the entry of some sample information via the template editor.
Brochure
SoftMax® Pro 7.1.2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Every step is…
Application Note
Difficult-to-kill bacteria have become a problem in hospital-acquired infections. Identifying compounds which kill these bacteria is of interest to many pharmaceutical companies.…
Flyer
The import and export functionality of SoftMax® Pro Software allows for importing plate templates as xls files which are saved in a .txt format. Plate templates can be exported in a…
Customer Breakthrough
Dr. Luis Gimenez-Lirola’s Lab, at Iowa State University in the College of Veterinary Medicine is developing assays for automated screening, and analysis of neutralizing antibody assa…
Data Sheet
Microplate readers from Molecular Devices are designed to provide consistent performance for many years. In keeping with best practices, reader performance should still be validated…
Application Note
Biological assays are frequently analyzed with the help of parallel line analysis (PLA). PLA is commonly used to compare dose-response curves where there is no direct measurement of a…
Application Note
Accurate analysis of data is critical to ensuring integrity of experimental results. Consistent data evaluation methods reduce the opportunity for mis-analysis and process-related…
Application Note
Choosing the proper weighting factor is crucial to obtaining the best curve fit and therefore, obtaining the curve fit parameter values that make the curve fit model as close as…
Application Note
Choosing the correct curve fit model is crucial when determining important characteristics of data such as the rate of change, upper and lower asymptotes of the curve, or the EC50/IC50…
Application Note
Cell-based models for compound screening have become more complex in order to represent complexity of biological systems. Live-cell imaging and threedimensional models provide valuable…
Application Note
Measuring several different signal outputs simultaneously over time is especially useful for studying a protein or compound’s effect on cellular growth or gene expression. In this…
Small Lab, Big Challenges - How the Right Reader can Impact Productivity
Critical Considerations for Choosing the Right Microplate Reader
Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?
Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy
Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"
Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!
Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!
Using the export function in SoftMax Pro 7 Software
SoftMax Pro 6.4.1 Import Feature
SoftMax Pro 6.5 Acquisition View
Creating a Multitask Kinetics Workflow with the SoftMax Pro 7 Workflow Editor
SoftMax Pro 7 Software
Creating a New Graph in SoftMax Pro 7 Software
Modifying Existing Graphs in SoftMax Pro 7 Software
Setting up a Plate Template in SoftMax Pro 7 Software
Using the Autosave Option in SoftMax Pro 7 Software
Setting up an Absorbance Assay in SoftMax Pro 7 Software
Curve Fitting Features and PLA: A Practical Guide for SoftMax Pro 6
SoftMax Pro 6 Software
How to Do Microplate Data Acquisition and Data Analysis Using SoftMax Pro 6 Software
SoftMax Pro 6 Data Acquisition Tips and Tricks
SoftMax Pro 6 Data Acquisition Tips and Tricks (French)
SoftMax Pro 6 Data Acquisition Tips and Tricks (German)
SoftMax Pro 6.2 GxP: Compliance Without Complications
Setting up a Plate Template with Dilution in SoftMax Pro 7 Software
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Virus neutralization assays measure neutralizing antibodies in serum and plasma, and the plaque reduction neutralization test (PRNT) is considered the gold standard for measuring levels of these antibodies for many viral diseases. We have developed procedures for the standard PRNT, microneutralization assay (MNA) and pseudotyped virus neutralization assay (PNA) for severe acute respiratory syndrome coronavirus 2. The MNA offers advantages over the PRNT by reducing assay time, allowing increased throughput and reducing operator workload while remaining dependent upon the use of wild-type virus. This ensures that all severe acute respiratory syndrome coronavirus 2 antigens are present, but Biosafety Level 3 facilities are required. In addition to the advantages of MNA, PNA can be performed with lower biocontainment (Biosafety Level 2 facilities) and allows for further increases in throughput. For each new vaccine, it is critical to ensure good correlation of the neutralizing activity measured using PNA against the PRNT or MNA. These assays have been used in the development and licensure of the ChAdOx1 nCoV-19 (AstraZeneca; Oxford University) and Ad26.COV2.S (Janssen) coronavirus disease 2019 vaccines and are critical for demonstrating bioequivalence of future vaccines.
Enzyme linked immunosorbent assay (ELISA) has been widely used to measure antibody titers for evaluating the immunogenicity of a vaccine. However, there is as yet no generally accepted way of expressing the ELISA results in the case of experimental vaccines, since there is usually no uniform standard. Both end point and single dilution methods have significant disadvantages. In this paper, we obtained reproducible data with fewer dilutions of samples by addition of serially diluted standard serum to each ELISA plate. Since this ELISA method gives reliable antibody titer with less labor than other methods, it can strongly support vaccine development.
Neutralizing anti-drug antibody (NAb) assays often have lower drug tolerance (DT) than trough drug concentrations, potentially under-estimating NAb incidence. To improve DT, drug-specific proteins were coupled to magnetic beads to deplete drug in the sample.
Hereditary angioedema (HAE) types I and II are characterized by functional C1 inhibitor (fC1-INH) deficiency which results in bradykinin overproduction. Sensitive, specific and robust methods to quantitate fC1-INH in human samples are required for diagnosing HAE and/or to measure pharmacodynamic activity of C1-INH drugs in clinical studies.
The widespread environmental contaminant β-methylamino-L-alanine (BMAA) is a developmental neurotoxicant that can induce long-term learning and memory deficits. Studies have shown high transplacental transfer of 3H-BMAA and a significant uptake in fetal brain.
The two-spotted spider mite, Tetranychus urticae Koch is one of the most serious pests in greenhouses and has developed high resistance to many classes of acaricides rapidly. Three T. urticae populations were collected from vegetable greenhouses in Antalya and Muğla, Turkey. These populations showed high resistance levels to abamectin ranging between 223 and 404 fold compared to a susceptible population. The interaction of some synergists (piperonyl butoxide; PBO, diethyl maleate; DEM and S-benzyl O,O-diisopropyl phosphorothioate; IBP) with abamectin was analyzed showing possible implication of esterases in resistances in the three populations studied. The activities of esterase, glutathione S-transferase (GST) and cytochrome P450 (p450) was determined using α-naphthyl acetate, 1-chloro-2,4 dinitrobenzene (CDNB) and 7-ethoxycoumarin (7-EC) as substrates, respectively. In all field populations, esterase, glutathione S-transferase and P450 activities were higher, when compared to the susceptible population (GSS). The presence of known abamectin resistance target site mutations (G314D and G326E) on the glutamate gated chloride channels was also examined. However, no target site–resistance mutation was detected in all three populations. According to our results, detoxification enzymes, but no target site intensivity seem to play role in abamectin resistance in field T. urticae populations from Turkey.
The protease-activated receptor 2, expressed on keratinocytes but not on melanocytes, has been ascribed functional importance in the regulation of pigmentation by phagocytosis of melanosomes. Inhibition of protease-activated receptor 2 activation by synthetic serine protease inhibitors requires keratinocyte-melanocyte contact and results in depigmentation of the dark skinned Yucatan swine, suggesting a new class of depigmenting mechanism and agents. We therefore examined natural agents that could exert their effect via the protease-activated receptor 2 pathway. Here we show that soymilk and the soybean-derived serine protease inhibitors soybean trypsin inhibitor and Bowman-Birk inhibitor inhibit protease-activated receptor 2 cleavage, affect cytoskeletal and cell surface organization, and reduce keratinocyte phagocytosis. The depigmenting activity of these agents and their capability to prevent ultraviolet-induced pigmentation are demonstrated both in vitro and in vivo. These results imply that inhibition of the protease-activated receptor 2 pathway by soymilk may be used as a natural alternative to skin lightening.
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Product |
Annual Subscription License |
Non-Expiring License |
SoftMax Pro Standard Software
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One year subscription to the latest version of SoftMax Pro 7. Includes one activation key, which expires after one year. Annual renewal. Part number:
Special pricing for verified academic institutions. For contact numbers outside the US, please visit our contact page. |
Non-expiring license for the latest version of SoftMax Pro 7. Includes four activation keys.
Special pricing for verified academic institutions. For contact numbers outside the US, please visit our contact page. |
SoftMax Pro Importer XLSRequires SoftMax Pro 6.4.1 or higher |
One year subscription to enable import of data into SoftMax Pro from Excel based template. Includes one activation key, which expires after one year. Annual renewal. Part number: (Click below to purchase/download) |
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SoftMax Pro Importer XLS and XMLRequires SoftMax Pro 6.4.1 or higher |
One year subscription to enable import of data into SoftMax Pro from Excel based template or XML file. Includes one activation key, which expires after one year. Annual renewal. Part number: (Click below to purchase/download) |
Non-expiring license to enable import of data into SoftMax Pro from Excel-based template or XML file. Includes one activation key for one computer. Part number: (Click below to purchase/download) SMP.IMPORT.XLS.AND.XML.NONEXP |
SUCCESS STORY
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