SpectraMax 340PC384 Microplate Reader

Capture 96- and 384-well microplate absorbance data that fuels your imagination


Be inspired by answers to an infinite range of research questions with the SpectraMax® 340PC384 Microplate Reader, our tunable visible absorbance reader for 96- and 384-well microplates. Acquire data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Our SpectraMax® 340PC384 Microplate Reader comes with temperature control up to 45 °C, a robotics-compatible interface, and  SoftMax® Pro Data Analysis Software. PathCheck® Sensor technology automatically measures the liquid depth of each well, and can also be used to calculate concentrations without a standard curve and expand the dynamic range of your readings to 4.0 OD. Our SpectraMax 340PC384 Microplate Reader will run so many of your applications, including ELISA, cytoxicity, ß- galatosidase reporter gene assays, and NAD(P)H-based assays.


  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 340-850 nm in 1 nm increments
  • Easy assay optimization: Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine wavelength peaks
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker


Microplate Reader Validation


The SpectraMax 340PC384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Microbial growth
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays


SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature

Softmax Pro Software Setup

SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions    
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment's needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience      
  • Contemporary user community enabling protocol exchange and expertise
    sharing among our numerous users

Softmax Pro Software Data Analysis




* available in select instrument models

General Specifications

Dimensions (in) 8.6 (h) x 22.8 (w) x 15 (d)
Dimensions (cm)   22 (h) x 58 (w) x 38 (d)
Weight 30 lb (13.6 kg)
Power source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 340 - 850 nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth ≤ 2.0 nm
Wavelength Accuracy ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Stray Light ≤ 0.07% at 340 nm
OD ≥ 3.15
Light Source Xenon flash lamp
Microplate Read Time - Endpoint 96-well: 9 seconds
384-well: 29 seconds
Microplate Read Time - Kinetic 96-well: 9 second interval
384-well: 29 second interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C


SpectraMax® 340PC384 Microplate Reader



Part Number

SpectraMax 340PC384 Microplate Reader 340PC 384
 SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756

Number of Citations*: 9,100

Latest Citations:
For a complete list, please click here.

Prevention of arterial thrombosis by edoxaban, an oral factor Xa inhibitor in rats: monotherapy and in combination with antiplatelet agents

Y Honda, C Kamisato, Y Morishima - European Journal of Pharmacology, 2016 - Elsevier
... The reaction was initiated by adding 40 μl of a chromogenic substrate of FXa, S-2222 (750 μM).
The reaction velocity was measured with a SpectraMax Plus 340 (Molecular Devices Co,
Sunnyvale, CA, USA) at 405 nm from 2 min to 10 min at room temperature. ...

[HTML] Enhancement of the CXCL12/CXCR4 axis due to acquisition of gemcitabine resistance in pancreatic cancer: effect of CXCR4 antagonists

M Morimoto, Y Matsuo, S Koide… - BMC …, 2016 -
... After 72 h of incubation, absorbance was measured at 450 nm in each well using a SpectraMax
340 spectrophotometer (Molecular Devices, CA, USA). ... After 72 h of incubation, absorbance was
measured at 450 nm in each well using a SpectraMax 340 spectrophotometer. ...

A new glycotoxins inhibitor attenuates insulin resistance in liver and fat cells

SK Afridi, MF Aftab, M Murtaza, S Ghaffar… - Biochemical and …, 2016 - Elsevier
... of URM-II-81 for 24 h at 37 °C with 5% CO 2 . MTT solution was added and cells were incubated
for 4 h. Formazan formed during incubation was solubilized by the addition of dimethyl sulfoxide
and quantified using ELISA reader (Molecular devices SpectraMax 340, USA). 2.5. ...

[HTML] Developing a Gene Biomarker at the Tipping Point of Adaptive and Adverse Responses in Human Bronchial Epithelial Cells

JM CurrierWY Cheng, D Menendez, R Conolly… - PloS one, 2016 -
... Gaithersburg, MD). Absorbance of solubilized formazan product was quantified with
SpectraMax 340 microplate reader at 570 nm using a reference wavelength of 630
nm (Molecular Devices, Sunnyvale, CA). NRF2 activation assays. ...

Miltefosine inhibits Candida albicans and non-albicans Candida spp. biofilms and impairs the dispersion of infectious cells

T Vila, K Ishida, SH Seabra, S Rozental - International Journal of …, 2016 - Elsevier
... The minimum concentrations that inhibited 50% and 90% of fungal growth (IC 50 and IC 90
values, respectively) were determined by visual analysis and by spectrophotometric readings
at 492 nm (SpectraMAX ® 340; Molecular Devices, Sunnyvale, CA). ...

[HTML] Porcine placenta hydrolysates regulate calcium disturbance in MC3T3-E1 osteoblastic cells

HY Lee, HR Kim, SY Park… - BMC …, 2016 - bmccomplementalternmed. …
... AFC, Sigma-Aldrich) in a final volume of 100 μL and incubated at 37 °C. Caspase-3 activity was
analyzed continuously by monitoring fluorogenic AFC release at 37 °C. Subsequently, substrate
cleavage was monitored at 405 nm using a SPECTRAmax 340 microplate reader. ...

[HTML] Maternal High Fat Diet Alters Skeletal Muscle Mitochondrial Catalytic Activity in Adult Male Rat Offspring

CA Pileggi, CP Hedges, SA Segovia… - Frontiers in …, 2016 -
... All assays were performed using the Molecular Devices Spectramax-340 96-well microplate
reading spectrophotometer at 25°C. CS and CPT1 activity were determined by measuring
absorbance at 412 nm in 50 mM Tris-HCl (pH 8.0) with 0.2 mM DTNB. ...

Toxicological Effects of Differently Polluted Dam Waters Spiked with Pesticides on Freshwater Snails Lymnaea Natalensis

MR Delfina, D Tapfuma, S Mnkandla… - International Journal of …, 2016 -
... The rate of production of a complex between thiocholine and DTNB was followed at 25ºC for
5 minutes at 412 nm using the SpectraMax 340 pc plate reader. ... The absorbance was measured
at 405 nm for 5 minutes at 25 °C using the SpectraMax 340 pc plate reader. ...

Activation of exogenous fatty acids to acyl-acyl carrier protein cannot bypass FabI inhibition in Neisseria

J Yao, DF Bruhn, MW Frank, RE Lee… - Journal of Biological …, 2016 - ASBMB
... The enzyme reactions were 100 μl in volume and performed and monitored in Costar UV
half-area 96-well plates with a SpectraMax 340 instrument taking 340-nm readings at 10-s
intervals at 37 °C. The substrate crotonyl-ACP was synthesized as described previously using ...

[HTML] Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

C Cortesia, T Bello, G Lopez, S Franzblau… - Brazilian Journal of …, 2016 - Elsevier
... Optical density at 600 nm (OD 600 ) was measured in a 96 well spectrophotometer (Molecular
Devices Spectramax 340), and fluorescence (485 nm excitation and 535 nm emission) was
measured in a 96 well plate fluorimeter (Molecular Devices SpectraMax, GeminisXS). ...



* as of December 6, 2016. Source: Google Scholar.