Zika Virus Activates De Novo and Cross-Reactive Memory B Cell Responses in Dengue-Experienced Donors

Rogers TF, et al., 2(14). pii: eaan6809, Sci Immunol., 2017

Zika virus (ZIKV) is closely related to dengue virus (DENV), suggesting an understanding of how previous DENV exposure affects the B cell response to ZIKV will be important for the development of therapeutic interventions for DENV-immune populations. Described herein is a longitudinal study of the ZIKV-induced B cell responses in three DENV-experienced donors using single B cell cloning and large-scale antibody isolation. Bio-Layer Interferometry (BLI) was used to study the binding affinities of IgGs. A Pall ForteBio Octet HTX instrument equipped with Anti-Human IgG Fc (AHQ) Biosensor probes was used to perform all BLI experiments. High-throughput binding affinity screening was performed by immobilizing IgGs on AHQ sensor tips and dipping them in ZIKV E, DENV1-4 E, or NS1 protein samples for an association step, followed by a dissociation step in PBSF buffer. BLI data obtained were fitted to a 1:1 binding model and the kinetic rate constants (association and dissociation rate constants) and the equilibrium dissociation constants (KD values) were determined. Collectively,the results of this investigation have significant implications for the development of ZIKV vaccines for DENV-immune individuals.

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