XPB Helicase Regulates DNA Incision by the Thermoplasma acidophilum Endonuclease Bax1

HM Roth, et al., 11(3), 286-293, DNA Repair, 2012

In this work, the endonuclease activity of Bax1 is characterized, alone and in combination with XPB helicase. Binding of Bax1 and of the XPB/Bax1 complex to different DNA structures was studied using the Octet RED platform. Biotinylated dsDNA strands with blunt ends or overhangs were immobilized on Streptavidin biosensors and kinetic rate constants for Bax1, XPB, and the XPB/Bax1 complex were determined. The Octet data demonstrated that XPB facilitates binding of Bax1 to DNA.

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