Two ScFv Antibody Libraries Derived from Identical VL-VH Framework with Different Binding Site Designs Display Distinct Binding Profiles

Huovinen T, et al., 20(10), 683-693, Protein Eng Des Sel, 2013

In this study of diverse antibody libraries, the authors demonstrate that by targeting different residues of the same antibody gene, it is possible to create sublibraries with distinct profiles in terms of the number and affinities of the library clones against different-sized antigens. Apparent affinities for anti-streptavidin clones were measured on the BLItz system using Streptavidin biosensors.

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