The Regulation of Apoptosis by the Downstream Regulatory Element Antagonist Modulator/Potassium Channel Interacting Protein 3 (DREAM/KChIP3) Through Interactions with Hexokinase I

Craig, T A, et al., 433(4), 508-12, Biochem Biophys Res Commun, 2013

This study explores the mechanism by which the DREAM protein alters apoptosis in neuronal cells. DREAM was found to interact with brain hexokinase I in a calcium-dependent manner. The BLItz system was used to characterize binding of DREAM to hexokinase I. Biotinylated DREAM was loaded onto Streptavidin biosensors and incubated with hexokinase I in the presence of either calcium chloride or EGTA. Association and dissociation constants were determined.

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