The N-terminus of the Human RecQL4 Helicase is a Homeodomain-like DNA Interaction Motif

Ohlenschlager, O., et al., 40(17), 8309-24, Nucleic Acids Research, 2012

Mutations in RecQL4 helicase are associated with Rothmund-Thomson and other syndromes. The authors use the Octet RED96 system to study DNA binding characteristics of the N-terminus of RecQL4 (RecQL4_N54). Three forms of biotinylated DNA (Y-shaped, dsDNA and ssDNA) are immobilized on Streptavidin biosensors and then exposed to RecQL4_N54 to determine dissociation constants. The authors note that these experiments could not be performed on their Biocore 2000 system due to nonspecific binding problems.

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