The Molecular Basis for Mucosal-associated Invariant T cell Recognition of MR1 Proteins

Lopez-Sagaseta, J, et al., 110(19), E1771-8, Proc Natl Acad Sci U S A, 2013

This study elucidates the structure of MAIT TRC (mucosal-associated invariant T-cell receptor) bound to bovine MR1 (MHC related-1 protein). The affinity of the F7 MAIT TCR for single bovine MR1 mutants expressing human residues was determined using the BLItz system and compared to the wild-type affinity. Recombinant 12xHIS tagged MR1 proteins were loaded onto Ni-NTA biosensors for these experiments.

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