The Crystal Structure of S. cerevisiae Sad1, a Catalytically Inactive Deubiquitinase that is Broadly Required for pre-mRNA Splicing

Hadjivassiliou H, Rosenberg OS, and Guthrie C, 20(5):656-69, RNA, 2014

The authors performed structural and functional analysis on the zinc finger ubiquitin binding domain (Znf-UBP) of Sad1, an essential splicing factor from Saccrharomyces cerevisiae to better understand the mechanisms of spliceosome assembly and the regulation of pre-mRNA splicing. Binding studies were done on the Octet384 system using Streptavidin biosensors.

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