Structure and Receptor Binding Preferences of Recombinant Human A (H3N2) Virus Hemagglutinins

Yang H, et al., 477:18-31, Virology, 2015

The article describes glycan microarray analysis of recombinant A(H3N2) hemagglutinins (HAs) for their structural differences and changes in receptor binding. The authors cloned, expressed, and analyzed the glycan preference of 19 HAs chosen from WHO vaccine library that were observed from 1968 to 2012. An Octet RED instrument equipped with Streptavidin biosensors was used to perform glycan binding analyses. Biotinylated glycans captured on to Streptavidin biosensors were screened against recombinant HAs.

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