Structure and Protein-protein Interactions of Methanol Dehydrogenase from Methylococcus Capsulatus (Bath)

Culpepper M, Rosenzweig A, 53(39):6211-9, Biochemistry, 2014

Based on several biochemical and X-ray crystallography studies on two major protein families involved in the first step of the metabolic pathway in methanotrophs (gram-negative bacteria), the authors are trying evaluate the possible existence of a metabolic super-complex between methane monooxygenases (MMOs) and methanol dehydrogenases (MDHs). BLI experiments carried out using a BLItz system were able to estimate the protein-protein interactions between MDH and pMMO as well as between MDH and spmoB (truncated recombinant periplasmic domains of pMMO). Each BLI assay involves immobilization of recombinant MDH onto amine reactive second-generation (AR2G) biosensors followed by the association with a particular concentration of pMMO.

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