Structural Optimization and De Novo Design of Dengue Virus Entry Inhibitory Peptides

Costin, J.M., et al., 4(6), e721, PLoS Neglected Tropical Diseases, 2010

Predictive strategies together with computational optimization of binding "pseudoenergies" were utilized to design two antiviral peptide sequences against the Dengue-2 virus envelope protein. Kinetic binding analysis was performed on the Octet QK system by immobilizing biotinylated DENV-2 S1 E protein onto Streptavidin biosensors, and incubating with the inhibitory peptides. The Octet platform data, along with supporting data, confirmed that these two peptides interact directly with DENV-2 E proteins and are entry inhibitors.

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