Structural and Biophysical Analysis of Bst-2/Tetherin Ectodomains Reveals an Evolutionary Conserved Design to Inhibit Virus Release

Swiecki, M., et al., 286(4), 2987-2997, J Biological Chemistry, 2011

BST-2/tetherin is a host antiviral molecule that inhibits the release of enveloped viruses from infected cells. This paper presents detailed sturctural and biophysical analysis of this protein. In one series of experiments, refolding mouse BST-2 ectodomain was assessed for proper folding using anti-mBST-2 mAb 927, a mAb raised against cell surface-expressed mouse BST-2. Biotinylated mAb 927 was immobilized on Streptavidin biosensors for these studies. Binding of various concentrations of mouse BST-2 ectodomain were monitored using the Octet system.

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