Small-molecule Inhibition of Human Immunodeficiency Virus Type 1 Replication by Targeting of the Interaction between Vif and ElonginC

Zuo, T., et al., 86(10), 5497-507, Journal of Virology, 2012

The objective of this study was to design a small molecule that disrupts binding of HIV viral infectivity factor Vif to the cellular host protein ElonginB/C, and thus inhibit HIV replication. A potent small molecule inhibitor was identified, and its effects on Vif-ElonginB/C binding were characterized using the Octet RED96 system. Inhibition was studied using recombinant ElonginB/C immobilized on Streptavidin biosensors and incubated with Vif protein in the presence or absence of the small molecule inhibitor.

Read More