Shark Attack: High affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation

Zielonka S, et al., pii: S0168-1656(14)00204-1, J Biotechnol, 2014

The authors described the use of yeast surface display library screening to generate and identify high-affinity binders to antigen-specific shark vNAR (new antigen receptor) domains in vitro. They demonstrated specific binding of the antibodies without compromising the desirable high thermostability of the vNAR scaffold. Binding kinetics were determined on the Octet RED96 system using Streptavidin biosensors.

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