Semi-continuous, Label-free Immunosensing Approach for Ca2+-based Conformation Change of a Calcium Binding Protein

Paek SH, et al., 139(15):3781-9, Analyst, 2014

The study detailed the use of BLI to directly measure the interaction of small molecules and calcium binding to a calcium-binding protein (CBP) designated GGBP for analysis of food samples. This novel and reproducible approach of label-free sensing of calcium levels specifically in milk samples suggests that food components can be analyzed with minimal handling and could possibly lead to further advances in automated analysis. Detection of calcium levels was performed on the Octet RED system using Anti-mouse Fc capture (AMC) biosensors.

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