Selection of a High-affinity WW Domain Against the Extracellular Region of VEGF Receptor Isoform-2 from a Combinatorial Library using CIS Display

Patel, S, et al., 26(4), 307-15 , Protein Eng Des Sel, 2013

The authors demonstrate that the Pin1 WW domain motif can be used as a framework for engineering and selection of high-affinity binders. The Octet RED system was used for real-time binding assays between chemically synthesized peptides and human VEGFR-2-Fc fusion, VEGFR-1-Fc fusion or VEGFR-3-Fc fusion. The biotinylated peptides were loaded onto Streptavidin biosensors for these studies.

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