Robo4 Maintains Vessel Integrity And Inhibits Angiogenesis By Interacting With UNC5B

Koch, A.W., et al., 20(1), 33-46, Developmental Cell, 2011

In this publication, a library of over 1900 purified protein constructs containing Fc or histidine tags was screened for binding to Robo4-Fc with the Octet RED platform - a novel example of a protein-protein interaction screen. Robo4-Fc was loaded to saturation onto anti-human Fc biosensors, then washed in HBS-P buffer for 30 s and placed for 3 min in wells containing library proteins (5 mg/mL).

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