Reconstitution of a Frizzled8-Wnt3a-LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6

Bourhis, E., et al., 285 (12), 9172-9179, Journal of Biological Chemistry, 2010

To dissect Wnt signaling at the cell surface, the authors characterized interactions of the Wnt signaling proteins LRP6, Wnt3a, Wnt9b, Fz8, and DKK1 using the Octet RED platform with Streptavidin and Anti-Human Fc biosensors. Binding constants for several binary complexes were reported. Notably, the authors were able to reconstitue a Fz8-Wnt3a-LRP6 ternary complex on the surface of the Anti-Human FC biosensor and report new detailes about its molecular arrangement and regulation by DKK1.

Read More