Recombinant Fab Expression and Secretion in Escherichia Coli Continuous Culture at Medium Cell Densities: Influence of Temperature

Carmona, E. et al., Process Biochemistry, 2012, 47 (3), 446-452, 2012

The aim of this work was to study the production of a model Fab in an E. coli continuous culture at medium cell densities. Temperature was chosen as the working parameter to perform a systematic analysis of the effect of operational factors on the process behavior at cell densities closer to those typically used under bioprocess conditions. An Octet QK system with Streptavidin biosensors was used to characterize Fab fragments produced at different temperatures for functional binding activity.

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