Rapid Homogenous Time-Resolved Fluorescence (HTRF) Immunoassay for Anthrax Detection

Cohen N, et al., doi: 10.1007/s10895-014-1354-7, J Fluoresc, 2014

This report described the development and testing of a new rapid and sensitive homogenous time-resolved fluorescence (HTRF) assay for the detection of bacterial toxin-PA and bacterial spores from Bacillus anthracsis, which can induce acute anthrax disease. Rapid, accurate and sensitive diagnosis of anthrax at early stages of the disease is critical to allow for an effective treatment. Binding characterization of the antibodies used in the assay development was performed on the Octet RED system and Streptavidin biosensors were used.

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