Rapid Detection of [Beta]-lactam Antibiotic Residue in Milk Using Biolayer Interferometry

Liu, X, et al., 3(1):9-11,15, Agricultural Biotechnology, 2014

The article describes a complementary method that could be used for qualitative detection of β-lactam antibiotics in cow milk. The β-lactam antibiotics such as penicillins, cephalosporins, cephamycins, and carbapenems are widely used to treat bovine mastitis. Traces of these antibiotics could remain in milk if the antibiotics are administered improperly or without adhering to proper guidelines. Therefore techniques that have higher sensitivity, higher specificity, and a high-throughput are required to screen milk samples on a regular basis. The BLI platform used in this method development has an ampicillin-BSA conjugate tethered via hydrophobic interactions on to aminopropylsilane (APS) biosensors. The conjugate was then bound to colloidal gold labeled β-lactam antibiotic receptor. The receptor bound biosensors were subsequently used to qualitatively detect β-lactam antibiotic residues in milk. The method, although a qualitative approach, has a two-fold higher sensitivity and better throughput compared to existing immuno-chromatographic approaches. An Octet RED system was used in the assay development.

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